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Biomedical subjects

W Müller

Publications and source records attributed to W Müller.

At least 793 records · Page 44Linked to original sources
PubMed1980

Effect of phenformin on the metabolism of glucose, pyruvate and acetate in guinea-pig heart.

In the isolated perfused heart of the guinea-pig, phenformin could be shown to have a characteristic effect on cardiac performance and metabolism. In the working heart, phenformin (1 mmol1/1) decreased dp/dt and increased the end diatolic pressure; this reduced heart performance could not be explained by changes in the content of energy-rich compounds. Furthermore, phenformin increased lactate production in hearts perfused with glucose as substrate and inhibited the utilisation of pyruvate, but not of acetate. The activity of the purified pyruvate dehydrogenase complex was not influenced by phenformin, but the active form of the pyruvate dehydrogenase complex (PDHa) was diminished. This effect may be explained by an inhibition of the pyruvate dehydrogenase phosphatase. An inhibition of pyruvate dehydrogenase and, coincidently, of oxygen consumption might contribute to the development of lactic acidoses and, particularly, might be deleterious for the heart.

Adenine Nucleotides↗
PubMed1980

[Electron microscopic studies of reptilian lung innervation].

The Reptilian lung is richly innervated, in all species investigated bundles of nervefibres and autonomous ganglia have been detected. Several types of nerves and nerve endings of varicosities can be differentiated. The possible cholinergic, adrenergic and purinergic nature of the 3 types of nerve endings or varicosities is discussed on the basis of morphological criteria. The nerve terminals have been observed at intrapulmonary neurons and in the immediate neighbourhood of smooth muscle cells and of pneumocytes II.

Adrenergic Fibers↗
PubMed1980

[Ultrastructural studies of the alveolar epithelium of reptiles living in different habitats].

The alveolar epithelium of various reptiles living in different habitats has been investigated with the light and electron microscopes. In all species pneumocytes of type I and II can be distinguished. The comparatively uniform pneumocytes I cover the blood capillaries and usually exhibit a smooth surface. Occasionally they bear in their marginal parts, especially when bordering a pneumocyte II, some microvilli. Their main characteristics are: pinocytotic vesicles and apical bundles of microfilaments. In the aquatic Trionyx particularly thin areas resembling precursor structures of a fenestration have been detected. In this species the blood-air barrier is unusually thin (280...350 nm). The thickness of this barrier otherwise varies considerably: in Agama, Chamaeleo, Hemidactylus and Pseudemys it measures normally less than 400 nm. In Tamnophis, Caiman and various turtles it measures from 400 to over 500 nm. The pneumocytes II exhibit a remarkable morphological variability which concerns a) the cellular dimensions, b) the microvilli, c) diameter, number and structure of the lamellar bodies, d) the number of mucous granules, e) the lateral and basal labyrinth. The lamellar bodies are especially numerous in the terrestrial species Hemidactylus and Testudo. Most aquatic turtles contain few pneumocytes II with few lamellar bodies. In the squamata the blood capillaries bulge into the alveolar lumen, in turtles and tortoises they are deeply embedded into the connective tissue.

Animals↗
PubMed1979

Size fractionation of DNA fragments by liquid-liquid chromatography.

A method for the fractionation of double-stranded DNA fragments from 150 to 22000 b.p. in size by liquid-liquid chromatography is described. The procedure makes use of the fact that the partitioning of DNA in a polyethylene glycol-dextran system is size dependent and can be altered by alkali metal cations. Cellulose or celite are used as supports for the stationary, dextran-rich phase. Examples show the fractionation of digests of T7 DNA produced by Dpn II and Hind II restriction endonulceases as well as lambda DNA digests produced by Hind III and Eco RI restriction endonucleases.

Bacteriophage lambda↗
PubMed1979

[Diagnosis and clinical aspects of the cervicobrachial syndrome as seen by the rheumatologist (author's transl)].

Among the various diseases classified as cervicobrachial syndrome, from the rheumatological point of view rheumatic conditions of the soft parts such as periarthritis humeroscapularis, tendomyoses and insertion tendinoses are particularly important. Their exact clarification and differentiation from polymyalgia rheumatica and the rare polymyositis is also of great therapeutic significance. Moreover the rheumatologist is also frequently confronted with inflammatory and non-inflammatory conditions of the cervical vertebral column and the shoulder joint which may set up various disease symptoms. A whole series of affections come into consideration in the differential diagnosis and they are summarized in a table.

Arthritis↗
PubMed1979

Channelling of exogenous phenylalanine to the sites of storage and the sites of alkaloid and protein biosynthesis in Penicillium cyclopium.

