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Biomedical subjects

W Papierz

Publications and source records attributed to W Papierz.

At least 19 recordsLinked to original sources
PubMed1995

Prevention of chronic relapsing experimental autoimmune encephalomyelitis by soluble tumor necrosis factor receptor I.

We have evaluated the effect of the type I (p-55, type beta) soluble tumor necrosis factor receptor (sTNFrI) in an animal model of multiple sclerosis. Experimental autoimmune encephalomyelitis (EAE) was induced in SJL/J mice by adoptive transfer of T lymphocytes sensitized to myelin basic protein (MBP). sTNFrI completely blocked both clinical signs of disease and pathological changes that included CNS demyelination and inflammatory cell infiltration. Effective inhibition of disease expression was obtained using several different regimens of subcutaneous (s.c.) injection. These included daily doses starting at day 0, every other day injections starting at day 0, daily doses starting on day 4, and two doses separated by 12 h on day 1 and 2. Furthermore, treatment with sTNFrI for 15 days completely protected these animals from the recurrent episodes of disease normally associated with adoptively transferred EAE. These findings suggest that TNF plays a major causative role in EAE and that the sTNFrI may prove to be a useful therapeutic approach in multiple sclerosis.

Animals↗
PubMed1995

Diagnosis of Alzheimer's disease with commercially available anti-beta peptide (beta A4) antibodies following microwave oven pretreatment.

Alzheimer's disease (AD), the most common presenile dementia is underdiagnosed in Poland, thus every attempt to make the frequency of this diagnosis approaching standards of Western countries should be recommended. Deposits of beta A4 amyloid in a form of amyloid (senile) plaques, diffuse amyloid deposits and congophilic angiopathy is central to the pathogenesis of AD. These amyloid deposits are virtually invisible in routine pathological stainings like HE but may be visualized with Bielschowsky silver impregnation, other metallic impregnations, and following Thioflavine S or Congo red stainings. We report here that amyloid deposits are as easily immunolabeled with commercially available antibodies against beta A4 (DAKO) and such a staining was greatly enhanced by microwave oven pretreatment. In all cases of AD, the diagnosis could be easily made using either 4GM or commercial DAKO anti-beta A4 antibodies following pretreatment with formic acid or processing in microwave oven. Pretreatment in microwave oven even for only one second was already sufficient to visualize beta A4-immunopositive plaques while after 5 second the intensity of staining approached that obtained after formic acid pretreatment.

Alzheimer Disease↗
PubMed1994

Expression of N-myc, c-myc and c-erbB-1 proto-oncogenes in cerebral primitive neuroectodermal tumors (PNET).

Neuroectodermal tumors demonstrate a relatively high incidence of proto-oncogenes amplification. This study attempted to determine the frequency of overexpression of three genes: N-myc, c-myc and c-erbB-1, in human PNET. Immunohistochemical studies revealed 5 to 74% neoplastic cells with positive immunoreactivity to anti-c-myc antibody in all investigated samples. In 3 cases the reactivity was particularly strong and present in more than 50% of tumor cells. Northern blot analysis revealed normal (2.3-kb in size) but significantly higher level of c-myc transcripts in these cases. By using anti-EGFR antibody 3 tumors disclosed 10 to 65% neoplastic cells with positive immunoreactivity. There was no rearrangement detected of their c-erbB-1 or N-myc genes by means of applied methods. Our results suggest that overexpression of c-myc gene is present in 10% of PNET but it is not the consequence of gene amplification. Amplification of N-myc and c-erbB-1 are rare events during PNET development.

Brain Neoplasms↗
PubMed1994

Immunohistochemical study in two cases of dysplastic gangliocytoma of cerebellum (Lhermitte-Duclos disease).

We report here immunohistochemical study of two cases of dysplastic gangliocytoma of cerebellum (Lhermitte-Duclos disease) a rare entity which is recently classified as a tumor but which hamartomatous nature is also considered. Dysplastic cells of our cases expressed neuronal markers (synaptophysin and NFP), thus their origin from neurons or neuronal precursor is clearly demonstrated. The exact progenitor cell is, however, unknown. The glial involvement was not easily judged based on our histochemical study. While a few GFAP-immunopositive astrocytes were seen in all specimens, including the recurrence of the first case, they appeared of normal size and non hypertrophic in the second case. Furthermore, they did not much increased in number in the recurrent tumor. Thus, it seems that they are not the primary target for the pathogenetic process, whatever it is, of Lhermitte-Duclos disease.

