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Biomedical subjects

W M Fischer

Publications and source records attributed to W M Fischer.

At least 19 recordsLinked to original sources
PubMed1992

GenBank.

The GenBank nucleotide sequence database now contains sequence data and associated annotation corresponding to 85,000,000 nucleotides in 67,000 entries from a total of 3,000 organisms. The input stream of data coming into the database is primarily as direct submissions from the scientific community on electronic media, with little or no data being keyboarded from the printed page by the databank staff. The data are maintained in a relational database management system and are made available in flatfile form through on-line access, and through various network and off-line computer-readable media. The data are also distributed in relational form through satellite copies at a number of institutions in the U.S. and elsewhere. In addition, GenBank provides the U.S. distribution center for the BIOSCI electronic bulletin board service.

Animals↗
PubMed1991

Fc receptor blocking antibodies after active immunization for the treatment of recurrent spontaneous abortion.

In a prospective study 140 couples who had at least three spontaneous abortions (RSA) were studied for the presence of Fc receptor blocking antibodies detected by the erythrocyte antibody rosette inhibition (EAI) assay, for anti-paternal cytotoxic antibodies (APCA), and for mixed lymphocyte culture inhibiting (MLCI) antibodies before and after active immunization with paternal lymphocytes. The comparative analysis revealed the EAI assay to possess a higher sensitivity than the APCA and/or MLCI tests in monitoring the specific immune response after active immunization. The success of pregnancy in EAI positive post-immunization patients was not influenced by the presence or absence of APCA or MLCI. In the light of a successful pregnancy outcome of 85.7% (n = 37) in this study we conclude that the monitoring of Fc receptor blocking antibodies is useful in active immunization protocols for RSA patients.

Abortion, Habitual↗
PubMed1990

Further characterization of the EAI factor induced by alloimmunization for treatment of recurrent abortion.

In order to remove the EAI "blocking" activity, EA inhibition positive sera from patients with recurrent abortions were absorbed after alloimmunization with paternal lymphocytes by the lymphoblastoid cell line (LCL) of the husband. To avoid non-specific binding via the Fc-receptor, the cells were first fixed with 0.05% glutaraldehyde. Absorption was performed for 3 h at 4 degrees C. For subsequent elution, the cells were incubated with 0.1 M glycine-HCl, pH 2.3, for 30 min at 4 degrees C. After each step, EAI assay was carried out to determine the "blocking" activity in the supernatants. Furthermore, 10% SDS-PAGE and immunoblotting with goat antihuman IgG of the whole serum and the supernatants were performed. The results obtained give evidence that the EAI "blocking" activity can be absorbed by and eluted from the LCL of the immunizing husband, and is due to an IgG antibody directed against an antigen present on the LCL. Further absorption experiments with trophoblast cells will show whether this antigen is also displayed by the trophoblast.

Abortion, Habitual↗
PubMed1979

A concept for stereological investigation of rat kidney.

An attempt is presented to adapt sampling procedures for stereological investigation of rat kidney. The proposed experimental design considers the zonal construction, in particular the structural and osmotic features of this organ. Every zone is treated as an own entity inregard to sample collection. In order to uncover possibly existing preferential orientation of structures the application of two mutually perpendicular section planes is recommended. Furthermore a method is described to determine absolute volumes and surface of structural components.

Animals↗
PubMed1976

[A suggestion for the evaluation of the antepartal cardiotocogram (author's transl)].

An evaluation scale for the antepartal cardiotocogram is suggested which is intentionally based on the Apgar scale. Five criteria were evaluated: three characteristics of the basal fetal heart rate and two characteristic, recognized alterations in heart rate. In regard to the interpretation of the basal heart rate, level, range and number of flat readings per minute are significant for the prognosis. Alterations in heart rate permit a prognostic statement depending on the form and type of the acceleration and deceleration 0-2 points are given for every characteristic so that the index can vary from 0 to 10 points. The condition of the fetus is physiologic when the index is between 8-10, the prognosis is questionable when the index is between 5 and 7 and the condition of the fetus is critical when it is 4 or lower.

Apgar Score↗