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Biomedical subjects

W R Boone

Publications and source records attributed to W R Boone.

11 recordsLinked to original sources
PubMed1991

The female reproductive cycle: new variations on an old theme.

Understanding the female reproductive cycle is the essence of the practice of reproductive medicine. This article reviews important publications that shine new light on the female reproductive cycle. Menstrual dysfunction can begin at adolescence and persist throughout the reproductive years. Adolescent women with menstrual dysfunction should be evaluated and treated. Approximately 30% of pregnancies terminate in abortion during the first trimester. New epidemiologic data on the etiology of spontaneous abortion are reviewed. Advancing age is associated with a decline in fecundity. Delay in childbearing and advances in assisted reproductive technologies have enabled reproductive endocrinologists to better understand the effects of advancing age on reproductive function. The most important paper that we received supports the timing of breast cancer to the luteal phase of the menstrual cycle. There was a statistically significant increase in 10-year survival in women who had surgery during the luteal phase compared with the follicular phase.

Abortion, Spontaneous↗
PubMed1990

Relative efficiency of therapeutic donor insemination using a luteinizing hormone monitor.

A prospective randomized study was performed to evaluate the use of a urinary luteinizing hormone (LH) detection kit with 1 insemination as compared with 2 alternate day inseminations with timing based on previous cycle length and basal body temperature changes. The study involved 60 patients who underwent a total of 264 therapeutic donor insemination cycles using cryopreserved semen specimens. Patients alternated LH-kit timed cycles with cycles timed by non-LH methods for a total of 6 cycles or until pregnancy was achieved. Fecundability rates were 12.3% for LH-kit cycles and 5.3% for non-LH method cycles. The difference in outcome was not statistically significant. However, when the LH kit plus 1 insemination was compared with 2 inseminations timed by conventional methods, there appeared to be a distinct monetary and time expenditure advantage. These findings suggest that sufficient advantage may be derived from use of an LH kit to recommend its use on a routine basis for the timing of therapeutic donor insemination.

Adult↗
PubMed1989

Correlation of human spermatozoa heparin binding with the zona-free hamster egg in vitro penetration assay.

The purpose of our research was to determine whether heparin-binding characteristics of human spermatozoa are related to fertilizing potential, as determined by the hamster egg in vitro penetration assay. Penetration rates were standardized (hamster egg in vitro penetration assay index) by comparison with semen from fertile controls in each bioassay. Saturation of heparin-binding domains was achieved in 100% of raw ejaculates (prewash), but in only 53% of "swim-up" (postwash) samples. The dissociation constants ranged from 0.31 to 48.75 nmol/10(6) cells, and binding site concentrations from 0.47 to 20.82 x 10(17) binding sites/cell. Heparin-binding affinity was significantly greater in prewash compared with postwash samples (p less than 0.01). In prewash samples the number of binding sites differed significantly between subjects having low and high penetration indices (5.67 +/- 1.05 vs 2.01 +/- 0.34 x 10(17) binding sites/cell, p less than 0.05). In prewash samples, binding affinity for heparin significantly correlated with hamster egg in vitro penetration assay indices (R2 = 0.142, p less than 0.05). In contrast, the number of binding sites in prewash samples was negatively correlated with hamster egg in vitro penetration assay indeces (R2 = 0.201, p less than 0.05). These data indicate that the heparin-binding assay may prove to be a rapid, sensitive, and inexpensive means of assessing fertilizing potential of human spermatozoa.

Animals↗
PubMed1989

Computer-aided pattern analysis of temperature differentials.

