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PubMed1995

[Clinical studies on changes in sexual hormones and estrogen receptor in patients with gynecomastia].

Levels of serum testosterone (T), estradiol (E2), and estrogen receptor (ER) in the tissue were detected by radioimmunoassay and immunohistochemical method (ABC technique) in 46 patients with gynecomastia. The results showed that rise of E2 and E2/T is a whole-body etiologic factor of hypertrophy of male breast, and the positive rate of ER is an important local etiologic factor of gynecomastia. E2 and ER have aosynergism effect on onset and development of gynecomastia and cause the ductal cell hyperplasia of male breast. It should be alerted whether it can cause male breast cancer.

Adult↗

PubMed1991

Expression of firefly luciferase gene in Xenopus laevis oocyte.

Fusion plasmid pSV-Luc20 and pSV-Luc19 were constructed by using the larger BamHI restricted fragments of plasmid pDO432 containing the firefly luciferase (luc) gene and HindIII-BamHI restricted fragment of plasmid pSVK100 containing the SV-40 promoter as insert and vector, respectively. The fusion plasmid had different ligation orientations between the encoding sequence of luc gene and the SV-40 promoter, i.e., they were sense and antisense plasmid. When they were introduced into Xenopus oocyte by micro-injection, only pSV-Luc20 could be transcribed and translated into the enzymatic protein of luciferase with high biological activity. Therefore, firefly luciferase gene and Xenopus oocyte can be used as an excellent system for monitoring the activity of various promoters.

Animals↗