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Biomedical subjects

W M Hooker

Publications and source records attributed to W M Hooker.

9 recordsLinked to original sources
PubMed1975

Cation-dependent structures associated with membranes in the rat central nervous system.

As observed by high magnification electron microscopy, potassium ions induce osmiophilia at the extracellular side of triple layered plasma membranes in the central nervous system of the rat. In contrast, sodium ions induce a widening of the intracellular lamina of these membranes, and do not result in observable extracellular deposits. The observations suggest the existence of potassium and sodium ion-selective structures associated with opposite surfaces of the neuron membrane. Use of the fixative vehicle as a cytochemical reagent is proposed.

Animals↗
PubMed1975

Localization of the acetylcholine receptors in denervated muscles of rats.

ACh contractures of denervated lumbricalis muscles of rats were washed in Tris-methanesulfonate solutions and in sucrose solutions. The peak tension was diminished by about 80% following a 30 min exposure to solutions containing 400-600 mM glycerol when Tris solutions were used in the testing period, and by about 50% when sucrose solutions were used. The amplitude of the membrane potential changes provoked by ACh was decreased by about 36% following the glycerol treatment. The treatment had no effect on the ACh-induced 45Ca uptake of muscles. Electron microscopy of the glycerol-treated muscles showed widespread vacuolization apparently originating from swelling and disruption of the T system. It was concluded that ACh receptors which arise in the muscle membrane following nerve section are distributed in the external surface membrane and in the membranes of the T system.

Acetylcholine↗