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Biomedical subjects

W Poppe

Publications and source records attributed to W Poppe.

At least 19 recordsLinked to original sources
PubMed1992

[Diagnosis of chronic pancreatitis. Studies of duodenal juice after stimulation with the secretin-ceruletide test. Decision limits and evaluation of various parameters].

187 patients were checked up over 4 years by the secretin-ceruletide test. Independently of the test results they were assigned to various disease groups on the basis of clinical assessment. 131 subjects were divided in a pilot investigation into: subjects with a healthy pancreas (n = 55); subjects with chronic pancreatitis (n = 50); subjects whose pancreatic condition could not be classified clearly (n = 26). 8 parameters were compared by univariate and multivariate statistical procedures in order to confirm or rule out the presence of chronic pancreatitis. The discriminatory power of the following parameters in duodenal fluid proved to be sufficiently high, with less than 15% frequency of misclassification: chymotrypsin (activity) and/or; lipase (activity) and/or; amylase (activity); viscosity. Under routine conditions measurement of the activity of two of these enzymes is sufficient. Their contribution to discrimination proved to be approximately equal. The diagnostic sensitivity and specificity of the parameters bicarbonate, lipase (concentration), trypsin (activity) and volume of duodenal fluid are lower. The classification rules derived from the above pilot group were confirmed by a diagnostic study under routine condition in a test group of 38 patients. Limitation to examining only volume and a maximum of 3 parameters which proved best in distinguishing between patients with chronic pancreatitis and healthy subjects, together with the omission of the first-hour samples after a secretin bolus, considerably reduced laboratory workload without altering the discriminatory power of the secretin-ceruletide test.

Adult↗
PubMed1989

Evaluation of a new assay for pancreatic amylase: performance characteristics and estimation of reference intervals.

We have evaluated a new assay for the specific determination of pancreatic (P) isoamylase, the principle being based on a synergistic inhibitory effect of two monoclonal antibodies directed towards salivary (S) amylase. After 3 min incubation, activities were determined with maltoheptaoside-PNP as substrate on a Hitachi 705 analyzer at 25, 30 and 37 degrees C, respectively. Coefficients of variation ranged from 0.6 to 5.4% for within-run and 2.1 to 9.9% for day-to-day precision. Linearity held up to 1200 U/L (25 degrees C) and 2300 U/L (37 degrees C). Comparison of the new method with the wheat germ inhibitor technique showed an excellent correlation, with coefficients ranging from 0.990 to 1.00. Using purified P- and S-amylase we observed no inhibiting cross-reactivity of the antibodies with the P-isoenzyme, but an incomplete blockage of S-amylase: residual activity was approximately 2% at 25 and 30 degrees C, and 2.5% at 37 degrees C. The distribution pattern of P-, S- and total amylase activity in serum of healthy subjects was only slightly skewed to the right. We found neither an influence of sex nor of age on the reference ranges. In random urine samples, distribution of activities was strongly skewed. However, if the activity was related to the urinary creatinine concentration, an approximately normal distribution was obtained, allowing, as in serum, the establishment of upper and lower reference values.

Adolescent↗
PubMed1989

[Problems of electroconvulsive treatment in advanced age--its possible applications and necessities, indications criteria, method and effectiveness].

After a critical discussion with antiquated and actual problems to the electroconvulsive therapy the problem-important peculiarities of progress and treatment of the psychosis in the higher age are exhibited. In search of satisfactoring solutions we used Dexamethazon as a supplemental premedication in the electroconvulsive therapy and by this way we found out a remarkable minimize of complications which allowed to put in more frequently the electroconvulsive therapy during the psychosis in the higher age. This leads to a general optimation of prognosis of the late psychical diseases. Beside the method itself the urgency of indications and exclusion of standards are described in the same way, also there are shown the few complications in comparison with the successes of therapy of 2010 electricalconvulsive therapies from 433 patients.

Aged↗
PubMed1989

Evaluation of a new alpha-amylase assay using 4.6-ethylidene-(G7)-1-4-nitrophenyl-(G1)-alpha-D-maltoheptaoside as substrate.

