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Biomedical subjects

W M Boyce

Publications and source records attributed to W M Boyce.

At least 37 records · Page 2Linked to original sources

Immobilization and physiological parameters associated with chemical restraint of wild pigs with Telazol and xylazine hydrochloride.

We used a combination of Telazol (3.3 mg/kg) and xylazine hydrochloride (1.6 mg/kg to immobilize 144 wild pigs (Sus scrofa) with blow darts. This drug combination was safe and effective for rapidly immobilizing animals ranging in size from 34 to > 170 kg and avoided difficulties associated with hand injections. For 123 single injection immobilizations, mean (+/- SD) induction times and effective handling periods averaged 5 (+/- 2.5) and 52 (+/- 18) min, respectively, and animals generally recovered for release within 120 min of initial injections. Animals that required two injections to immobilize (n = 21) received lower initial doses of Telazol and xylazine hydrochloride than those immobilized with a single injection because of errors in estimating body sizes; we found that there was a threshold dose required to immobilize wild pigs from 2.8 to 3.3 mg/kg Telazol and 1.4 to 1.6 mg/kg xylazine. Although neither age or sex influenced immobilization parameters, animals in good condition required longer to recover than those in poor condition. However, animals immobilized with two injections recovered as rapidly as those immobilized with a single injection. Heart rates and body temperatures declined slightly during the immobilization period, but respiration rates and blood oxygen saturation levels remained stable. In general, single injection immobilizations were preferable because they minimized problems associated with injecting partially immobilized animals. because it was difficult to accurately estimate the sizes of large wild pigs (> or = 90 kg), and because wild pigs that were partially immobilized were difficult to handle, we recommend increasing the drug doses to 4 mg/kg Telazol and 2 mg/kg xylazine hydrochloride when injecting relatively large animals to assure single injection immobilizations. Although recovery periods may be prolonged, higher doses of Telazol and xylazine should be safe based on data from domestic pigs.

Analysis of Variance↗

Pathogen exposure patterns among sympatric populations of bighorn sheep, mule deer and cattle.

We sampled sympatric bighorn sheep (Ovis canadensis, n = 31), mule deer (Odocoileus hemionus, n = 38), and domestic cattle (n = 26) in the San Bernadino Mountains of southern California (USA) for the presence of Psoroptes spp. mites and for serologic evidence of exposure to bluetongue virus (BTV) and Babesia spp. From 1991 through 1994, Psoroptes spp. infestations were found on 12 (44%) of 27 bighorn sheep. No mites were found on mule deer or cattle. The BTV serum antibody prevalence in a cohort of 26 cattle ranged from 17 to 89%. There was no evidence of exposure to BTV in the bighorn sheep or mule deer. The cumulative serum antibody prevalence of Babesia spp. during the study was 35% in 26 bighorn sheep and 85% in 20 mule deer, while antibodies were not detected in a cohort of cattle when they were sampled in May (n = 23) and December (n = 22) of 1992. Based on these results, we concluded that infestation with Psoroptes spp. and exposure to BTV was limited to bighorn sheep and cattle, respectively. In contrast, Babesia spp. infections appeared to be common in both mule deer and bighorn sheep while there was no evidence of exposure in cattle.

Animals↗

The distribution of Dermacentor hunteri and Anaplasma sp. in desert bighorn sheep (Ovis canadensis).

