Biomedical subjects

W Mingrone

Publications and source records attributed to W Mingrone.

4 recordsLinked to original sources

PubMed2001

Thalidomide in multiple myeloma, myelodysplastic syndromes and histiocytosis. Analysis of clinical results and of surrogate angiogenesis markers.

BACKGROUND: Thalidomide, as a single agent, has been recently found to induce a clinical response in one third of refractory or relapsed myeloma patients. Although it has been reported that thalidomide significantly inhibits angiogenesis. it is still unclear whether its clinical effect is mediated, at least in part, by its anti-angiogenic properties. PATIENTS AND METHODS: We evaluated thalidomide as a single agent in myeloma, myelodysplastic syndromes (MDS) and histiocytosis, i.e. hematological diseases characterized by increased angiogenesis, and measured prospectively a number of surrogate angiogenesis markers. RESULTS: Clinical responses were observed in 7 of 17 myeloma and 2 of 5 MDS patients. The histiocytosis patient had a partial response. At the time of the best clinical response, plasma levels of angiogenic growth factors, vascular endothelial growth factor (VEGF) and basic-fibroblast growth factor (b-FGF), were significantly decreased, and flow cytometry indicated a decrease of activated endothelial cells in the bone marrow of responding MDS patients. CONCLUSIONS: These observations confirm thalidomide efficacy in myeloma, suggest a possible use in MDS and histiocytosis and may contribute to the prediction of clinical response and to understanding the mechanism of thalidomide's action.

Aged↗

PubMed2000

Prognostic models for diffuse large B-cell lymphoma.

Prognosis of DLCL patients is variable and associated with well-defined risk factors. In the past decade several pretreatment variables have been incorporated into prognostic models to predict the death risk of individual patients. The International Prognostic Index (IPI), developed in an international consensus study, has been one of the most widely accepted of these models. In our study we applied some of the major prognostic models proposed for DLCLs in a cohort of 111 patients uniformly treated with a CHOP-like regimen in order to compare their sensitivity and specificity. We also evaluated the possibility of improving the IPI with the inclusion, from among the variables analysed, of serum beta-2 microglobulin level (beta-2M). The sensitivity, reflecting the ability to predict all failures in the cohort of patients as a whole, has improved from 45 to 73 per cent when the beta-2M-IPI model is compared with IPI, without a significant loss of specificity. Based on these results, the beta-2M-IPI may be useful for identifying the subset of patients with very poor prognoses. Therefore, the use of the serum beta-2M value in addition to the IPI may help in selection of the patients with DLCL at higher risk for treatment failure, and identification of those who may require specifically tailored therapeutic approaches.

Adult↗

PubMed2000

Molecular detection of circulating neoplastic cells in patients with clinically localised gastric and non-gastric mucosa-associated lymphoid tissue lymphoma.

BACKGROUND: Unlike other low-grade lymphomas, extranodal marginal zone B-cell lymphoma of mucosa-associated lymphoid tissue type usually presents with localised disease. AIM: To detect peripheral blood lymphoma involvement to establish the incidence of occult lymphoma dissemination. PATIENTS AND METHODS: In a series of 18 cases, peripheral blood was analysed by polymerase chain reaction, with primers directed to the third-complementarity determining region of the immunoglobulin heavy chain gene. RESULTS AND CONCLUSION: The presence of circulating neoplastic cells was detected in 21% of clinically localised cases. Moreover lymphoma cells were detected in 2 out of 6 morphologically normal bone marrow specimens. The present data show that, combining morphological and molecular methods, occult dissemination can be found in a large proportion of cases thus stressing the need for careful staging procedures. However, it has still to be clarified whether the presence of polymerase chain reaction-detectable circulating lymphoma cells can influence the outcome of mucosa-associated lymphoid tissue lymphoma patients submitted to antibiotic treatment (for gastric localisation) or local therapy (surgery or radiation, for non-gastric tumours).

Adult↗

PubMed1995

Mapping event-related brain potential microstates to sentence endings.

We analyzed topography and strength of 20 channel event-related potential maps to sentence endings differing in correctness, verbal vs. nonverbal surface form, priming, and repetition count. Seventeen healthy subjects silently read correct and incorrect versions of simple sentences with predictable color endings, and of more complex sentences with predictable composite word endings. Color endings appeared in verbal and nonverbal form. Measures of map topography (centroids of the positive and negative areas of the average referenced maps) and strength (Global Field Power) were analyzed. Adaptive segmentation distinguished a pre-N400 and a N400 microstate in the N400 time range. Topography differed between these two microstates, between verbal and nonverbal endings, and between correct color, incorrect color, and incorrect noncolor words. All verbal endings evoked left-laterlized negativity and right lateralized positivity in the pre-N400 microstates. Correct verbal endings evoked consistent posterior postivity and anterior negativity with left-lateralized gradient strength suggesting language-specific processing. New, incorrect noncolor words evoked reversed anterior-posterior N400 and pre-N400 map topographies with more anterior positivity and more posterior negativity than correct colors in each subject. Gradient strength and current source density maps also differed from those to correct colors. Strongest gradients were left-posterior in the pre-N400 but anterior in the N400 microstate, consistent with anterior activity contributing to the posterior N400 negativity. Incorrect and correct colors, which were semantically primed and repeated, showed smaller topographic differences and N400 effects with a different topography. These different maps can not arise by modulation of a single pattern of neural activity and show that the N400 time range consists of multiple distinct microstates.

Adult↗