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W Rolfsen

Publications and source records attributed to W Rolfsen.

30 records · Page 2Linked to original sources
PubMed1989

Specific IgE antibodies to 16 widespread mold genera in patients with suspected mold allergy.

A panel of 55 sera from patients with suspected mold allergy from two different geographic areas, A and B, was investigated for specific IgE antibodies to 16 individual molds using Phadebas RAST technique. The molds investigated were Alternaria, Cladosporium, Aspergillus, Penicillium, Mucor, and Candida from the former RAST panel and ten additional new mold genera. The RAST screening revealed that 73% of patients had specific IgE antibodies to at least one of the 16 molds. Eighteen percent were negative to the six molds of the previous RAST panel but had specific IgE antibodies to at least one of the ten new molds. The highest frequency of IgE antibodies in the A group could be assigned to Cladosporium, followed by Botrytis and Helminthosporium. In group B, sensitivity to Botrytis and Phoma showed the highest frequencies. This study revealed that the relative importance of mold genera may vary greatly between patient groups from different geographic areas. Some of the species of the expanded RAST panel seem to be at least as important as Alternaria and Cladosporium. Multiple sensitivities seem to be caused by sensitization by many mold species rather than by cross-reactivity.

Alabama↗
PubMed1987

Detection of specific IgE antibodies towards cat flea (Ctenocephalides felis felis) in patients with suspected cat allergy.

Cat flea sensitivity is considered one of the most important skin diseases in cats and dogs. Cat fleas, however, are also a growing allergen problem for humans. Cat flea-specific IgE antibodies were studied in serum samples from 70 patients with suspected cat allergy, using RAST-based techniques and the nitrocellulose immunoblotting method. Results from RAST studies, using cat and cat flea as allergosorbents, showed that 46% of the patients were RAST positive against both cat and cat flea. 9% of the patients were RAST positive only against the cat flea. The nitrocellulose immunoblotting experiments were in agreement with the RAST results showing specific IgE to cat flea. The results indicate that some cat-allergic patients have specific IgE both towards cat and cat flea but also that some of the patients with suspected cat allergy might have specific IgE towards the cat flea and not the cat. RAST-inhibition and immunoblotting experiments also indicate that the allergen composition of cat flea extract differs from that of cat extract, even if common allergens have been detected, leading to cross-reactivity in some sera.

Allergens↗
PubMed1987

[Determination of specific IgE against 16 widespread mould genera. Improvement of the efficacy of the diagnosis of allergy to moulds].

Serum samples from 55 Scandinavian and US patients with a clinical history of mould allergy were screened for specific IgE Ab against 16 different moulds, the 6 mould in the Phadebas RAST panel and 10 new moulds. The studies were performed using RAST-based techniques and the nitrocellulose immunoblotting method. The RAST screening of the patient panel, revealed that 42 patients (76%) had specific IgE to at least one of the 16 moulds. The two most frequent moulds were Rhizopus and Botrytis positive in 29 patients (53%) and 27 patients (42%), respectively. Top three in terms of frequency in the US patient group were: Rhizopus greater than Botrytis greater than Phoma and in the Scandinavian group: Cladosporium greater than Botrytis greater than Helminthosporium. 15 patients (27%) were negative against the moulds in the Phadebas RAST panel, but had specific IgE against one or more of the 10 new moulds. IgE Ab concentrations measured with different genera showed different degrees of positive concordance, e.g. Botrytis-Helminthosporium (79%) and Alternaria-Rhizopus (38%), indicating complex patterns of crossreacting and genus specific allergens. This was confirmed by immunoblotting with 22 (greater than 1 PRU/ml) of the 55 sera showing up to 25 IgE binding components. The results strongly suggest that the importance of some mould genera has been underestimated as allergens.

Antibody Specificity↗
PubMed1986

Detection of allergens in mould and mite preparations by a nitrocellulose electroblotting technique.

The allergen composition of moulds (Alternaria alternata and Aspergillus fumigatus) and mites (Dermatophagoides pteronyssinus and Dermatophagoides farinae) was studied by electroblotting. The allergens were separated by SDS-gPAGE and transferred to a nitrocellulose membrane by electroblotting. Specific IgE antibodies directed against mould and mite allergens were utilized as probes to detect the allergens. The bands were localized on the nitrocellulose membrane by means of enzyme- or isotope-labelled antibody. Circulating specific IgE antibodies in mould and mite sensitive patient sera were also analyzed and characterized with respect to their IgE specificity. Under appropriate experimental conditions this electroblotting technique could also be used for quantitation of individual allergens by scanning the isotope- or enzyme-stained nitrocellulose strips.

Allergens↗
PubMed1985

Characterization of allergens and patient sera by a nitrocellulose immunoprint technique.

A rapid and convenient protein-gel blot technique for qualitative detection of antigens/allergens in pollen allergen extracts and IgE/IgG antibodies in patient sera has been developed. The antigens were separated by isoelectric focusing in agarose gel and transferred to nitrocellulose by capillary migration. After incubation of the nitrocellulose strips with serum from allergic patients, the binding of the patient's specific IgE or IgG antibodies was analyzed by using isotope-labelled or enzyme-labelled anti-IgE or anti-IgG. The time needed for detection with isotope-labelled antibody was approximately 20 h and with enzyme-labelled antibody 2 h. The immunoprint technique is easy to use, which renders it suitable for routine use in allergy research and quality control.

Allergens↗
PubMedDate not supplied

Effect of diclofenac on prostaglandin liberation in the rabbit after photorefractive keratectomy.

BACKGROUND: An analyses of the concentration of arachidonic acid metabolites and the infiltration of leukocytes in the aqueous humour of rabbits after excimer laser keratectomy was performed. The ability of topical diclofenac to block prostaglandin E2 liberation into the aqueous humour was also investigated. METHODS: Photorefractive keratectomy was performed on both eyes of 60 New Zealand rabbits. Prostaglandin E2, 6-keto prostaglandin F1 alpha and thromboxane B2 were detected by radioimmunoassay and leukocytes were counted in the aqueous humour for 120 hours postoperatively. Topical diclofenac was instilled (1 drop/4h) in six animals and prostaglandin E2 was analyzed in the aqueous humour 16 hours postoperatively. RESULTS: The prostaglandin E2 concentration in the aqueous humour increased from 13 in control animals, to 251 pg/100 microliters at 16 hours after treatment (p < 0.05). A statistically significant elevation in the aqueous concentration of 6-keto prostaglandin F1 alpha could not be demonstrated. Thromboxane B2 and leukocytes could not be detected at any time point. Topical diclofenac (1 drop/4h) reduced the levels of prostaglandin E2 in the aqueous humour below 10 pg/100 microliters, 16 hours postoperatively. CONCLUSION: Topical diclofenac (1 drop/4h) inhibited prostaglandin E2 release into the aqueous humour.

6-Ketoprostaglandin F1 alpha↗