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W N Ross

Publications and source records attributed to W N Ross.

44 records · Page 3Linked to original sources
PubMed1979

Species-specific effects on the optical signals of voltage-sensitive dyes.

The absorption changes of two merocyanine dyes in response to membrane potential changes were measured on several neuronal preparations to see whether the dyes would be useful in recording from these cells. We were able to record large signals without averaging from barnacle and leech neurons. The greatest signal with WW375 was seen at 750 nm. Much smaller increases in transmitted light intensity were seen at all other wavelengths between 500 and 780 nm. In contrast, vertebrate neuronal preparations produced much smaller signals with an entirely different action spectrum. Essentially the same spectrum was seen in cells of the sympathetic ganglion of the bullfrog, Rana catesbiana, dissociated chick spinal cord neurons, or dissociated rat superior cervical ganglion neurons. In each case an action potential was accompanied by increases in transmitted light intensity between 500 and 600 nm and 730 and 780 nm, and decreases in intensity between 600 and 730 nm with the dye WW375, the best dye tested. Similar results were obtained with dye NK2367 on both vertebrate and invertebrate preparations, except that the spectral properties were shifted 30 nm towards the blue. Both dyes caused some photodynamic damage to the cultured neurons after a few minute's exposure to the illuminating light. Several analogues of these dyes were also tried, but did not produce larger signals.

Action Potentials↗
PubMed1978

Voltage sensitive calcium channels in the presynaptic terminals of a decrementally conducting photoreceptor.

1. Intracellular recordings were made from the presynaptic regions of the photoreceptors of the median ocellus of the giant barnacle, Balanus nubilus.2. Millivolt changes in membrane potential near the dark resting level in the terminals elicit post-synaptic activity and consequently must be sufficient to modulate transmitter release from these endings.3. In normal saline the terminal voltage usually changes in a graded manner to increasing intensities of illumination of the cell. When the terminal region is superfused with saline containing TEA, 3-AP or high concentrations of K, an all-or-none action potential can be elicited consistently by light or injected current.4. The peak value of this action potential depends on the Ca concentration in the saline. The action potential can be generated if Sr or Ba ions replace Ca, but is reduced or blocked if Mg, Co, or Mn ions are added to the saline. It is virtually unaffected by TTX or replacement of Na with TMA ions in the saline. These results suggest that Ca carries most or all of the inward current during the action potential.5. The action potential is followed by a large undershoot which can last several seconds. The amplitude and duration of the action potential and the duration of the undershoot all grow in increasing concentrations of TEA up to 400 mM, the highest concentration tested. The threshold for the action potential decreases as the concentration of TEA is increased to 10 mM; increasing the concentration further has no effect on the threshold. These observations suggest that TEA blocks a voltage-sensitive potassium conductance at low concentrations but has less effect on the current responsible for the undershoot.6. Electrophysiological and pharmacological evidence suggests that the Ca channels are concentrated in the presynaptic terminals of this photoreceptor.

Action Potentials↗
PubMed1977

Changes in absorption, fluorescence, dichroism, and Birefringence in stained giant axons: : optical measurement of membrane potential.

The absorption, fluorescence, dichroism, and birefringence of stained squid axons were measured during action potentials and voltage clamp steps in an effort to find large optical signals that could be used to monitor membrane potential. Changes in all four optical properties were found that were linearly related to membrane potential and, with several new dyes, the signal-to-noise ratios were larger than any obtained previously. The problem of photodynamic damage was greatly diminished; with a merocyaninerhodanine dye, the photodynamic damage associated with intense light and the presence of oxygen was negligible. The absorption change obtained with this dye was relatively large; it could be measured with a signal-to-noise ratio of 100:1 during a single action potential.

Action Potentials↗
PubMedDate not supplied

Localized Ca2+ and calcium-activated potassium conductances in terminals of a barnacle photoreceptor.

Calcium channels are found in the presynaptic terminals of neurones, where they have a key role in synaptic transmission. They are also found in the somata of many cells, in dendrites and along a few axons. In no cell is the actual distribution of these channels known in detail, because there are no known toxins or other agents suitable for labelling calcium channels, and the current through these channels is usually too small to be quantified with extracellular electrodes. However, several experiments have suggested that the density of the channels is less in the axon than in the cell body or terminal region. Here we have used the indicator dye Arsenazo III in conjunction with an array of photodetectors to examine the spatial influx of calcium in the presynaptic terminal region of the giant barnacle, Balanus nubilus. In these cells, calcium entry occurs in a restricted region less than 50 micron in length, which corresponds closely to the region of synaptic contact with second-order cells. Outside this area the magnitude of calcium entry is reduced at least 50-fold. With reasonable assumptions it follows that the calcium channel density is equally localized. In addition, we demonstrate that these cells have a calcium-activated potassium conductance. Since calcium entry is restricted to the synaptic zone, this conductance must be effective only in this region.

Action Potentials↗