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Biomedical subjects

W M Wong

Publications and source records attributed to W M Wong.

9 recordsLinked to original sources
PubMed1991

A widely distributed "CAT" family of repetitive DNA sequences.

The yeast genome contains a family of repetitive sequences consisting primarily of a tandemly arranged trinucleotide, CAT, or a closely related CGT sequence. To characterize similar sequences in divergent organisms, a previously isolated "CAT" sequence was used to isolate homologous genomic clones from a human cell line, an insect and a higher plant. Sequence analyses show that comparable repetitive sequences are widely distributed and may be present in all eukaryotic genomes. In situ hybridization analyses indicate that in yeast, the CAT elements are dispersed among all the chromosomes, and a more detailed analysis in Drosophila indicates that at least one of these sequences maps on the X chromosome between known genetic loci which are actively expressed. Repeated searches of yeast cDNA libraries indicate that these CAT clusters are not expressed but substantial effects on the expression of a cloned gene strongly suggest that they play an important role in gene regulation.

Animals↗
PubMed1990

Termination region in rRNA genes from a eucaryotic thermophile, Thermomyces lanuginosus.

S1 mapping of the termination region in the ribosomal DNA from a thermophilic fungus, Thermomyces lanuginosus, revealed three distinct termini corresponding to the mature 25S rRNA, a precursor that is 19 nucleotides longer and corresponds to the 37S precursor in yeast cells, and a putative termination site at +96 that bears a limited sequence homology with the SalI box of mammalian cells. An estimate of the secondary structure suggested that the three termini are in close proximity, a feature that may be essential to precursor termination and maturation. The results raise questions regarding recently reported relationships between ribosomal DNA termination and spacer enhancer elements in fungi.

Base Sequence↗
PubMed1990

Coronary risk profile screening during National Heart Week--1986.

Coronary risk profile screening was performed in 1422 volunteer subjects during National Heart Week 1986. A great proportion (55%) of this study group had blood cholesterol above 200 mg/dl; 10% of the subjects were smokers. This study documents the coronary risk profile of 1422 subjects whose biodata provide a baseline for future follow up of the possible changes in any of the various risk factors. This study identifies modifiable risk factors, high blood cholesterol and smoking which should be the target of public education in the efforts to reduce coronary diseases in Singapore.

Adult↗
PubMed1975

Ultrastructural deformation studies on biological membranes.

The ultrastructural deformation technique has been used to study various membranes, including several claimed to show subunit structure. With localized deformation fibers ca. 100-300 A in diameter and up to 4,000 A long were found extending across the cracks parallel to the draw direction in all membranes. Lipid extraction and proteolytic enzyme (papain) treatment of membranes has shown that the fibers are protein in nature. Deformation of membranes while still wet showed no significant change in the appearance of the fibers compared with those obtained from deformation of membranes after air drying, indicating that formation of fibers is not an artifact due to dehydration. There must be extensive interaction between the protein molecules and thus we indicate that the results do not agree with current suggestions that membranes are composed of individual protein subunits immersed in a lipid bilayer.

Animals↗