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W Liu

Publications and source records attributed to W Liu.

At least 793 records · Page 44Linked to original sources
PubMed1996

[Applications of the island trapezius myocutaneous flap in oral maxillofacial surgery].

The experience in immediate reconstruction of oral maxillofacial defects of 23 cases by island trapezius myocutaneous flaps from April 1985 to July 1993 is presented. The smallest flap was 6 x 4 cm and the largest 12 x 8 cm, with all the cases successful. The selection of the vascular pedicle of the myocutaneous flaps, the technique of the operation, the advantages and the disadvantages of the method are discussed.

Adult↗
PubMed1996

[The effect of gamma-interferon on hepatic fibrosis in schistosomiasis japonica].

OBJECTIVE: To study the effect of gamma-IFN on hepatic fibrosis in schistosomiasis japonica. METHODS: The amount and distribution of gamma-IFN and extracellular matrix, including fibronectin (FN), laminin (LN), I and II collagen which were used to judge the suppressing effect of gamma-IFN on hepatic fibrosis as markers of dynamics of extracellular matrix after administration of recombinant gamma-IFN, in liver obtained at different stages from S. japonicum-infected mice were determined by immunohistochemical streptavidin biotin-peroxidase complex method. RESULTS: The amount of gamma-IFN in liver reached a peak (3 mice respectively reached 2, 3 and 4 grade) at the 16th week after infection, which was higher than the levels of control groups and infected-mice at the 8-12th week (chi 2 = 15.39, 7.50, P < 0.01), and the majority of gamma-IFN was distributed round egg granuloma. FN and LN, and I and II collagen increased to 1 grade, individual 2 grade, at the 8th week after infection, which were higher than control (chi 2 = 12.44-15.39, P < 0.01), and were lineally distributed round egg granuloma. With chronicity and decrease of gamma-IFN, however, FN, LN and I, II collagen gradually increased, and reached a peak (over 70% infected mice reached 3-4 grade) respectively at the 20th week and 24th week, became wide, thick and retiform and deposited round and in egg granuloma. After administration of gamma-IFN, only 3 infected mice had 2 grade of FN at the 20th week, two mice had respectively 3 grade of type I and II collagen at the 24th week, and none of them reached 4 grade, which were significantly less than those in the untreated group at the same stage (chi 2 = 10.77, 5.05, 7.20, P < 0.01-0.05). CONCLUSION: The results indicate that gamma-IFN may play an important role in opposing the inflammatory response of egg granuloma, decreasing secretion and deposition of extracellular matrix in liver and suppressing hepatic fibrosis.

Animals↗
PubMed1996

Analysis of thymoma excision in patients with myasthenia gravis.

OBJECTIVE: To study the clinical characteristics of myasthenia gravis (MG) with thymoma and the effect of tracheostomy at the early stage after operation. METHODS: Thymoma excision and selective tracheostomy were performed on 46 patients with MG from March 1983 to December 1994. RESULTS: There were 15 patients with benign thymomas and 31 patients with malignant thymomas. MG crises were seen in 52.2% of patients postoperatively, most in type IIb and III. The mortality of in-patients after operation was 10.9%. Thirty-nine patients with thymoma were followed up for 6 months to 12 years after being discharged from hospital. The MG symptoms were relieved in 33.3%, improved in 20.5% and unchanged in 2.6% of patients. 38.5% of patients died of the disease. CONCLUSION: The characteristics of MG with thymoma are, more severe condition of the patient, rapid advance, and more MG crises before and after operation. The perioperative mortality from MG with thymoma is higher and its prognosis is unfavourable. It is very important to treat severe cases of MG with thymoma by tracheostomy with thymoma excision at the early stage after the operation. This is an effective method to prevent and treat MG crises as well as to lower the perioperative mortality.

Adult↗
PubMed1996

[Study of immunoregulation by interleukin-1 receptor antagonist in NZB/W F1 mice].

OBJECTIVE: It is known that the development of systemic lupus erythematosus (SLE) is associated with the highly activated B cells. Interleukin-1 (IL-1) can obviously promote the proliferation and secretive activity of B cells. Moreover, interleukin-1 receptor antagonist (IL-1ra) can block the effect of IL-1 by specifically combining with IL-1 receptor, we, therefore studied the immunoregulative action of IL-1ra in lupus-like NzB/w F1 mice to detect any possible way to prevent lupus nephritis. METHODS: 12 femal NZB/W F1 mice of 13 weeks (Weight: 30-35 g) were divided randomly into 2 groups. Each mouse in the treated group was intraperitoneally injected with IL-1ra once per 2 weeks for 3 times by the dosage of 100 micrograms (0.1 ml) per time, while in the control group injected with 0.1 ml normal saline. All the mice were killed at the age of 9 months and the immunologic function was detected. RESULTS: This dosage could not prevent absolutely the development of lupus nephritis, but the renal damage was alleviated and the urine protein decreased. Moreover, it could improve the immunofunction by significantly reducing the levels of serum IL-1 alpha and obviously increasing the activities of NK cells and IL-2 induced by ConA in mononuclear cells of spleen. There was no significant difference on the levels of serum IL-6 and TNF-alpha between the treated group and control group. CONCLUSION: IL-1ra has certain regulatory effect on the immunologic function of lupus-like NZB/W F1 mice.

