Fluorescein angiography of gingiva after experimental wounds.
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Biomedical subjects
Publications and source records attributed to W Mörmann.
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Attached gingiva's integrity was studied with the aid of fluorescein angiography and a standardized frenal tension test in 15 subjects aged between 19 and 41 years (9 female, 6 male) with mucogingival pathology in the region of the mandibular labial frenum. Each subject showed preoperatively gingival angiographic ischemia under 200 p frenal traction. The angiographic ischemia was in no case congruent to the clinical ischemia. One month after surgical enlargement of attached gingiva (eight grafts of palatal mucosa, seven subperiosteal vestibulum extension) both mucogingival surgery techniques successfully prevented the gingival blood circulation from showing ischemia clinically as well as angiographically under 200 p frenal traction.
"Free gingival grafts--a technique for obtaining grafts using the Mucotom": A new instrument with a mechanically oscillating blade for obtaining grafts from a palatal donor site was designed. The Mucotom can be attached to a dental unit. In 27 patients aged 18 to 46 the width of the attached gingiva in the mandibular anterior and bicuspid region was widened using for each case three independent grafts of ultrathin, thin and intermediate thickness. Each graft taken with the Mucotom was measured clinically in the center and at both ends using a Mitotoyo thickness measuring gauge. The average (+/- stand. dev.) thicknesses were in millimeters: 0.37 +/- 0.09 (M I), 0.55 +/- 0.12 (M II) and 0.75 +/- 0.14 (M II) respectively. Grafts obtained by manual scalpel preparation averaged 0.96 +/- 0.15 mm. Grafts of intermediate thickness (M III) showed excellent clinical healing of the donor and the recipient site. The precise cutting of the instrument and the direct visual control of the instrument's guidance produced grafts of uniform thicknesses and without microlacerations.
The ability of two different toothbrush bristle ends to produce traumatic gingival abrasion was assessed in a double blind study on 15 male and 15 female young adults. Brushing was performed in a circular fashion using a modified Bass technique. An apparatus allowing continuous visual feedback of the average brushing force permitted a degree of standardisation of the system. The upper left canine and bicuspid area had to be brushed for 30 seconds with cut toothbrush bristles (CP) and with round ended toothbrush bristles (RP) respectively. A two-week interval separated the two brushing sessions. Traumatic lesions of the attached gingiva were stained with a disclosing solution, photographed and evaluated planimetrically. The "cut bristles" caused gingival abrasions 30% greater in extent than the round end bristles. The difference was not due to single brushing strokes accidentally greater for the "cut bristles". The size of the lesions was not sex-dependent.
This study demonstrates changes in the gingival vascular supply of humans following various periodontal surgical procedures. A special system was used to photograph circulatory changes following an i.v. injection of sodium fluorescein to visualize blood supply. The results of this study suggest that the following concepts are important in the design of periodontal flaps. 1. Flaps should be broad enough at their base to include major gingival vessels. 2. A flap's length to width ratio should not exceed 2:1. 3. Minimal tension should be produced by suturing techniques and the tissue should be managed gently during the surgical procedure. 4. Partial thickness flap preparations to cover avascular areas should not be too thin so that more blood vessels are included in them. 5. The apical portion of periodontal flaps should be full thickness when possible.
"Free gingival grafts--a technique for obtaining transplant tissue using a newly developed Mucotom": A new instrument for obtaining palatal soft tissue grafts was designed. It has a mechanically oscillating blade. The Mucotom is driven by the dental unit like a handpiece. The attached gingiva in the mandibular anterior region was widened in over 100 cases through a simplified surgical procedure and by taking the grafts with the Mucotom. Intermediate split thickness grafts resulted in good esthetics and function. The superior cutting ability of the instrument produced smooth grafts of uniform thickness.
The surface quality of 2 composite filling materials (Adaptic with coarse hard quartz fillers and Cosmic with fine softer glass fillers) was evaluated using profilometry, scanning electron microscopy and light reflectometry after finishing with experimental diamond discs, 3M discs, corundum discs, polishing paste and also after sealing. Plaque formation on standardized surfaces of both composites was photographically recorded in 20 subjects. The sealed surfaces and the surfaces finished by the matrix alone were approximately 10 times less rough than after finishing procedures. Under in vivo conditions they reflected the most light, for both composites. The sealer failed to cover the whole composite surface. The unfinished and sealed surfaces lost their shine 3-7 days after placement in the mouth. Decrease in reflectance was independent of mechanical oral hygiene procedures. There were no significant differences in the roughness produced by the various discs on Adaptic surfaces. Diamond discs with particle size 1-3 micrometer resulted in least surface roughness and were the only instruments not destroying the filler particles of either composite. 3M discs and the rough corundum discs caused significantly more surface roughness of the Cosmic surface. Reflectometry showed that the finishing methods unfavourably affected composite surface smoothness. Among all finishing methods polishing paste produced the highest reflection value on the Cosmic surface. Adaptic surfaces were always associated with more plaque formation than Cosmic surface after all finishing methods.
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The capillary blood circulation of free gingival autografts during healing was studied by means of 45 fluorescein angiographs taken over a postoperative period of 14 days in 10 female patients, 21 to 36 years old. The surgical technique commonly used for transplanting gingiva was slightly modified in that no surgical dressings and, in 3 cases, not even sutures were employed to immobilize the grafts. During the first 3 postoperative days, fluorescent plasmatic diffusion of increasing intensity was observed. In one case, fluorescing capillary circulation was detected as early as 24 hours after surgery, fluorescent capillaries became evident in all graft sites and increased steadily in number. Between 9 and 14 days after surgery, an even capillary loop pattern was established.
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The average individual status of gingival inflammation was evaluated in two groups of 39 and 47 subjects by means of the Gingivitis Fluorescein Test (GFT) and the Sulcus Bleeding Index. The subjects of the test group received a prophylaxis and hygiene instructions which were effective in reducing the gingivitis after 12 days. However, the reduction was not paralleled by a simultaneous decrease of fluorescein recovered in mouthwashings. The oral hygiene of the control group's subjects was not altered and examinations were performed after 7 days. In both groups the average SBI-score did not exceed 1.2 in all examinations. The fluorescein content of the mouthwashings was approximately 10 times reduced when compared with previous findings. No correlation was found between the amounts of fluorescein in mouthwashings and the severity or extent of the clinically assessed gingival inflammation.
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