Biomedical subjects

W Minder

Publications and source records attributed to W Minder.

9 recordsLinked to original sources

PubMed1983

Isoelectric focusing of serum alkaline phosphatase isoenzymes.

We present a new method for the separation of alkaline phosphatase isoenzymes based on isoelectric focusing of serum proteins. Sera of healthy subjects studied by this method present seven groups of bands within a pH range of 3-9. To date, the organ source of six out of seven groups has been identified directly or indirectly. Serum of patients with histologically proven malignant neoplasms showed an additional group in the pH range 3.6-4.2. This fraction seems to be heterogeneous with respect to the pattern and stability to heat, and to L-phenylalanine and L-homoarginine inhibition. It was present in all but two of the proven malignant tumour patients but also in 26 out of 70 with benign disorders.

Alkaline Phosphatase↗

PubMed1982

Quality of breast milk: its control and preservation.

The numerous problems of bacterial contamination and proper preservation of breast milk have been examined. Both the many advantages of human milk as the optimal nourishment for the newborn baby and the extensive experiences of veterinary medicine in the handling of milk have been carefully considered. Bacterial multiplication has been studied under various storage conditions. Out of several possible procedures a simple, inexpensive, rapid and reliable dip-slide screening method for determining total bacterial colony counts is described. A clear-cut procedure for the optimal collection of breast milk is presented.

Humans↗

PubMed1980

[Determination of urea in blood and serum by the reflotest-urea method (author's transl)].

Tests in five laboratories on pooled sera in three concentration ranges demonstrated good reproducibility of urea concentration with the Reflotest-Urea, at a variation coefficient from 2.4% to 5.0%. Comparison with the urease/GLDH, Berthelot and diacetylmonoxim tests on sera from routine samples indicated good agreement of results even for pathological samples. Using whole blood there was also good agreement with results with plasma of the same samples. The Reflotest-Urea method is simple because different dose ranges can be used and the reaction time of ten minutes is not crucial. In-vitro studies using 37 of the most commonly used drugs in different areas of medicine failed to reveal any interference with the test.

Analysis of Variance↗

PubMed1979

[Determination of thyroxine in serum by a heterogeneous enzyme immunoassay: results of a joint trial].

This paper describes the evaluation of a heterologous enzyme immunoassay for the determination of total thyroxine in serum by a group of seven clinical chemical laboratories. The test follows the principles of the enzyme linked immunosorbent assay (ELISA) and uses peroxidase as a marker. The evaluation of analytical reliability yielded the following results within the analytical range from 39 unto 322 nmol/l: 1. Within-batch precision ranged from 3.1 unto 10.4% (coefficient of variation) with single analyses. 2. Between-batch precision ranged from 3.7 unto 20.4% with single analyses. 3. Between-laboratories precision ranged from 5.4 unto 6.8%. 4. Pure thyroxine, added to serum or thyroxine-free serum, gave recoveries between 93 and 120%. 5. Analysis of control sera gave results essentially comparable to the assigned values based upon radioimmunoassays. 6. Analysis of 288 clinical sera gave slightly higher results by the enzyme immunoassay than by the analogous radioimmunoassay from the same manufacturer. 7. Comparison with other methods of analysis (radioimmunoassays, competitive protein ligand assays, hormonal iodine assay) yielded partly comparable, partly higher results. 8. Comparison with the homogenous enzyme immunoassay (EMIT) led to comparable results. 9. Interference due to hyperlipemia or hemolysis was not observed. 10. There might be an interference in hyperbilirubinaemic sera, due to an as yet unknown factor. With respect to practicability the ELISA-test compares favourably with the analogous solid phase radioimmunoassay. The main differences are the absence of radioactive material and a longer shelf-live of reagents. Following the manual procedure the time taken to perform the enzyme immunoassay is slightly longer than for the analogous radioimmunoassay.

Enzyme-Linked Immunosorbent Assay↗

PubMed1978