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Biomedical subjects

W Regelson

Publications and source records attributed to W Regelson.

At least 37 records · Page 2Linked to original sources
PubMed1988

Physicochemical characterization of [3H] DHEA binding in rat liver.

Dehydroepiandrosterone (DHEA), the native clinical steroid and steroid precursor, may have a targeted physiologic role. A high affinity (Kd 2.3nM) and steroid specific [3H] DHEA binding macromolecule in male Sprague-Dawley rat hepatic cytosol suggests that DHEA may have receptor mediated physiologic action. 3[H] DHEA binding was highest in the liver followed by kidney and testis cytosols. Sulfhydryl reagents such as N-ethylmalemide and iodoacetamide inhibited the binding of [3H]DHEA by up to 60-70%. The DHEA-macromolecular complex was stable at 35 degrees C. and addition of 5mM molybdate or 0.3M KCl increased stability. Interestingly, rat liver cytosol, specific binding at 4 degrees C increased by almost 40-50% with addition of 0.1M NaSCN or 0.3M KCl. Sucrose gradient analyses showed a 7-8 S macromolecular complex in the low salt and 3-4 S complex under high salt conditions. The [3H] DHEA- macromolecular complex shows minimal temperature dependent activation in vitro at 25 degrees C as judged by binding to DNA-cellulose. The results suggest a specific high affinity macromolecule for DHEA in rat liver cytosol with unique physicochemical properties.

Animals↗
PubMed1988

Protection against acute lethal viral infections with the native steroid dehydroepiandrosterone (DHEA).

A significant protective effect of a native adrenal steroid, dehydroepiandrosterone (DHEA), was demonstrated in studies of two lethal viral infection models in mice: systemic coxsackievirus B4 and herpes simplex type 2 encephalitis. The steroid was active either by long-term feeding or by a single subcutaneous injection. A closely related steroid, etiocholanolone, was not protective in these models. Histopathological analysis, leukocyte counts, and numbers of spleen antibody forming cells in the coxsackievirus B4 model suggests that DHEA functions by maintaining or potentiating the immune competence of mice otherwise depressed by viral infection. DHEA was not effective in genetically immunodeficient HRS/J hr/hr mice and did not demonstrate antiviral activity in vitro. While the molecular basis for DHEA's effect on the immune system is not known, studies by others suggest that it may counteract the stress related immunosuppressive effects of glucocorticoids stimulated by viral infection. Because DHEA is a native steroid that has been used clinically with minimal side effects, the utility of DHEA in the therapeutic modulation of acute and chronic viral infections including the acquired immune deficiency syndrome deserves intensive study.

Animals↗
PubMed1980

Phase I study of ethylbis(2,2-dimethyl-1-aziridinyl) phosphinate (AB-163).

Ethylbis(2,2-dimethyl-1-aziridinyl)phosphinate (AB-163) was used to treat 27 patients in a phase I trial. The limiting toxicity on a weekly schedule of IV administration involved nausea and vomiting associated with a variety of cholinergic side effects, including possible seizures. A starting dose of 300--400 mg/M2/week is suggested for a Phase II trial. One partial response in a patient with squamous-cell carcinoma of the cervix metastatic to the lungs was seen.

Adult↗
PubMed1980

Keratopathy after oral administration of tilorone hydrochloride.

Two patients given tilorone HCl orally for varying periods of time had clinical and histopathologic ocular changes. Retrospective study of 14 cancer patients who were taking tilorone HCl orally revealed that three patients had similar ophthalmic findings accompanied by the appearance of blue halos around pinpoint light sources. Examination revealed a diffuse clouding of the epithelium sometimes associated with subepithelial infiltrates. Abnormalities seen histologically included cloudy swelling of the epithelium and cytoplasmic inclusions. By electron microscopy these were found to be myelinoid bodies. Gas chromatography and mass spectrophotometry showed that tilorone HCl was present in the cornea and conjuctiva. Visual acuity was not affected and these changes were slowly reversible with the cessation of therapy. Biomicroscopic and conjunctival cytologic examination may serve to indicate the drug's storage and potential damage in the body.

Adult↗
PubMed1980

Effect of pulsed high frequency electromagnetic radiation on embryonic mouse palate in vitro.

Palatal shelves from 14-day-old embryonic mice were exposed to a 27.12 MHz pulsed non-ionizing radiofrequency (Diapulse) for 20 min followed by 24-hour organ culture in nutrient agar. Diapulse-treated palatal shelves showed induction of cartilage within the mesenchymal compartment and loss of the overlying epithelium in contrast to controls which were free of cartilage. The results are thought to be independent of thermal changes and may be due to calcium flux within the tissue.

Animals↗
PubMed1979

Prospective comparison of intralesional and multipuncture BCG in recurrent intradermal melanoma.

Fifty-nine patients with metastatic melanoma predominantly localized in the skin were randomly assigned to treatment with BCG given either intralesionally (IL-BCG) or by multiple puncture vaccination at a nontumor bearing site in the skin (MPV-BCG). Half the patients with IL-BCG experienced moderate fever, chills and malaise, suggesting systemic exposure to this live organism. However, only three of these patients required systemic antituberculous chemotherapy and all responded to it. MPV-BCG treated patients experienced significantly less systemic toxicity. Among fully evaluable patients 45% objective response rate was seen in the IL-BCG group and a 9% response rate in the MPV-BCG group, a significant difference. The only complete responses were seen in the IL-BCG group. Among fully evaluable patients, median survival was 21.1 months in the IL-BCG group and 13.3 months in the MPV-BCG groups (NSD). No patients with pretreatment anergy to all skin tests utilized, experienced objective response to BCG.

Adult↗