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W P ROWE

Publications and source records attributed to W P ROWE.

At least 19 recordsLinked to original sources
PubMed1965

A STUDY OF THE ANTIGENS INVOLVED IN ADENOVIRUS 12 TUMORIGENESIS BY IMMUNODIFFUSION TECHNIQUES.

The use of the agar gel diffusion technique has established the presence of three distinct antigenic reactions in the sera of Ad. 12 tumor-bearing hamsters. Only one of these antigens is directly demonstrable in the tumor. This "tumor" antigen is also formed during early stages of the infectious cycle in tissue culture cells. Other antigens present in the tumor, but only demonstrable indirectly with the use of antibody-containing serum of tumored hamsters, are the classical type-specific C antigen, and a new antigen, termed D. Of ninety-eight Ad. 12 tumored hamster sera, six reacted in gel diffusion with virus and tumor preparations, and thirty-one with tumor only. Sera which reacted in gel diffusion with viral antigen uniformly bad neutralizing antibody and high titers of CF antibody against viral and tumor antigens; however, many sera with comparable antibody titers did not react with the virus in gel diffusion. Sera which reacted in gel diffusion only with tumor antigen also had high CF antibody titers, but there was no correlation with neutralizing antibody.

Adenoviridae↗
PubMed1964

ENZOOTIC SENDAI VIRUS INFECTIONS IN MOUSE BREEDER COLONIES WITHIN THE UNITED STATES.

Naturally occurring enzootic infections of Sendai virus have been detected by serologic monitoring and virus isolation in mouse breeder colonies from five states in the United States of America. Six of 21 colonies tested were found to be infected with Sendai virus, and the mean incidence of infection within infected colonies was 76 percent. Forty-one of 120 attempts to isolate the virus were successful.

Animals↗
PubMed1964

IMMUNOFLUORESCENT STUDIES OF THE HISTOPATHOGENESIS OF LYMPHOCYTIC CHORIOMENINGITIS VIRUS INFECTION.

LCM virus antigen was visualized in infected tissue cultures and mouse tissues by the direct immunofluorescent procedure. In all instances, antigen was localized to the cytoplasm. In intracerebrally infected mice, antigen was almost completely restricted to meninges, choroid plexus, and ependyma; at no time were neurons involved. Mice infected by intraperitoneal inoculation of a viscerotropic strain demonstrated antigen chiefly in liver parenchyma, splenic reticulum, bronchi, and alveolar cells. Congenitally infected mice showed antigen in almost all cell types observed, but generally in only a minority of cells; infection was often focal in distribution. Liver, kidney, pregnant uterus, and trophoblast were most extensively involved. No antigen was observable in ova of a pregnant mouse.

Animals↗
PubMed1964

IMMUNOFLUORESCENT STUDIES OF ADENOVIRUS 12 TUMORS AND OF CELLS TRANSFORMED OR INFECTED BY ADENOVIRUSES.

Complement-fixing (CF) antibody-positive sera from hamsters bearing adenovirus type 12 (Ad. 12)-induced tumors revealed specific immunofluorescent stainable antigens in essentially all Ad. 12 hamster tumor cells. The antigens were primarily in the form of cytoplasmic flecks; less frequent staining was seen as nuclear flecks or homogeneous staining of nucleus and cytoplasm of a small proportion of cells. Tumor cells did not stain with rabbit antisera to crude Ad. 12 virus or A and C antigens. The hamster serum also stained cytoplasmic flecks in an Ad. 12-induced BALB/c mouse tumor and Ad. 12-"transformed" hamster embryo tissue culture cells. The hamster serum also stained fleck-shaped antigens in hamster and human cell cultures inoculated with homologous and heterologous adenovirus types, although the hamster cells did not react with the rabbit Ad. 12 antiserum. Attempts to identify the fluorescent-stainable fleck-shaped antigens indicated that they are not previously recognized viral antigens and that the cytoplasmic antigens formed in hamster cell cultures inoculated with Ad. 12 are different from those in tumors and in acutely infected human cell cultures.

Adenoviridae↗
PubMed1964

DETECTION OF SPECIFIC ANTIGEN IN SV40-TRANSFORMED CELLS BY IMMUNOFLUORESCENCE.

With an immunofluorescent technique involving the use of serum of hamsters with SV40 tumors, nuclear fluorescence was detected in each of five cell lines, derived from four mammalian species, transformed by SV40 virus. Essentially all nuclei, including those of multinuclear cells, were fluorescent-stainable. Serum of hamsters bearing SV40 tumors was also found to give nuclear fluorescence in susceptible cells (AGMK or BSC-1) acutely infected with SV40 virus. These findings provide further evidence that cellular incorporation of the SV40 viral genome, with partial expression of the genome by synthesis of at least one virus-specific antigen, is an integral property of all SV40 transformed cells.

Animals↗
PubMed1964

NEW PAPOVAVIRUS CONTAMINATING SHOPE PAPILLOMATA.

A virus having the characteristics of the papovaviruses was isolated from several naturally occurring cottontail papillomata. Serologic and animal inoculation studies indicate that it is not Shope papilloma virus, but a previously undescribed, nonpathogenic agent of cottontail rabbits.

Animals↗
PubMed1963

Milker's nodules: isolation of a poxvirus from a human case.

A virus of the pox group was isolated from a lesion on a patient with milker's nodules by use of tissue cultures of bovine cells. The virus shows marked biologic and serologic differences from vaccinia virus, and it is presumably the etiologic agent of the milker's nodule syndrome.

Animals↗