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W Pfeiffer

Publications and source records attributed to W Pfeiffer.

36 records · Page 2Linked to original sources
PubMed1976

Specific cleavage of chromatin by restriction nucleases.

Digestion of mouse and rat liver nuclei with a restriction nuclease from Bacillus subtilis (Bsu) is examined in continuation of previous work from this laboratory (Pfeiffer et al., 1975, Nature 258, 450). The finding of more than 95% C in the 5'-termini of the DNA fragments generated during digestion with Bsu shows that the participation of endogenous nucleases in Bsu digestion is extremely small. The restriction nuclease Hae III, an isoschizomer of Bsu, yields identical degradation patterns. The patterns conform to what one expects from statistical calculations based on a nucleosome structure of chromatin with a region preferentially accessible to the nuclease of 40-50 nucleotide pairs per nucleosome. Integrity of the histones is maintained during digestion with restriction nucleases. Digestion of mouse liver nuclei with EcoRII shows that most if not all of the satellite DNA is organized in a nucleosome structure. Also in rat liver, much of the repetitive DNA appears to be present in nucleosomes.

Animals↗
PubMedDate not supplied

Sexual disorders in nonacute psychiatric outpatients.

One hundred schizophrenics and 58 patients with an affective psychosis who were in relatively stable condition and were being treated as outpatients, as well as 30 patients receiving dermatological treatment, were questioned in a semistructured interview about their sexual history. Forty-nine percent of schizophrenics, 36.2% of those with affective disorders, and 13.3% of control patients had a sexual dysfunction. The most frequent form experienced by psychiatric patients was hypoactive sexual desire disorder. Underlying causes were presumed to be multifactorial in most cases (expert rating). The patients most frequently affected by sexual dysfunctions were schizophrenics on neuroleptic medication, whereas schizophrenic patients not on any medication had fewer dysfunctions and control patients were only minimally affected. Neither the nature of the psychopharmaceuticals nor the dose level had a specific influence on the frequency of sexual dysfunctions.

Adult↗
PubMedDate not supplied

[What makes a consultation therapeutic? On the cultural characteristics of psychotherapeutic methods].

Transcultural comparisons relativize the universal validity of the modern methods of psychotherapy and highlight their close relationship to "world view" and style of living. Although traditional healing rites do have a lot in common with our methods oriented towards "insight", "empathy" and "understanding" (such as motivation, creation of an emotionally significant relationship, interpretation of the disease and its treatment according to a plausible concept), certain aspects are specific for our way of proceeding (such as individuation and reason), whereas other characteristics on which we focus less attention occupy a central position in traditional healing rites (e.g. social integration, involvement of the body, actions). Such "gaps" suggest that it should be of advantage to "transfer" some of the therapeutic techniques from one cultural environment to another (e.g. from an alien culture to our way of life or vice versal). That, however, is possible to a very limited extent only and demands critical and modifying integration, since therapeutic methods are very closely connected with the central values of the relevant culture in respect of their particular form of relationship to that culture and the aims of that culture.

Cross-Cultural Comparison↗