The ixodid tick Dermacentor hunteri has been collected intermittently this century, primarily from desert bighorn sheep (Ovis canadensis). Anaplasma spp. are intraerythrocytic rickettsial parasites of ungulates and are vectored in the western United States by ticks of the genus Dermacentor. We tested the hypotheses that D. hunteri would be found infesting all populations of desert bighorn, and that all infested populations would be seropositive for Anaplasma sp. Dermacentor hunteri was found on desert bighorn throughout their range in the Mojave and Sonoran deserts of the southwestern United States and northern Mexico, but not in any portion of the Chihuahuan desert of New Mexico and eastern Arizona or in Baja California Sur, Mexico. Using an indirect immunofluorescence antibody test (IIF), 8 populations of desert bighorn in California with D. hunteri were seropositive for Anaplasma sp. (n = 160). Four populations of desert bighorn with D. hunteri in Arizona (n = 69), 1 in Nevada (n = 22), and I in Utah (n = 14) with D. hunteri were seronegative. Six populations of desert bighorn were uninfested with D. hunteri and were also seronegative. Of these populations, 1 was in California (n = 19), 2 were in New Mexico (n = 33), 2 were in Utah (n = 30), and 1 was in Baja California Sur (n = 14). We found no support for either of our original hypotheses and concluded that both D. hunteri and Anaplasma sp. are limited in their distribution among desert bighorn. We also suggest a cautionary approach to translocations of desert bighorn given the high prevalence of ticks and the unknown effects of Anaplasma sp. on free-ranging bighorn.

Anaplasma↗

Fixation affects morphometric characters of Psoroptes cuniculi mites (Acari:Psoroptidae).

Thirteen morphologic characters were evaluated on Psoroptes cuniculi (Delafond) mites to determine if measurements for these variables were influenced by the use of different fixatives and varying periods of fixation. Morphologic structures composed primarily of chitin (outer opisthosomal setal lengths and right 3rd tarsal pedicle length) were not affected by either fixative type or duration of fixation. In contrast, alcohol and formalin fixation both resulted in significant reductions (P < 0.05) in mean body size measurements when compared with mites mounted in Hoyer's medium without prior fixation. The largest reduction in size was seen in mites fixed for the longest period, but there was no apparent association between size reduction and increasing concentration of alcohol or formalin. Surprisingly, when mites were remeasured 4 yr later, Hoyer's mounting medium was found to have had the opposite effect of fixation and caused an increase in body size. Mites mounted in Hoyer's medium without prior fixation had significantly increased body lengths, whereas mites mounted in Hoyer's following fixation in alcohol and formalin had significant increases in both body length and posterior body width. Our results indicate that fixation and mounting can significantly influence the morphometric analysis of mites and other arthropods. We recommend that morphometric studies be conducted using consistent methodology to reduce experimental bias, and these methodologies be reported in publications dealing with morphometric analyses.

Animals↗

Validity of using serological tests for diagnosis of diseases in wild animals.

Transposition of diagnostic tests used in domestic livestock species to free-ranging and captive wildlife species has two problems. First, most existing tests have not been adequately validated in domestic livestock. Second, assumptions that a serological test will perform identically in wildlife and livestock species may not be correct, due to differences in pathogenic strains and serovars, host serological responses, and exposure to organisms of similar antigenic structure which produce cross-reacting antibodies. Some assays require species-specific reagents/test components which might not be commercially available, and most assays have not been standardized. The authors outline the principles involved in the evaluation of a serological diagnostic test, and provide examples of how knowledge of test sensitivity and specificity can be used to estimate true prevalence, to determine whether a population is infected, and to facilitate management decisions with regard to animal translocations.

Animals↗

Seroprevalence of Psoroptes sp. mites in free-ranging elk (Cervus elaphus) as determined by kinetic ELISA.

Western blots and a kinetic enzyme-linked immunosorbent assay (ELISA) were used to characterize and quantify the prevalence of antibodies to Psoroptes sp. mites in elk (Cervus elaphus) from nine herds in North America. Sera from infested (n = 18) and non-infested (n = 22) elk were used to optimize test methodology and to define cut-off values for negative, suspect, and positive samples. Among 357 samples, 35 (9.8%) of the animals were seropositive, 259 (73%) were negative, and 63 (18%) were suspect. Six of nine herds (67%) contained positive animals and two additional herds (22%) had suspect animals. Sex was not associated with prevalence of antibodies, but adults greater than 2 yr old were approximately five times more likely (95% confidence interval = 2.6-15.4) to be seropositive than calves. Based on these results, we propose that exposure to Psoroptes sp. mites may be widespread in free-ranging elk of North America.

Animals↗

Pregnancy detection in bighorn sheep (Ovis canadensis) using a fecal-based enzyme immunoassay.

