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W R ADAMS

Publications and source records attributed to W R ADAMS.

At least 19 recordsLinked to original sources
PubMed1963

Epizootic diarrhea of infant mice: indentification of the etiologic agent.

Electron micrographs of intestinal epithelium of infant mice infected with epizootic diarrhea virus have demonstrated intracellular spherical structures measuring 65 to 75 m(micro) in diameter which have a complex morphology resembling several virus particles. They have ben interpreted as being the etiologic agent of this disease. The particles were present in association with, in some cases within, vesicles of the endoplasmic reticulum of intestinal epithelial cells. They were never seen in the nucleus.

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PubMed1957

An electron microscopic study of incomplete virus formation; infection of Ehrlich ascites tumor cells with chick embryo-adapted Newcastle disease virus (NDV).

The morphologic changes occurring in Ehrlich ascites tumor cells infected with chick embryo-adapted Newcastle disease virus were studied with phase microscope, conventional light microscope, and electron microscope. Intracytoplasmic inclusions appeared 2 to 4 hours following infection and progressively increased in size and numbers until cytolysis occurred. No significant alterations in mitochondria or other cell organelles were detected during the initial period of inclusion development. The inclusions were composed of a multilaminated shell, probably derived from the agranular reticulum of the EAT cell, surrounding an inner core packed with dense particles, measuring 3 to 14 mmicro in diameter. These particles were tentatively identified as the newly synthesized "incomplete virus" which had been previously demonstrated by fluorescent antibody techniques. The possible role of the inclusions is discussed.

Animals↗
PubMed1957

An electron microscope study of the morphology and distribution of the intracytoplasmic virus-like particles of Ehrlich ascites tumor cells.

Electron microscope studies of eight different sublines of Ehrlich ascites tumor cells which had not, as far as could be determined, come in contact with any known virus, revealed dense particles measuring approximately 55 to 70 mmicro in diameter, both within and attached to the wall of cytoplasmic vesicles identified as the endoplasmic reticulum. All tumor sublines contained significant numbers of particles and revealed no qualitative or quantitative differences in particle morphology or distribution. It is concluded that these structures are a constant morphological component of the Ehrlich ascites tumor and that they probably do not represent contaminating virus. Their morphology and distribution are described, and the possible interpretations of their significance are discussed.

Animals↗