Animals↗
PubMed1996

[Analysis of DNA methylation pattern of c-myc oncogene in hepatocellular carcinoma].

OBJECTIVES: To assess whether hypomethylation of c-myc gene occours in HCC and to determine its clinical significance. METHODS: The methylation pattern at 3' end of c-myc gene was analyzed by Southern blot using methylation sensitive restrcition endonuclease enzymes Hpa I and Msp I in six normal liver tissues and 18 HCCs. RESULTS: At least 5 CCGG sites were methylated at 3' end of c-myc gene in 6 normal liver tissues. However, the normal hypermethylation pattern was absent in 11 hepatocellular carcinoms (HCC) which showed different degree of hypomethylation even if among subclonal cells of same tumour tissues. The hypomethylation was observed more often in markedly advanced HCC with metastasis and poorly differentiated histological grade. CONCLUSION: Scattered CpG at 3' end of c-myc gene was methylated in normal liver tissues. Methyl groups were deleted in different extent in HCC, which can lead to abnormal expression of c-myc gene. The hypomethylation of c-myc can reflect the malignlant extent of HCC and may serve as a useful prognostic indicator in clinical practice.

Carcinoma, Hepatocellular↗
PubMed1996

[The effect of interferon in combination with ribavirin on the plus and minus strands of hepatitis C virus RNA in patients with hepatitis].

The effect of interferon in combination with ribavirin on the plus and minus strands of hepatitis C virus (HCV) RNA in patients with chronic hepatitis C (CHC) was studied by means of nested RT-PCR. The results showed that in those who respond to the combination of antiviral therapy, their increased serum ALT levels decreased to normal range, but more than half (55.56%) of these patients relapsed 24 weeks after cessation of the antiviral therapy. The positive rate of the plus strand HCV RNA in serum (92.31%) decreased significantly to 38.46% (P < 0.005) and that of the minus strands HCV RNA in peripheral blood mononuclear cells (PBMC, 76.92%) to 38.46% (P < 0.05) at the end of the treatment, but little effect on the plus strand HCV RNA in PBMC in these patients was found. Relapse occurred in patients whose plus and minus strands of HCV RNA in PBMC remained positive during treatment. These data indicated that absence of HCV RNA in serum do not mean complete clearance of HCV and can not predict a sustained therapeutic response. For evaluating the antiviral effect and prognosis it is essential to measure the plus and minus strands of HCV RNA in serum and PBMC simultaneously. The results of the combined antiviral therapy were similar to that of single interferon treatment; it seems that rib-a-virin do not enhance the antiviral effect of interferon.

Adult↗
PubMed1996

[Study of the amplification and expression of c-erbB2 oncogene in epithelial ovarian tumors].

Protooncogene c-erbB2 amplification and expression were studied in 62 cases of epithelial ovarian tumor by using immunohistochemical method and in situ hybridization. c-erbB2 gene amplification was observed in 30 of 47 ovarian cancers (63.8%) and in none of 15 benign tumors. The gene expression was found in 38 of the former and in 9 of the latter tumors. The gene amplification showed significant difference between malignant and benign tumors (P < 0.01). But for gene expression, only the strongly positive staining demonstrated significant difference (P < 0.01). There was correlation between c-erbB2 gene amplification and expression in epithelial ovarian carcionoma. The status of c-erbB2 gene amplification and expression had no relationship with histopathological types of ovarian carcinoma. The results indicate that there is extensive expression of c-erbB2 in epithelial ovarian tumors and it is over-expressed in ovarian carcinoma. Gene amplification is perhaps only one way by which c-erb2 gene is activated, and its overexpression might be one of the factors that promotes epithelial ovarian cancer development.

Carcinoma, Endometrioid↗
PubMed1996

[The effects of pituitory-adrenal system in the EA-modulating immune responses].