We developed and validated an enzyme immunoassay for immunoreactive pregnanediol-3-glucuronide (iPdG) in feces to monitor reproductive status in desert and Rocky Mountain bighorn sheep (Ovis canadensis). Fecal iPdG concentrations were strongly correlated (r = 0.71) with serum progesterone concentrations in paired fecal and blood samples collected from 34 free-ranging desert bighorn sheep. In bimonthly fecal samples collected from 12 captive ewes, fecal iPdG profiles were similar between desert and Rocky Mountain bighorn sheep and we selected a pregnancy detection cutoff value of iPdG > or = 1.8 ng/mg feces. Fecal iPdG concentrations always exceeded this cutoff value when samples were collected from about day 60 of pregnancy to a few days before parturition, but values < 1.8 ng/mg (false negatives) were common for samples collected during the first 60 days of gestation. Although we tested a small number of known pregnant and non-pregnant ewes, the accuracy of the assay was 100% when two samples, collected 2 wk apart, were evaluated for any given ewe. Based on these data, this direct enzyme immunoassay for fecal progesterone metabolites has promise as a diagnostic tool to monitor hormone excretion and pregnancy in a free-ranging ungulate species.

Animals↗

Antibody responses to Psoroptes sp. mites in Dall sheep (Ovis dalli).

We determined that antibody responses to Psoroptes sp. mites were not present in 403 of 407 sera samples collected opportunistically from 1979 through 1991 from Dall sheep (Ovis dalli) from five locations in Alaska, USA (Eastern Arctic, n = 61; Central Arctic, n = 15; Western Interior, n = 122; Central Interior, n = 63; Eastern Interior, n = 146). Test values for four samples exceeded the positive cutoff value for the immunoassay, but exposure to mites could not be confirmed since the 95% confidence interval for true prevalence ranged from 0 to 2.3%. Therefore, we concluded that these were probably false positive results. Our analysis, coupled with the lack of previous reports of mites or lesions in Dall sheep or other Alaskan ungulates, provided indirect evidence that Psoroptes sp. are not enzootic in Dall sheep in Alaska. In contrast, Psoroptes sp. have been reported in bighorn sheep (Ovis canadensis) and other wild ungulate populations from southern Canada to Mexico. These findings are compatible with the hypothesis that Psoroptes sp. were introduced into North America with imported domestic sheep and were not introduced by ancestral wild sheep.

Alaska↗

Seroprevalence of two Babesia spp. isolates in selected bighorn sheep (Ovis canadensis) and mule deer (Odocoileus hemionus) populations in California.

Sera from 111 bighorn sheep (Ovis canadensis) and 95 mule deer (Odocoileus hemionus) were tested using an indirect immunofluorescence assay for antibodies to two isolates of Babesia spp. recently obtained from these hosts in California (USA). The study populations were from six locations: three areas of real or potential sympatry of bighorn sheep and deer, one area with deer only, and two areas with bighorn sheep only. Antibody titers from seroreactive individuals were similar with both babesial isolate antigens (P < 0.05), and seroprevalence was highest in the areas of host sympatry. A moderate to high seroprevalence (> or = 30%) in some of the study populations was evidence that babesial parasites may be common in bighorn sheep and mule deer in some areas of California.

Animals↗

Geographic analysis of pathogen exposure in bighorn sheep (Ovis canadensis).

Antibody responses were examined among 998 bighorn sheep (Ovis canadensis) in California (USA) to determine spatial patterns of pathogen exposure. Using a shifting frame analysis, a specific geographic region was delineated that contained bighorn sheep with higher (P < 0.05) levels of multiple exposure (antibodies detected against > or = two pathogens), as well as higher prevalence values for eight of ten individual pathogens. This region in southwestern California encompassed all of the peninsular populations of bighorn sheep recently proposed for listing as endangered by the U.S. Fish and Wildlife Service.

Animals↗

Diffuse unilateral subacute neuroretinitis. Morphometric, serologic, and epidemiologic support for Baylisascaris as a causative agent.

