[Enzymes of the glyoxylase system in placenta samples from normal pregnancy].
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Biomedical subjects
Publications and source records attributed to W Matuszewski.
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Glyoxalase I and glyoxalase II (EC. 4.4.1.5 and EC 3.1.2.6) were separated by gel filtration on Sephadex G-75 and G-100. This simple procedure permitted also the partial purification of glyoxalase II. The purification coefficient in a single run from supernatant from beef liver was about 1 : 30 compared with 1 : 15 after the fifth step of purification with classical methods.
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Methods for determining allantoin in biological, cosmetic, and pharmaceutical samples are reviewed. The methods cover 3 main categories: spectrophotometry, alkalimetric titration, and chromatography (thin-layer and liquid). Comments are given on suitability for different sample types and on further development of methods for allantoin determination.
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