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Biomedical subjects

W Leyko

Publications and source records attributed to W Leyko.

At least 73 records · Page 4Linked to original sources
PubMed1977

E.s.r. radiation studies of erythrocyte membrane haemoglobin interaction.

The dependence of the yield of free radicals in gamma-irradiated, freeze-dried erythrocyte membranes on their haemoglobin content was studied. A non-monotonous relationship was found--different from that observed in mixtures of freeze-dried membranes and haemoglobin, which suggests the existence of radiation-energy transfer between the membranes and bound haemoglobin.

Electron Spin Resonance Spectroscopy↗
PubMed1976

Effect of UV-irradiation of shifts of energy-rich phosphate compounds: ADP, ATP and AXP in human red blood cells represented by a trigonometrical polynomial.

This paper deals with the effect of UV-irradiation on metabolism of energy rich adenine, esters: ADP, ATP and AXP in human red blood cells in vitro. Preparation, separation and quantitative determination of these compounds were performed by the method of BARTLETT, using a step concentration gradient with an HC1-NH4C1 chloride system. Blood was irradiated in vitro for 30, 60, 90, 120, 150, 180, 210, 240, 270 and 300 min. Mathematical methods based on KOLMAGOTOV's theorem were applied for continuous, not stepwise description of dynamics of changes of ADP, ATP and AXP in red blood cells following UV-irradiation. The obtained curves illustrate dynamic, time-dependent changes of levels of the investigated compounds and point out that the method of BARTLETT used for determination of concentrations of ATP and AXP need further refinements, especially in applications to such biological systems and functional states in which slight shifts of these compounds occur.

Adenine Nucleotides↗
PubMed1975

Effect of UV-irradiation in vitro on adenin nucleotides metabolism, Na+ and K+ concentration, osmotic properties and submicroscopic structure of pigeon red blood cells.

Effect of UV-irradiation in vitro on metabolism of adenine nucleotides: ADP, ATP and AXP, osmotic properties and submicroscopic structure of nucleated pigeon red blood cell was investigated. Irradiation carried out for 60, 120, 180, 240 and 300 minutes. Hanau S-500 lamp with efficiency 4.34-10(8) erh/sec was used. A decrease of ATP content with a stimultaneous increase of ADP and AXP contents and a rather constant level of the sum of adenine compounds was observed. UV-irradiation caused a decrease of reversal of hemolysis and osmotic resistance to hypotonic NaCl solutions. An equivalent exchange of Na+ and K+ ion and an increase of hematocrit value, following UV-irradiation was observed. Electron microscope studies demonstrated changes of ultrastructure concerning both cell nucleus and thickness and granulation of cell membrane.

Adenine Nucleotides↗