PURPOSE: Several nematodes have been postulated as etiologic agents in diffuse unilateral subacute neuroretinitis (DUSN), but the cause of this condition remains uncertain. The authors report the first case of DUSN from the western United States (northern California), along with morphometric, serologic, and epidemiologic evidence supporting Baylisascaris procyonis as its cause. METHODS: One patient was examined and evaluated for disc edema and transient obscurations of vision. A diagnosis of DUSN was confirmed when a motile nematode was identified within the substance of the patient's retina. Morphometric analysis of the nematode was done from projected fundus photographs. Serologic evidence of Baylisascaris infection was suggested by Western blot analysis. A necropsy was done on 12 raccoons from the area. They were examined for evidence of Baylisascaris infection, previously believed to be nonendemic in the region. RESULTS: The intraocular nematode measured 1727 x 67 microns, most consistent with Baylisascaris. The patient had considerable exposure to raccoons, and was seropositive for B. procyonis infection on Western blot analysis. Necropsy evaluation showed B. procyonis infection in 8 of 12 raccoons examined from the area. CONCLUSIONS: The morphometric, serologic, and epidemiologic findings in this case provide evidence that the raccoon ascarid, B. procyonis, is a cause of the large nematode variant of DUSN.

Acute Disease↗

A retrospective serologic survey for Anaplasma spp. infection in three bighorn sheep (Ovis canadensis) populations in California.

Using an indirect immunofluorescence assay, we determined the prevalence of Anaplasma-reactive antibody in three herds of bighorn sheep, each a different subspecies and occupying a different habitat in California (USA). Antibodies to Anaplasma spp. were identified in none of twenty California bighorn (Ovis canadensis californiana) sampled from the Mt. Baxter herd, 11 of 17 peninsular bighorn (O. canadensis cremnobates) sampled in the Santa Rosa Mountains, and all 20 desert bighorn (O. canadensis nelsoni) sampled at Old Dad Peak/Kelso Mountains. Based on an assay and an adsorption technique, the titers most likely were due to Anaplasma ovis. The presence and species of tick vectors in each of the habitats, and the presence or absence of deer or livestock were identified as factors potentially influencing seroprevalence of antibodies.

Adsorption↗

Isolation and in vitro cultivation of Babesia parasites from free-ranging desert bighorn sheep (Ovis canadensis nelsoni) and mule deer (Odocoileus hemionus) in California.

Protozoal parasites of the genus Babesia were isolated for the first time from free-ranging desert bighorn sheep (Ovis canadensis nelsoni) and mule deer (Odocoileus hemionus) populations in California by in vitro culture of host blood. These naturally infected animals did not have microscopically detectable parasitemia at the time blood was collected for parasite cultivation. Three isolates of small Babesia parasites were cultured from different sample groups of bighorn sheep, and 2 isolates of large Babesia parasites were cultured from a group of bighorn sheep and a group of mule deer, respectively. The size and structure of the various forms of piroplasms from each isolate remained consistent throughout the period of cultivation. Statistical comparison of the sizes of the piroplasms among the isolates indicated that there were at least 2 distinct morphotypes. Four of the 5 isolates were maintained with continuous growth in cultures containing erythrocytes from uninfected donor bighorn sheep, mule deer, and domestic sheep. Cryopreservation or storage of cultures at 4 C for 7 days did not affect viability of the isolates. These results demonstrate the potential for use of in vitro cultivation methods for the isolation of Babesia parasites from free-ranging artiodactylids.

Animals↗

First isolation of Borrelia burgdorferi in southern California.

The Lyme disease spirochete, Borrelia burgdorferi Johnson, Schmid, Hyde, Steigerwalt & Brenner, was isolated from the blood of a dusky-footed wood rat, Neotoma fuscipes Baird, in the San Bernardino Mountains of southern California. Antigenic, protein, and molecular analyses demonstrated that the isolate varied slightly from most isolates of B. burgdorferi from northern California and was clearly distinct from other species of Borrelia that are endemic to the state. This is the first reported isolate of B. burgdorferi from southern California and demonstrates that the Lyme disease spirochete is enzootic in mountains near the major human population center of the state.

