Gene transfer and amplification in CHO cells. Efficient methods for maximizing specific productivity and assessment of genetic consequences.
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Animals↗
Biomedical subjects
W R Arathoon
Publications and source records attributed to W R Arathoon.
Large-scale cell culture in biotechnology.
The purpose of this article is to review the current state of large-scale cell culture in terms of its applications, problems, and potential. Because of the commercial and proprietary nature of most large-scale cell culture processes, this review does not contain many detailed scientific results although an attempt is made to address some key issues and findings. Much of this summary deals with processes having an established, commercial track record but some attention is given to more recent innovations with interesting potential applications. Reference is made to plant cell culture but the main emphasis is on mammalian cells.
Animals↗
Uptake of amino acids and glucose by BHK 21 clone 13 suspension cells during cell growth.
The uptake of amino acids and glucose by BHK 21 clone 13 suspension cells grown in Modified Eagles Medium was monitored. The Growth Yields for sixteen amino acids and glucose were determined. Four "non essential" amino acids were utilised. Glutamine, glutamate, leucine and asparagine were used in greatest quantity by the cells which also utilised virtually all the cystine in the medium. The implications of these results are discussed.
Amino Acids↗