Chiropractic analysis often incorporates the evaluation of heat distribution patterns observed in skin temperature profiles. Historically, temperature recordings of the spine have been made with a heat detection instrument using a thermocouple design. The advent of refinements in infrared technology, however, have greatly enhanced temperature detection in terms of accuracy and reliability. The present research reflects the development of computer software designed to express the data obtained with a dual channel heat sensing instrument. Two phases are involved: a) digital information, received from the sensing instrument, is standardized, stored for future analysis, and retrieved for comparisons with other graphs; b) stored data is graphically displayed, statistically analyzed, and otherwise compared. The data is displayed for visual observation as a) temperature data received from either channel of the sensing instrument or b) relative temperature differences between data from both channels of the sending instrument. For statistical evaluation, corresponding readings (comparisons between graphs) are plotted against one another and analyzed by a moving Pearson Product Moment correlation and moving t-test. This evaluation is graphically represented with a numeric display of pertinent statistical values. Current work indicates that a 10 point moving correlation and t-test will yield accurate comparisons between graphs.

Algorithms↗
PubMed1988

Freezing of mammalian embryos without the aid of a programmable freezer.

A simple, inexpensive and repeatable method of freezing/thawing (F/T) mammalian concepti was developed with the use of 2-cell mouse embryos. Cryoprotectants, length of exposure to protectants, and subzero holding times before liquid nitrogen (LN2) exposure were examined in an effort to obtain an effective freezing protocol. Sequential examination of these variables provided data suggesting that 3.5 M dimethyl sulfoxide (DMSO) plus 0.25 M sucrose exposure for 5 minutes at room temperature, followed by a -30 degrees C environment for 90 minutes, best prepared embryos for LN2 storage. After thawing and culture, 48 of the 93 (52%) embryos developed to the blastocyst stage.

Animals↗
PubMed1988

Improved cryopreserved semen fecundability in an alternating fresh-frozen artificial insemination program.

This study with glycerol and increased number of motile sperm inseminated showed an increase in f to 10.4% for frozen semen and 27.4% for fresh semen. There is a significant (P less than 0.01) difference in pregnancy rate when greater than 100 million motile sperm are inseminated (31.3%) than when less than that number are inseminated (11.9%) in the first cycle when frozen semen is used.

Female↗
PubMed1988

Relationship of heparin binding with computer-analyzed physical traits of human sperm.

The objective of this study was to determine whether heparin affinity or the concentration of binding sites was related to sperm traits analyzed by a routine computerized semen analysis in human males requesting infertility evaluations. Saturation of heparin binding sites on sperm was achieved in 36 of 50 samples analyzed so that accurate estimates of dissociation constants (Kd) and binding site concentrations could be made. A broad range in Kds (18.2 to 284.5 nM/micrograms deoxyribonucleic acid [DNA]) and binding site concentrations (2.70 to 44.77 nM/micrograms DNA) was found. The binding affinity of sperm for heparin was significantly correlated with concentrations of cells in the ejaculate (r = 0.56), concentrations of motile sperm (r = 0.51), percentage of motile sperm (r = 0.33), and total numbers of ejaculated sperm (r = 0.37). The concentration of heparin binding sites also was correlated with concentration of cells in the ejaculate (r = 0.60), concentration of motile sperm (r = 0.50), percentage of motile sperm (r = 0.40), and total number of ejaculated sperm (r = 0.49).

Binding Sites↗
PubMed1988

Effects of a chiropractic adjustment on changes in pupillary diameter: a model for evaluating somatovisceral response.

The relationship between a cervical chiropractic adjustment, in subluxated vs. unsubluxated subjects, and autonomic response monitored as change in pupillary diameter was evaluated in 15 subjects. The results indicate that: a) a successful adjustment elicits either a parasympathetic or sympathetic response; b) the vertebral level at which the adjustment is administered has undetectable specificity for the parasympathetic or sympathetic input to the pupil; c) unsubluxated subjects generally exhibit no change in pupillary diameter following a sham adjustment and d) subluxated subjects exhibit variable preadjustment pupillary diameters, with significant pupillary diameter changes in response to an adjustment. These data suggest that autonomic input to the pupil may be influenced by subluxation, as well as the magnitude and direction of force exerted during the chiropractic adjustment. An anatomical pathway through which the observed responses may occur is proposed.

Adult↗