The aim of this work was to investigate the role of pituitory-adrenal system in the electroacupuncture modulating immune responses. We found that the multiple immune responses were declined in mice receiving cortisol ip. treatment, whereas it could be increased in mice of adrenalectomy. The IL-2, LTT contents declined by cortisol could be restored to normal levels by EA, however, the hemolytic antibody and hemoagglutinating antibody titer declined by cortisol were not affected by EA. In the adrenalectomized mice, except spleen LZM was declined, all the other immune parameters such as spleen PFC, IL-2, LTT, serum IgG and thymus LZM contents were increased. The EA could restore the declined spleen LZM in adrenalectomized mice to normal, but the other elevated immune parameters by adrenalectomy were not affected by EA, the immune levels were almost the same as that in the single adrenalectomy group. The above results indicate that pituitory-adrenal system may principally play an inhibitory effect in hte EA-modulating immune responses.

Animals↗
PubMed1996

[A comparative study of apoptosis and proliferation of nasal T cell lymphoma].

A quantitative evaluation and statistical analysis of apoptosis and proliferation in 30 cases of MMR was carried out by means of in situ end-labelling (ISEL) of fragmented DNA technic and expression of Ki-67 antigen in routine paraffin sections with comparison of clinicopathology and prognosis. 28 patients had been followed for 6 to 29 months (mean 12.4 months) and 8 (28.5%) died of relapse and idsease progression. The results showed that (1) the number of apoptotic cells was found to correlate with the ratio of Ki-67 positive cells (P < 0.0063). No significant association of mitotic figures, Ki-67 labelled cells and the expression of bel-2 oncoprotein was observed. The results also did not suggest a correlation of proliferation or apoptosis with the stage of the disease at presentation. (2) statistical analysis revealed a significant impact of Ki-67 positive cells (P < 0.0160) on mean survival time and no significant association among apoptosis, morphologic changes and the mean survival time was found in this group of cases. The relation between apoptosis, proliferation, expression of bcl-2 oncoprotein and EBV infection in related lymphomas were discussed.

Adult↗
PubMed1996

[Experimental studies on damage to acoustic organ in guinea pigs caused by imitated coal mining noise].

To study the damage to the hearing of miners under the shaft caused by noise and its recovery, an experiment with imitated noise was carried out in guinea pigs. The animals were divided into five groups with exposure to imitated underground noise of 117 dB (A) for one hour for one, two, four, eight and 16 days, respectively, and one control. Thresholds of cortex reaction and evoked electric reaction of brain stem were detected before and after exposure. And, ultrastructural changes in cochlear hair cells were observed by scanning and transmission electron microscopy. Results showed that exposed noise level and length since removing from exposure had significant effects on changes in hearing threshold. Damage to hearing was aggravated with prolonged exposure, to various extent, from changes in temporary threshold shift and permanent threshold shift to recovery from it after removing from exposure. Intracellular structural changes in internal and external hair cells and their injury deteriorated gradually with increase of exposure levels, at worst, to changes in epithelial plate, collapse of Corti's organ and vacuolation, edema and degeneration of cells. It suggests it is important to protect workers form exposure to noise.

Animals↗
PubMed1996

[The relationship of non-Hodgkin's lymphoma with P16 protein expression].

The expression of P16 protein was examined in 45 cases of non-Hodgkin's lymphoma (NHL), 4 cases of reactive hyperplasia of lymph node (RHLN) and 6 cases of normal spleen with immunohistochemical staining. Positive reaction of P16 protein was observed in some lymphocytes of RHLN and splenic nodule and around splenic sinus. The positive rate of P16 protein expression in NHL was 37.8% (17/45). The positive rates of P16 protein expression for the low intermadiate- and high-grade malignancy groups were 66.7% (10/15), 37.5% (6/16) and 7.1% (1/14) respectively. There was a significant difference between the low malignancy NHL and the high malignancy NHL (P < 0.005) in expression rate of P16 protein. It suggests that P16 protein expression may be one of the important parameters in estimating the prognosis of patients with NHL.

Adolescent↗
PubMed1996

[T-cell receptor gene rearrangement analysis of midline malignant reticulosis: a PCR study of 14 cases].

Using PCR method, we analyzed the gene rearrangement of T-cell receptor chain (TCR-beta) in 14 cases of midline malignant reticulosis (MMR). Our results showed that there were non-germline, monoclonal rearrangements of TCR-beta in 12 of 14 cases of MMR (85.7%). In contrast, the same rearrangement pattern was found only in 6 of 10 cases of lymph node based T-cell lymphoma. The cases of reactive lymphoid hyperplasia were of a polyclonal rearrangement pattern. This study suggests that MMR is a monoclonal T-cell neoplasm and PCR method is a rapid, facilitated and useful method for the detection of clonalities of T-cell proliferations.

Gene Rearrangement, beta-Chain T-Cell Antigen Rece↗
PubMed1996

[Calcification mechanism and anticaicification on cardial bioprostheses].

