Biomedical subjects
Publications and source records attributed to W Płonka.
In 1999, 437 intestinal cestode cases were registered in Poland. Among them 369 were caused by Taenia saginata, 7 by T. solium, 35 by Taenia sp., 3 by Hymenolepis nana and 2 by Diphyllobothrium latum and 39 cases of echinococcosis were also registered. The obtained results confirmed the decreasing frequency of infection with intestinal cestodes in Poland.
In 1998, 603 intestinal cestode cases were registered in Poland. Among them 516 were caused by T. saginata, 5 by T. solium, 59 by Taenia sp., 2 by Hymenolepis nana and 1 by D. latum. Moreover, 31 cases of larval cestode infections were also registered. Three of them were caused by larvae of T. solium and the remaining ones by E. granulosus. The obtained results confirmed the decreasing frequency of infections with intestinal cestodes in Poland.
In 1997, 736 intestinal cestode cases were registered in Poland. Among them 634 were caused by T. saginata, 6 by T. solium, 63 by Taenia sp, 15 by Hymenolepsis sp, and 2 by D. latum and D. caninum. Moreover, 27 cases of larval cestode infections were also registered. Four of them were caused by larvae of T. solium and the remaining ones bz E. granulosus. The obtained results confirmed the decreasing frequency of infections with intestinal cestodes in Poland.
OBJECTIVE: The studies were undertaken to: (i) evaluate the epidemiological situation of infections with intestinal parasites among seven-year-old children attending first classes of the elementary schools in 25 provinces of Poland (ii) answer the question concerning an influence of the summer flood 1997 on the epidemiological situation of intestinal parasitoses in the affected regions. METHODS: The studies included 30,110 children, i.e. 10% of the population of seven-year-olds, in the provinces under study, and were based on the examination of a single faecal specimen and a cellophane swab using the following methods: wet mount preparations in 0.9% NaCl and diluted Lugol,s iodine, zinc sulphate centrifugal flotation and decantation. RESULTS AND CONCLUSIONS: The parasites most frequently encountered in the examinations included: Enterobius vermicularis (in 16.45% of the examined), Ascaris lumbricoides (2.8%), Giardia intestinalis (1.02%), Entamoeba coli (0.73%), Trichuris trichiura (0.29%). The obtained results confirmed the decreasing frequency of infections with intestinal parasites in Poland and indicated that the summer flood of 1997 had a little influence on the epidemiological situation of intestinal parasitoses in the affected regions.
It has been suggested to change the present way of serological diagnosis of echinococcosis in Poland which utilizes immunoelectrophoresis for confirmation of positive results in a screening test. Diagnostic procedure should be indicated with a sensitive test, such as indirect haemagglutination or immunoenzymatic assay, and every positive result should be verifies later using a Western-blot method in order to detect the most specific reaction with 8 kDa fraction of Echinococcus antigen. Further diferentiation of the infecting species may be accomplished with the aid of Em2 plus ELISA test which detects antibodies specific for E. multilocularis.
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46 sera and 1 cerebrospinal fluid samples from 47 patients suspected of hydatidosis were examined by Western blot method, and the results were compared with those of the indirect haemagglutination, immunoelectrophoresis and an enzyme-linked immunosorbent assay (ELISA). The examinations confirmed high sensitivity and specificity of the reactions with 8 kDa antigenic fraction in the Western blot. The above data supported the concept, that serological diagnosis of hydatidosis should be carried out using a high sensitivity screening test, such as ELISA, then the Western blot method to verify positive results.
Circulating parasitic antigen was demonstrated in the sera of rats and patients infected with Trichinella spiralis. Using a dot-immunobinding method, the antigen was detected in the sera of experimental animals from the fourth day of infection and in more than one third of the patients by the end of the third week from infection. The method proved to be simple, sensitive and specific. It should assist an early diagnosis of trichinellosis demonstrating the antigen in some cases with negative or doubtful results for specific antibodies.
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