Adult↗
PubMed1994

Ultrastructure of the primitive neuroectodermal tumors (PNET).

We report a prospective series of consecutive cases of primitive neuroectodermal tumor (PNET) studied by electron microscopy. Virtually all specimens showed a differentiation along neuroblastic lines as evidenced by the presence of neurites, dense-cored vesicles, microtubules and adhesive plaque junctions. We observed also numerous intracytoplasmic cilia and autophagic vacuoles. Synaptic specializations were only rarely seen. We conclude that PNET is a tumor category which is not undifferentiated ("primitive") and clearly exhibits features of neuroblastic differentiation.

Adolescent↗
PubMed1993

Influence of bismuth on the metabolism of endogenous metals in rats.

In this study the effect of single and multiple doses of bismuth on the metabolism and excretion of endogenous metals (Cu, Zn) was investigated. Wistar rats were administered bismuth chloride subcutaneously (1 x 3 mg Bi/kg or 7 x 3 mg Bi/kg). Rats were sacrificed at different time periods after bismuth injections. The levels of Cu and Zn were determined in the liver, kidneys and brain, as well as the concentrations of metallothionein (MT) in the liver and kidneys. Cu, Zn and total protein levels in the urine of control and exposed animals were estimated. Additionally, a histopathological examination of the kidneys and brain was carried out. The increase in the renal and hepatic Cu and Zn content was paralleled by an augmentation in the level of MT in these organs following both kinds of exposure to bismuth. Some changes in the ultrastructure of the kidneys were correlated with the dose of bismuth. Zinc content in the brains of exposed rats was lower than in control animals brains, but there was no difference in copper level and no histological changes. The excretion of copper and zinc was higher in the exposed groups than in control groups. The presented results indicate that the disturbances in the metabolism of Zn or Cu in the liver, kidneys and brain and the increase in urinary output of metals may be a much more sensitive index than morphological damage and impaired renal function to the bismuth exposure.

Animals↗
PubMed1992

Cerebral and cerebellar glial tumors in the same individual.

We report histologically different gliomas occurring simultaneously in both the cerebrum and cerebellum in a 53-year-old woman. One tumor was a cerebellar astrocytoma, and the second was a temporal glioblastoma multiforme. Two months after the removal of both tumors, the third lesion, located in the basal ganglia, was found on a computed tomographic examination, but it was not verified histologically. We recommend a biopsy of one tumor when a diagnosis of multiple brain tumors is established based on a computed tomographic examination, in order to avoid the misdiagnosis of multicentric gliomas as brain metastases.

Astrocytoma↗
PubMed1992

Creutzfeldt-Jakob disease with tubulovesicular structures: an ultrastructural study.

Tubulovesicular structures (TVS) have been consistently observed in brain tissue of the transmissible spongiform virus encephalopathies such as natural and experimental scrapie, bovine spongiform encephalopathy and experimentally induced Creutzfeldt-Jakob disease (CJD). TVS were recently demonstrated in 3 cases of naturally occurring CJD. We report here the presence of TVS in another human brain with CJD, as detected in all 3 specimens by thin section electron microscopy. Their occurrence in all types of spongiform encephalopathies, irrespective of the affected host and the strain of infectious agent, emphasizes their biological significance.

Astrocytes↗
PubMed1991

Amyloid beta-protein in cerebral amyloid angiopathy, senile plaques, and preamyloidotic lesions in subcortical arteriosclerotic encephalopathy (Binswanger disease).

Cerebral vascular amyloid deposits, senile plaques and neurofibrillary tangles have been found in subcortical arteriosclerotic encephalopathy (Binswanger disease). A mouse antiserum, prepared against a 43-amino acid synthetic peptide homologous to the amyloid beta-protein of Alzheimer disease (anti-SP43), revealed immunoreactive amyloid deposits in meningeal and intracortical blood vessels, senile plaques, intraneuronal amyloid and preamyloid in a neuropathologically confirmed case of Binswanger disease previously reported to have cerebral vascular amyloid deposits. These lesions contained sulfated glycosaminoglycans as determined by the Alcian blue/critical electrolyte concentration method. Similar findings were not observed in a case of Binswanger encephalopathy without cerebral amyloid deposits. Our study indicates that amyloidotic lesions in Binswanger encephalopathy with cerebral amyloid deposits contain amyloid beta-protein and sulfated glycosaminoglycans.