The determination of alpha-amylase activity using an ethylidene-blocked 4-nitrophenyl-maltoheptaoside (EPS) has been evaluated in five laboratories on eight different analysers at 25 degrees C, 30 degrees C and 37 degrees C. The protecting ethylidene group inhibits hydrolysis at the non-reducing end of the substrate molecule by the auxiliary enzyme, alpha-glucosidase. The combined reagent is therefore stable for at least 10 days at 2-8 degrees C. HEPES is used, because the molar absorbance of 4-nitrophenol is independent of temperature in the presence of this buffer. Compared with the method using unprotected substrate 4-nitrophenyl-alpha-D-maltoheptaoside (4NP-G7), the present method is equal or better with respect to the imprecision, linearity and interlaboratory transferability of results in human and control sera. Since the protected and unprotected substrates differ in their turnover rate, the new assay yields activities which differ from those of the 4-nitrophenyl-alpha-D-maltoheptaoside method. Based on the homogeneous results obtained in method comparisons between EPS and 4-nitrophenyl-alpha-D-maltoheptaoside, and in order to maintain the 4-nitrophenyl-alpha-D-maltoheptaoside reference values, a conversion factor was derived to eliminate the above differences: activityEPS x 2.50 = activity4NP-G7. The temperature and instrument independence of this relationship was demonstrated in a total of 720 human sera and plasmas.

Glucosides↗
PubMed1987

Pregnancy after transcatheter embolization of a uterine arteriovenous malformation.

A 25-year-old woman with a congenital uterine arteriovenous malformation had a long history of repeated excessive vaginal bleeding. She was successfully treated with transarterial embolization. She had normal menstrual periods for 6 months and subsequently conceived. She was delivered of a normally grown baby at 35 weeks. To the best of our knowledge, this is the third pregnancy described after successful embolization of an arteriovenous malformation.

Adult↗
PubMed1987

A multicentre evaluation of the Boehringer Mannheim/Hitachi 704 analysis system.

The selective multitest Boehringer Mannheim/Hitachi 704 analysis system was examined according to the ECCLS guidelines in a multicentre evaluation involving four laboratories. Ten routine parameters, covering most of the application settings of the instrument, were measured in the respective laboratory at temperatures 25, 30 or 37 degrees C. The trial lasted four months and gave more than 40,000 data. It yielded the following results: 1. Within the four laboratories the mean coefficients of variation for three control sera at different concentrations were found to be equal to or better than 1.6% for the within-run imprecision and 2.8% or better for the between-day imprecision. 2. No drift was observed during eight hours. 3. Because of the high linear measuring range a re-run analysis was seldom necessary. 4. Sample-related carry-over was not seen. Reagent-dependent carry-over was measured from cholesterol to uric acid and from triacylglycerols to lipase. Through modification of the cholesterol and triacylglycerol reagents, the carry-over effect was practically eliminated. 5. The recovery of the assigned values of control sera showed average values between 99 and 104%. For bilirubin, creatinine, creatine kinase and alanine aminotransferase some control sera showed deviations greater than 10%. 6. In all cases, regression analysis of the results obtained in comparisons of the present instrument with the Hitachi 705 or 737 yielded slopes close to unity with extreme values of 0.95 and 1.06. 7. During the entire evaluation period there was no malfunction or breakdown of the instruments. The evaluators came to the conclusion that the analytical performance as well as the reliability and practicability of the Hitachi 704 can be rated as excellent.

Autoanalysis↗
PubMed1986

Transcatheter embolization of uterine arteriovenous malformations.

A case of congenital arteriovenous malformation of the uterus in a 25-year-old woman with a long-standing history of recurrent menometrorrhagia is reported. Radiological diagnosis was based on hysterographic, computed tomographic, and angiographic findings. Treatment was performed with percutaneous transcatheter embolization.

Adult↗
PubMed1986

Evaluation of a new inhibitor test for isoamylase on Hitachi 705 analyser.

