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Biomedical subjects

W LATHEM

Publications and source records attributed to W LATHEM.

18 recordsLinked to original sources
PubMed1960

The binding of myoglobin by plasma protein.

When added to dog plasma in vitro and in vivo, myoglobin was bound to plasma protein in a concentration which, maximally, averaged 21 +/- 6 mg. per cent. Electrophoretically, bound myoglobin was separated from free myoglobin and migrated between alpha-2 and beta globulin. The electrophoretic characteristics of protein-bound myoglobin were similar to, although not identical with, those of protein-bound hemoglobin. The maximal binding capacity of plasma for myoglobin was less than for hemoglobin, which averaged 123 mg. per cent. At concentrations below the maximal binding capacity, from 15 to 50 per cent of the myoglobin was in the free, unbound state, differing from hemoglobin which was completely bound at all concentrations below the binding capacity. When myoglobin and hemoglobin were added together to plasma, hemoglobin appeared to interfere with the binding of myoglobin or to replace it at the binding sites. Myoglobin, however, did not appear to interfere with the binding of hemoglobin. These observations suggested that myoglobin and hemoglobin were bound at least in part by the same protein. When myoglobin was given intravenously, free myoglobin was excreted in the urine, whereas protein-bound myoglobin was not excreted. This suggests that protein-binding contributes to or determines the apparent renal threshold to myoglobin.

Animals↗
PubMed1956

Observations on renal protein metabolism; renal venous amino nitrogen content before and following hemoglobin infusions in dogs.

No significant difference was demonstrated in the free and total alpha amino nitrogen content of arterial, renal venous, and inferior vena caval blood of normal anesthetized dogs during fasting and following the administration of 3 to 6 gm. of hemoglobin. These results do not provide evidence of renal protein reabsorption and metabolism in the dog under the conditions employed in this study.

Animals↗