Amyloid beta-Peptides↗
PubMed1991

Demonstration of amyloid beta-protein in a 32-year-old man with progressive dementia.

We report the immunolocalization of extensive amyloid beta-protein in senile plaques, cerebrovascular amyloid deposits, neurofibrillary tangles and preamyloid in a 32-year-old man with progressive dementia not due to trisomy 21 or trauma. These amyloid deposits were non-reactive to antibodies directed against scrapie amyloid. Our data indicate that the presence of amyloid beta-protein is not limited to normal aging, Alzheimer's disease and related disorders but is also found in younger individuals with progressive dementia.

Adult↗
PubMed1991

Cranioplasty of an extremely large cranial defect caused by transitional meningioma with a knitted polypropylen-polyester prothesis "Codubix".

The authors report a case of transitional cell meningioma of the convexity which destroyed a large portion of the calvarium and invaded subcutaneous tissue. The tumour was totally removed and a large cranial defect/430 cm2 in size/was filled with a polypropylenopolyester knitted prothesis "Codubix" with an excellent result. The problems of chronioplastic closure of such an unusually large skull defect and the advantages of the use of the material "Codubix" are discussed.

Adult↗
PubMed1988

Cerebral amyloid angiopathy with attenuation of the white matter on CT scans: subcortical arteriosclerotic encephalopathy (Binswanger) in a normotensive patient.

Subcortical arteriosclerotic encephalopathy was diagnosed in a 56-year-old female normotensive patient with gradually progressing dementia, pseudobulbar palsy and motor deficits. CT scan showed white matter low attenuation in the frontal and parietal lobes. Neuropathological examination revealed degeneration of the white matter. Amyloid was found in walls of small cortical vessels. The walls of small vessels in the white matter showed severe thickening, fibrosis and hyalinization but not amyloid. Cerebral amyloid angiopathy may be responsible for subcortical arteriosclerotic encephalopathy.

Amyloid↗
PubMed1988

A case of multicentric glioma of cerebellum and brain.

A case of true multicentric glioma which occurred in a 52-year-old female is presented. Two lesions one occurring six months after the other were localized below and above the tentorium, respectively. Histological examination showed the same pattern of anaplastic astrocytoma in both foci. The possibility of cerebellar tumor metastasizing to cerebral hemisphere is discussed and deemed as unlike in this particular case.

Astrocytoma↗
PubMed1987

Creutzfeldt-Jakob disease with plaques and paired helical filaments.

A 32-year-old man with rapidly progressive dementia, pyramidal signs, myoclonic jerks and dystonic movements died following brain biopsy. neuropathological examination revealed minimal neuronal loss accompanied by mild spongiform change and astrocytic reaction. Numerous plaques and neurofibrillary tangles composed of paired helical filaments dominated the ultrastructural picture. This patient had features of both Creutzfeldt-Jakob disease and Alzheimer's disease, providing additional support for the existence of an overlap between these disorders.

Adult↗
PubMed1987

Clinical course and surgical results in supratentorial low-grade gliomas.

From 1968-1985 505 supratentorial primary brain tumors were operated on in Department of Neurosurgery of Medical Academy of Lódź, 49 of them (9%) were low-grade gliomas. Basing on WHO classification we found 29 fibrillary, 14 protoplasmatic, 1 pilocytic astrocytomas and 5 oligodendrogliomas. There were 19 men and 30 women. 70% of patients were 21-50 years old, and there were 5 children aged from 4 to 15 years. The initial symptoms were headache and epilepsy which occurred in 70% of astrocytomas and in all 5 cases of oligodendrogliomas. Only 2 patients presented symptoms of intracranial hypertension. The history of illness was shorter than 1 month in 10 patients (20%) and longer than 2 years in 20 (40%). 29 tumors were lobar with basal ganglia infiltration, 4 were located in central part of the brain and 16 were strictly lobar. These 16 tumors were removed totally and from 14 patients who survived operation 3 died of recurrence 3-5 years after surgery, but all the others are still alive, 7 of them longer than 5 years. In 19 cases tumor was removed partially, 4 patients died in postoperative period, 8 of recurrence 8 months to 4 years after surgery, but 7 is still alive, 4 longer than 5 years. In 14 cases only biopsy was performed, 7 patients died in first 12 months, 2 on the second and 1 one the fourth year after biopsy. 4 patients is alive longer than 1 year.

Adult↗