We describe a simple and rapid method for measuring pancreatic and salivary type amylases using the Hitachi 705 analyser. The determination is based on the inhibition of salivary amylase using an inhibitor isolated from wheat germ. The precision of the proposed method was very good: within-day precision varied from 0.4 to 2.5% (CV) and day-to-day precision from 2.2 to 3.7% (CV). The new application correlated well with another commercially available inhibition method. As the standardization is very stable and the assay procedure exactly the same as for the total amylase assay, the proposed method is suitable for routine isoamylase determination. Reference values for pancreatic and salivary amylase activities are presented.

Autoanalysis↗
PubMed1985

Five methods for measuring low-density lipoprotein cholesterol concentration in serum compared.

Five methods for the quantification of low-density-lipoprotein cholesterol were compared: ultracentrifugation, electrophoresis, precipitation with polyvinyl sulfate or heparin, and an indirect calculation procedure (Friedewald formula). Excellent agreement of results was obtained with all procedures for 49 of 51 sera. Discrepancies were as much as 1.69 g/L for the remaining two cases, which contained appreciable amounts of "floating" beta-cholesterol as detected with a combination of ultracentrifugation and electrophoresis.

Chemical Precipitation↗
PubMed1985

[Probability in the clinical diagnoses of Pick disease and Alzheimer disease].

This paper is dedicated to the honour of Prof. Dr. Erich Sternberg. The diagnostical strategy of well-known specialists for the Morbus Pick and Morbus Alzheimer is considered. As a result of our own experiences and analyses the psychopathological factors are assigned to the following groups: change of vitality; change of personality; reversible organic psychoses; fuzzy brain-local symptoms; development of dementia. For these groups growth curves are given for which the analytic description by a special type of a differential equation, is used. The different behaviour of the development of the dementia to the Morbus Pick and Morbus Alzheimer is found.

Alzheimer Disease↗
PubMed1983

Investigations on the mechanism of photodynamic action of different psoralens with DNA.

Investigations on the photodynamic action of psoralens with DNA were performed, using experimental techniques of fluorescence lifetime and NMR-CIDNP, as well as SCF-MO and CNDO molecular orbital calculations. It has been shown that the formation of a biradical through the triplet state is the decisive step for psoralen dimer formation, as well as for cyclobutane addition with thymine, while singlet oxygen production is responsible for enzyme inactivation (e.g., lysozyme and trypsin). The molecular orbital calculations, in agreement with experimental results, indicate that the differences in biological effectivity of different psoralens are based on variations in triplet formation probability.

Animals↗
PubMed1981

[Alcohol and central and peripheral nervous system disorders].

It belongs to the definition of chronic alcoholism that also clinically established lesions of the organs can be proved. As to the nervous system the case in question are multilocular defects which concern the central and peripheral nervous system. The local accentuation, the developmental speed and the intensiveness of the alcoholic defect of the cellular metabolism lead to different courses. The suggestion of their individuality must at the same time take into consideration the character of chronic alcoholism which on principle affects the whole nervous system.

Alcohol Amnestic Disorder↗
PubMed1979

[Vegetative phenomena in the course of depressive states].

Depressive psychoses are accompanied by vegetative disorders. Third-order blood-pressure waves are an expression of vasomotor rhythms which through diencephalic and limbic structures tend to adjust the blood pressure to the respective overall psychovegetative situation. In the case of depressions with an axious increase of impulse, statistical evidence was obtained, within the framework of clinical improvement, for a correlation between a decreasing score of depression and an increasing frequency of third-order waves. The same central trends of these quantities suggest that both of them are different manifestations of a common functional disorder in the limbic system and diencephalon.

Affective Disorders, Psychotic↗
PubMed1976

[Psychiatric and neurological problems of the older age (author's transl)].

Pyschical crisis and pathological change do not differ at people in the older age. The two terms do not characterize the normal development of the old age, but extreme spheres are circumscribed. The cerebrovascular insufficience is a difficult diagnostic and therapeutic area, but it is accissible. The psychopathological symptoms to be caused by this disturbance are reversible in many cases. The problems of a comprehensive diagnostic and therapy of sleeping-disturbances at aged people are described.

Aging↗