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Biomedical subjects

W R Dito

Publications and source records attributed to W R Dito.

5 recordsLinked to original sources
PubMed1978

Automated rheumatoid factor assay in a population of patients with rheumatic disease.

Rheumatoid factor has been measured in 128 patients having rheumatic disease to compare the Singer-Plotz assay with an automated particle size assay. Correlation coefficient between the two methods using regression analysis revealed r = 0.89, P less than 0.00001 when two different commercial sources of sensitized latex particles were used. Thus rheumatoid factor may be quantitated electronically and represented in arbitrary units (MPRF) without altering clinical interpretation. This methodology may be applicable to many other immunologic assays.

Autoanalysis↗
PubMed1976

Microdetermination of serum calcium by parallel fast analyzer.

An adaptation of a direct microdetermination of serum calcium based upon its colored reaction product with cresolphthalein complexone for use with a parallel fast analyzer is described. It is simple, reliable and clinically accurate. Twenty microliters of sample are employed in a final volume of 650 mul. Comparison studies with an atomic absorption method resulted in a correlation coefficinet (r) of 0.94. Replicate analyses at normal (8.5 mg. per dl.) and abnormal (12.1 mg. per dl) levels showed inter-day coefficients of variation of 3.2 and 2.3%, respectively. Assays of sera of male and female donors (age range: 17--60 years) showed normal ranges of 8.5--10.8 and 8.5--10.5 mg. per dl., respectively. Moderate hemolysis and jaundice and mild lipemia are associated with spuriously high results. Magnesium in serum showed no significant effect below 4.0 mEq. per l.

Adolescent↗