Animals↗

Exposure to Psoroptes sp. mites is common among bighorn sheep (Ovis canadensis) populations in California.

Sera (n = 806) from 50 populations of bighorn sheep (Ovis canadensis) in California (USA) were evaluated for antibodies to Psoroptes sp. mites using a kinetic enzyme-linked immunosorbent assay (ELISA). Test values for each sample were determined to be either positive or negative at each of two ELISA cutoff values that provided either 100% sensitivity (low cutoff) or 100% specificity (high cutoff), respectively. One hundred sixty-eight (20.8%) sera were seropositive at the low cutoff value, and 87 (10.8%) of these sera also were seropositive at the high cutoff value. Eleven populations were designated as scabies-suspect and 25 populations were designated as scabies-positive because they had at least one seropositive animal at the low and the high cutoff values, respectively. Based on these results, exposure to Psoroptes sp. mites appeared to be widely distributed among bighorn sheep populations from 1980 to 1990 and infested animals may have been present prior to 1980.

Animals↗

Serodiagnostic antibody responses to Psoroptes sp. infestations in bighorn sheep.

The antibody responses of bighorn sheep (Ovis canadensis) infected with Psoroptes sp. mites were investigated by enzyme linked immunosorbent assay on western blots of P. cuniculi antigens. Serum from 20 Psoroptes sp.-infested bighorn sheep (O. canadensis mexicana, O. canadensis nelsoni, O. canadensis canadensis) from New Mexico, Nevada, California, and Idaho reacted strongly with mite antigens ranging from 12 to 34 kd. Serum from 35 Psoroptes sp.-free bighorn sheep of unknown tick infestation status and from three Psoroptes sp.-free bighorn sheep infested with Dermacentor hunteri ticks did not react with these antigens. Psoroptes sp.-specific antibody responses were present throughout a 16 mo period in one infected bighorn sheep, but were not detectable 8 mo following successful treatment. These results demonstrate that specific serodiagnosis of Psoroptes sp. infestation is feasible in bighorn sheep and suggest that antibody responses are indicative of current or recent infestation.

Animals↗

Antigenic characterization of Psoroptes spp. (Acari: Psoroptidae) mites from different hosts.

Immunoblotting with defined antigens and antisera revealed extensive and nearly complete antigenic cross-reactivity between Psoroptes spp. mites from a bighorn sheep, a mule deer, a cow, and a rabbit. Antigenic differences were not detected between mites from the sympatric bighorn sheep and mule deer. However, minor antigenic differences between mites from the cow and rabbit suggested that these mites were distinct from each other, as well as from the mites from the bighorn sheep and mule deer. These results are consistent with earlier morphologic studies of these populations of mites and provide additional support for the hypothesis that putative populations and/or species of Psoroptes mites may not be reproductively or ecologically isolated, particularly when their hosts are sympatric.

Animals↗

Kinetic ELISA for detection of antibodies to Psoroptes sp. (Acari: Psoroptidae) in bighorn sheep (Ovis canadensis).

A kinetic enzyme-linked immunosorbent assay (ELISA) was developed using antigenic extracts prepared from Psoroptes cuniculi mites and sera from 37 Psoroptes sp.-infested and 43 uninfested bighorn sheep (Ovis canadensis). Serial dilutions of these serum samples, representing 3 bighorn sheep subspecies and 9 geographic areas, gave parallel responses when plotted as log dilution versus log kinetic rate. Therefore, all 80 samples were run at a single dilution (1:100) and positive/negative cutoff values were established as the mean kinetic rate of all negative sera plus either 2, 3, or 4 standard deviations. The resulting ELISA was highly reproducible and accurate with sensitivities and specificities of 100% and 97.7%, 94.6% and 97.7%, and 94.6% and 100%, respectively. This immunoassay will be useful for prospective and retrospective studies assessing the distribution and prevalence of Psoroptes sp. infestations in bighorn sheep.

Animals↗