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W Mol

Publications and source records attributed to W Mol.

7 recordsLinked to original sources
PubMed2004

A new technique of microvascular suturing: the chopstick rest technique.

With the conventional techniques of tying knots during microvascular anastomosis or neural suturing, time may be lost due to various reasons. The loose end of the suture often falls down into the operative field and gets stuck to the surrounding tissues. In the process of retrieving the suture, the surrounding tissues can be picked up together with the suture. When the posterior wall technique [Br J Plast Surg 34 (1981) 47, Plast Reconstr Surg 69 (1982) 139, Microsurgery 8 (1987) 22, J Reconstr Microsurg 15 (1999) 321] is used, the loose end of the suture may be stuck to the backside of the vessel and may be hard to grab. In order to avoid those problems, a new way of tying a microsuture was developed. By avoiding contact of the loose end of the suture to the surrounding tissue at any point during tying, the microvascular anastomosis can be performed quicker and more efficiently.

Humans↗
PubMed1994

PCR-mediated genotyping of Candida albicans strains from bone marrow transplant patients.

DNA amplification using polymerase chain reaction (PCR) primers aiming at eukaryotic or prokaryotic repetitive DNA motifs enables discrimination between individual Candida albicans isolates. This PCR-mediated DNA fingerprinting procedure was used to monitor yeast colonisation in immune-compromised leukaemia patients (n = 11) who were undergoing bone marrow transplantation (BMT). The leukaemia patients remained colonised by the same strain throughout a 5 month study period, irrespective of intermediate treatment with fungostatics or application of BMT-related therapies. All 11 strains could be identified separately by PCR fingerprinting. This implies that spreading of C. albicans from patient to patient does not seem to occur in this study group, despite the fact that medical employees frequently travel between wards which are in close proximity to other departments harbouring neutropenic patients. Several of the patients (n = 35) were also monitored for C. albicans colonisation and strain typing corroborated the lack of extensive cross-contamination during the study period. The application of PCR-mediated genotyping of fungi in epidemiological analyses and evaluation of hospital hygiene is discussed.

Base Sequence↗