Externally applied L-phenylalanine rapidly equilibrates with the cytosolic pool(s) in hyphae of emerged cultures of Penicillium cyclopium. If not incorporated into protein it is accumulated in the so called expandable pool, which is presumably localized in the vacuolar compartment. At high concentrations of exogenous L-phenylalanine practically all of the amino acid needed for protein synthesis comes from the extracellular source, contrary to alkaloid synthesis which under all conditions recruits more than 90% of the required L-phenylalanine from intracellular sources. Two pathways of alkaloid labelling can be distinguished, by which externally applied L-phenylalanine reaches the sites of alkaloid synthesis: (1) a direct way from cytosolic pool (primary labelling) and (2) an indirect way via the expandable pool (secondary labelling).

Alkaloids↗
PubMed1979

[Generalized periarticular calcinosis. Generalized hydroxylapatite disease].

The complex of symptoms know as generalized periarticular calcinosis is a disease observed clinically as occurring in periods lasting only a few days, and with a mainly monoarticular arthritic or tenosynovitis character which may be localized in various joints. A primary chronic course of the disease is rare. Crucial for the diagnosis is the demonstration of multiple periarticular calcifications, which can be identified biochemically as hydroxyapatite. The calcification probably initiates the burst of inflammation, and for this reason the symptoms can be certainly classified pathogenically as a crystalline synovitis. Etiologically, the disease is probably due to both genetic and acquired metabolic anomalies. Modification of calcification cannot be undertaken in our present ignorance of the exact pathogenesis, and for this reason the therapy of generalized periarthritis must remain symptomatic. The effect of non-steroid antiphlogistics, corticosteroids and colchicin is, however, unsatisfactory.

Adolescent↗
PubMed1979

[Visual field response to hypotension following oculopression (author's transl)].

The authors studied the visual fields in 100 healthy persons of different ages before and 10 min after oculopression. They found a depression of the I/2 isopter mostly in the nasal lower quarter of the visual field in persons over 60 years old, due to a disturbed or diminished vascular compensation of bulbus compression. They consider that oculopression might have clinical importance as an indirect provocation test of the cellular blood circulatory system.

Adolescent↗
PubMed1979

[Milk proteins for food. I. Some aspects of the use of milk proteins for the protein supply to the population of the GDR].

Milk protein accounts for approximately 21% of the protein supply to the population in the GDR. All milk protein fractions have a high nutritional-physiological value and favourable functional properties. This notwithstanding, only 50% of the milk protein processed by the dairy industry are at present employed in human nutrition, the remainder is used as a provender. The reasons for this situation are discussed. Principles of procedures for the industrial production of milk protein concentrates are presented.

Amino Acids↗
PubMed1979

[Determination of trace elements in dietary fats and emulsifiers by non-flame atomic absorption spectrometry (AAS). 2. Determination of mercury in dietary fats and emulsifiers].

Of the trace elements, mercury holds an exceptional position as to its chemico-physical and toxic properties. In the light of the special analytical problem, the methods used (cold-steam AAS in combination with the addition method) are discussed. The experimental conditions required for the digestion and the cold-steam AAS of samples of emulsifiers and dietary fats are indicated. The accuracy and the reproducibility of the procedure are dealt with, and the amounts of mercury found in products of the oil and margarine industries are reported.

Dietary Fats↗
PubMed1979

NADH-dependent reduction of D-proline in Clostridium sticklandii. Reconstitution from three fractions containing NADH dehydrogenase, D-proline reductase, and a third protein factor.

The enzyme system from Clostridium sticklandii catalyzing the NADH-dependent reduction of D-proline was co-purified by chromatography on DEAE-cellulose at pH 8.2 and ammonium sulfate fractionation, and resolved into fractions containing three different protein components, NADH dehydrogenase, D-proline reductase and a third protein factor, by chromatography on DEAE-cellulose at pH 7.0. Upon recombination of the fractions containing the three different protein components, the NADH-dependent reduction of D-proline was successfully reconstituted. The NADH dehydrogenase fractions oxidized NADH in the presence of artificial electron acceptors, and were inhibited by p-hydroxymercuriphenylsulfonate (50% at 80 nM). They contained 3--4 different enzyme bands as revealed by polyacrylamide-gel electropherograms stained with the NADH-dependent reduction of 2,3,5-triphenyltetrazolium chloride. D-Proline reduction was also coupled to a leuco-methylene blue-generating system containing D-glucose and glucose-oxidase (EC 1.1.3.4). Circumstantial evidence indicated that, among the clostridial proteins, only D-proline reductase and the third protein factor were needed for this reaction.

Bacterial Proteins↗
PubMed1979

Iron-induced atypical meningioma cells?

Polymorphous tumor cells with bizarre giant nuclei are characteristic of the so-called atypical meningioma. Some of these cells are iron-positive. Meningioma cells apparently are able to store iron excessively. It is discussed whether an extremely high intracellular iron uptake is responsible for atypical meningioma growth. Derangement of mitotic spindles may lead to polyploid chromosome sets and thereby to giant nuclei. This seems likely because induction of tumor growth after iron injection has been described. Spodographic examinations showed that even histologically inconspicuous tumor cells contain fine-grained iron. Application of the Prussian-blue reaction to spodograms reveals distinctly higher iron contents than common paraffin sections had proved.

Cell Nucleus↗