The high incidence of calcification in cardiac bioprostheses greatly shortens their service life. In this study, we found that the calcification of cardiac bioprostheses was related the content of free carboxyl groups in collagen molecule of cardiac bioprostheses. The method of chemical modification of glutaraldehyde (GA) treated cardiac bioprostheses with epoxy chloropropane (EC) was selected. The content of free carboryl groups in collagen molecule was reduced greatly in EC-modified valve tissues, and the calcified content of EC-modified porcine aortic valve, bovine pericardial and porcine pericardial valves was reduced greatly at 2, 4, 6 weeks after subcutaneous implantation in young New Zealand rabbits. There were good physical stability, mechanical tensile strength and no immunologic reactions against the valve in EC modified valves. These findings showed that the anti-calcified valve can be used in clinic.

Animals↗
PubMed1996

[Clarithromycin combined short-term triple therapies for eradication of Helicobacter pylori infection].

UNLABELLED: In order to explore the optimal regimens for the eradication of Helicobacter pylori (Hp), eighty-eight patients with duodenal ulcer (DU) or non-ulcer dyspepsia and Hp infection, defined by culture and histology, received the one-week triple therapy with clarithromycin (Cla), furazolidone (Fur) plus lansoprazole (Lan) or colloidal bismuth subcitrate (CBS). Twenty-eight patients entered the phase one preliminary study, randomized to receive the treatment of Cla 500 mg b.i.d and Fur 200 mg b.i.d plus either CBS 240 mg b.i.d (group I A) or Lan 30 mg b.i.d (group I B). Sixty patients entered the phase two main study, receiving the treatment of Cla 250 mg b.i.d and Fur 100 mg b.i.d plus either CBS 240 mg b.i.d (group II A) or Lan 30 mg q.d (group II B). Five patients (2 in group I A, 2 in group I B, 1 in group II A) were withdrawn because of the side effects of the treatment, and two (in group II A) dropped out. The eradication rates of Hp in group I A, I B, II A, II B were 100.0% (12/12), 91.7% (11/12), 92.6% (25/27) and 90.0% (27/30) respectively. There was no significant difference in the eradication rates of Hp between the treatment groups (P > 0.05). The primary resistant rate of Hp isolates to Cla was 7.5% (4/53) and no patient infected with the strain resistant to Cla succeeded in the eradication of Hp. The healing rates of DU were 94.4% (17/18) in group II A and 100.0% (18/18) in group II B (P > 0.05). The main side effects were nausea, vomiting and taste disturbance. Eighteen of the 28 (64.2%) patients in the phase one study had side effects with a rate significantly higher than that of 5.1% (3/58) in the phase two study (P < 0.001). CONCLUSIONS: (1) Both of the regimens used in the phase two study with low-dose, one week triple therapy are highly effective in the eradication of Hp infection and healing of DU, and are well tolerated. (2) The primary resistance of Hp strains to Cla is the principal factor of treatment failure in these two regimens.

2-Pyridinylmethylsulfinylbenzimidazoles↗
PubMed1996

[Unroofed coronary sinus syndrome].

Unroofed coronary sinus syndrome or coronary sinus septal defect is a rare congenital cardiac anomaly. We performed corrective operations in 9 patients with such condition. Of them, seven patients had the completely unroofed coronary sinus and persistent left superior vena cava (PLSVC) directly draining into the left atrium (LA). One patient had PLSVC and the partially unroofed terminal portion of the coronary sinus (CS), or sinus ostium open into LA. Another patient had total anomalous pulmonary vein connection and the partially unroofed mid-portion of CS without PLSVC. The operative methods of the unroofed coronary sinus included: ligating PLSVC and repairing ostium primum or secundum atrial septal defects in 4 cases; creating an intra-atrial tunnel from PLSVC to the right atrium (RA) and repairing the atrial septal defect in 2 cases; reconstructing the intra-atrial septal with a patch as a baffle to guide PLSVC or sinus ostium respectively towards RA in 2 cases; enlarging the defect on the coronary sinus roof and repairing the atrial septal defect of the coronary sinus type in one case. No patient died in this group. Because unroofed coronary sinus syndrome has atypical clinical manifestation and usually complicates varied congenital anomalies, its preoperative diagnosis is very difficult in some times and may draw the reliable support from echocardiography and catheterization. The operative program and method must depend not only upon its type but also upon PLSVC presence of absence, and communication between PLSVC and RSVC.

Adolescent↗
PubMed1996

[Analysis and identification of calculus equi components].

Components of the genuine calculus Equi and false Mabao were analyzed by X-ray diffraction. The results indicate that the main components of the genuine Mabo are NH4MgPO4.6H2O, NH4MgPO4.H2O and MgHPO4.3H2O, whereas the main components of the false Mabao is CaCO3.

Animals↗