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Biomedical subjects

W Marx

Publications and source records attributed to W Marx.

At least 37 records · Page 2Linked to original sources
PubMed1989

Sufentanil, alfentanil, and fentanyl: impact on cerebrospinal fluid pressure in patients with brain tumors.

In order to evaluate the safety of the new synthetic opioids, alfentanil and sufentanil, in neurosurgical patients, we administered sufentanil 1 microg/kg i.v., alfentanil 50 microg/kg i.v. followed by an infusion of 1 microg/kg/min, or fentanyl 5 microg/kg i.v. to 30 patients with supratentorial tumors anesthetized with nitrous oxide (N2O), 60% in O2. Lumbar cerebrospinal fluid pressure (CSFP) and mean arterial pressure (MAP) responses were recorded for 10 min thereafter, while ventilation was held constant [mean PaCO2 = 36.1 +/- 1.0 mm Hg (SEM)]. There was no change in CSFP after fentanyl. In contrast, both sufentanil and alfentanil caused increases in CSFP, equal to 89 +/- 31 % SE (p < 0.05) and 22 +/- 5% (p < 0.05), respectively. MAP decreased after administration of each opioid. Peak decreases in cerebral perfusion pressure (MAP - CSFP) were 14 +/- 3% after fentanyl, 25 +/- 5% after sufentanil, and 37 +/- 3% after alfentanil. It is concluded that because sufentanil increased CSFP in patients who have brain tumors, it also may be contraindicated in other neurosurgical patients at risk for intracranial hypertension. Alfentanil may share this propensity, since CSFP increased despite a profound reduction in MAP. Among the three opioids evaluated, only fentanyl appears to be appropriate for supplementing N2O-2 anesthesia in patients who have compromised intracranial compliance.

Journal Article↗
PubMed1985

Automated determination of organic sulfur compounds. I. Spectrophotometric measurement of inorganic sulfate by low-pressure exchange chromatography.

A method for determination of inorganic sulfate is described which represents the terminal step of an automated assay of organic sulfur compounds in biological fluids. Nanomole quantities of inorganic sulfate were applied to a barium chloranilate column. Corresponding amounts of chloranilate ion, released as a result of an exchange reaction, were then measured by uv absorption at 313 nm. The reproducibility and sensitivity of the method and a calibration curve are reported.

Ammonium Sulfate↗
PubMed1980

Glycosaminoglycans of some mouse mastocytomas.

The glycosaminoglycan fractions of four murine mastocytomas (AB-CBF1-MCT-1, AB-CXBG-MCT-1, AB-CXBI-MCT-1, and Furth), were isolated and characterized using microelectrophoresis, degradation with specific mucopolysaccharidases, paper chromatography and, in some instances, ion-exchange chromatography. Intramuscular and ascites tumors and tumors cultured in vitro were used. The glycosaminoglycans were labeled with [35S]sulfate and , in a few instances, with D-[6-3H]glucosamine. The glycosaminoglycan fraction obtained from the AB-CBF1-MCT-1 mastocytoma cultured in vitro consisted predominantly of dermatan sulfate-like material. On the other hand, the major components of the glycosaminoglycan fractions of the AB-CXBG-MCT-1 and AB-CXBI-MCT-1 and Furth tumors were observed to behave like heparin or heparan sulfate. In most of the mastocytoma samples examined, chondroitin 4-sulfate-like components were found to be present in smaller amounts, even in samples derived from tumor cells cultured in vitro. A component exhibiting a keratan sulfate-like behavior was detected in fractions obtained from the solid CXBG, CXBI and Furth tumors.

Animals↗
PubMed1980

[Morbidity studies, following continuous electronic and biochemical monitoring of 1,000 childbirths (author's transl)].

A group of 1,000 electronically and biochemically monitored children of high-risk deliveries was examined for morbidity up to the age of one year.--Transfer to the Paediatric Hospital of Jena University had been necessary for 147 newborns for low weight and/or impaired adaptation. Sixteen of 18 neurologically conspicuous children, which had temporarily displayed electro-encephalographic or echo-encephalographic pathological or marginal findings, did no longer exhibit any neurological deficiency or signs of impaired intelligence, when re-examined after ten months. Only two children had severe disorders in terms of infantile cerebral paresis, one of them with a genetically determined disease (Morbus Langdon Down).--Incidence of infantile cerebral paresis dropped from more than three to something between one and two per cent owing the antepartum, intrapartum, and postpartum intensive monitoring as well as to intensive neonatal care. It is assumed that this will act to decrease the number of children with mental retardation.

Adult↗
PubMed1975

Proteoglycans of soluble fraction of mouse mastocytoma.

Proteoglycans have been isolated from a high speed supernatant fraction of a mouse mastocytoma by procedures which should minimize alteration of the native protein-polysaccharide molecule. The methods used include in vivo labeling proteoglycans with 35S-sulfate, 3H-leucine and 3H-lysine, centrifugation of the tumor homogenate at 105,000 g, cetylpyridinium fractionation of the supernatant, and further purification of some of the fractions obtained by DEAE-cellulose column chromatography, gel filtration on Sepharose 4B and cellulose acetate electrophoresis. Two major sulfated proteoglycans were obtained, one containing keratan sulfate-like material (KSP-S), the other a heparin-like polymer (HP-S). The presence in HP-S of a compound similar to heparin was confirmed by its digestibility with flavobacterium heparinase. HP-S contained about 4 per cent protein. Glycine was the predominant amino acid, and serine did not appear to be involved in the peptide-carbohydrate linkage. The proteoglycan present in HP-S appeared to be homogeneous when examined using cellulose acetate electrophoresis. KSP-S was found to contain sialic acid and its protein content was significantly higher than that of HP-S. Glutamic and aspartic acids were the most abundant amino acids in KSP-S.

Amino Acids↗