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Biomedical subjects

W Rotzsch

Publications and source records attributed to W Rotzsch.

At least 91 records · Page 5Linked to original sources
PubMedDate not supplied

[Age-dependent changes in glucocorticoid receptors in rat liver following functional loading in in-vivo and in-vitro systems].

In aging the transformation, nuclei binding, and recycling of glucocorticoid receptor complexes (GRC) seem to be retarded or disturbed, as shown in the rat liver after stress and in an in vitro-system. Reasons of the retarded in vitro-transformation and DNA-binding could be changed properties of the receptor protein or of cytosolic modulators, in which the tested heat-stable factor f is not responsible for the aging changes. The observed aging differences, when studying the nuclei binding after Ca2+ addition, result in the question, to what extent phosphorylation/dephosphorylation processes play a role in transformation and nuclei binding of GRC.

Aging↗
PubMedDate not supplied

[Lipogenic enzymes of the lung in relation to age].

The activities of lipogenic enzymes of the lung of female rats of the Wistar strain were measured in the age groups 3, 18, 21, 25, and 30 months. The activities of malic enzyme (EC. 1.1.1.40) and citrate cleavage enzyme (EC. 4.1.3.8) decrease in dependence on aging. In contrast, the enzymes of pentose phosphate shuttle glucose-6-phosphate dehydrogenase (EC. 1.1.1.49) and 6-phosphogluconate dehydrogenase (EC. 1.1.1.44) do not show an age dependence.

ATP Citrate (pro-S)-Lyase↗
PubMedDate not supplied

Lipoprotein levels of rural and urban persons of Ethiopia in dependence on age.

The levels of serum total cholesterol, LDL- and HDL-cholesterol, triglycerides and apolipoprotein B were determined in urban and rural persons of Ethiopia in dependency on age. Regarding total and LDL cholesterol a comparable age-dependency as in the population of other countries is observed. On the other hand higher values of these parameters were found in the serum of urban persons in comparison with rural subjects which may be a reflection of the different life style.

Adult↗
PubMedDate not supplied

[Lipogenesis, aging and hormonal regulation].

Thyroid hormones stimulate hepatic synthesis of fatty acids as well as activities of lipogenic enzymes. According to the present study, there also partially exists an age dependency. In livers of 3- and 18-month-old rats of the Wistar strain both the velocity of fatty acid synthesis and the activities of lipogenic enzymes were measured in dependence on thyroid function. An impaired stimulation of malic enzyme activity under hyperthyreosis conditions was found in the older animals. The velocity of fatty acid synthesis was diminished in the group of 18-month-old rats, but there was no age dependence with respect of the effect of a variation in thyroid status. In the adipose tissue of the older animals, the activities of lipogenic enzymes were lowered. In this tissue no effects of thyreohormones in either young or old rats were observed.

ATP Citrate (pro-S)-Lyase↗
PubMedDate not supplied

[Lipogenesis, aging and hormonal regulation].

Thyroid hormones stimulate hepatic synthesis of fatty acids, as well as the activities of lipogenic enzymes. According to the present study, an age-dependence factor is also partially present. In livers of 3- and 18-month-old rats of the Wistar strain, both the velocity of fatty acid synthesis and the activities of lipogenic enzymes were measured in dependence on thyroid function. An impaired stimulation of malic enzyme activity under the conditions of hyperthyreosis was found in the older animals. The rate of fatty acid synthesis was diminished in the group of 18-month-old rats, but there was no age-dependence in respect of the effect of a variation in thyroid status. In adipose tissue of older animals, the activities of lipogenic enzymes were lowered. In this tissue no effects of thyroid hormones in both young and old rats were observed.

ATP Citrate (pro-S)-Lyase↗
PubMedDate not supplied

[Effect of thyroid hormones on lipogenic liver enzymes in relation to age].

The influence of the thyrohormone status and of a nutrition rich in cholesterol on the hepatic enzymes glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, malate enzyme, and citrate cleavage enzyme was examined in female Wistar rats aged 3 and 18 months. Contrary to the findings in young animals, hyperthyreosis does not cause any significant increase in the activities of the malate enzyme and of the citrate cleavage enzyme. A nutrition rich in cholesterol induces a decrease of the activity of the malate enzyme in 3-month old rats; not, however, in the old rats.

ATP Citrate (pro-S)-Lyase↗
PubMedDate not supplied

[Biologic aging. 24. Circadian rhythm of lipid metabolism parameters in relation to age].

The age dependence of hepatic fatty acid synthesis and of the activities of lipogenic enzymes of the liver and of adipose tissue of Wistar-rats was investigated over the 24 hours period. The data were analyzed according to the so-called "cosinor procedure" for detecting circadian rhythms and for objectively describing their parameters. In the age groups 3 and 18 months hepatic fatty acid synthesis and the lipogenic liver enzymes glucose-6-phosphate dehydrogenase, 6-phospho-gluconate dehydrogenase and malic enzyme show circadian rhythms with maximum values in the early dark period. In adipose tissue of old rats the enzyme activities are lowered and no significant circadian rhythms for glucose-6-phosphate dehydrogenase and malic enzyme are detectable.

Adipose Tissue↗
PubMedDate not supplied

[Biologic aging. 22. Oxygen and aging].

By means of the experimental-gerontological literature the question of causality between oxygen and ageing and the age-associated alterations, influencing oxygen utilisation, were discussed. Under normal conditions there are no significant decreases of high-energy compounds in the most organs, although under a strain of organism it is possible to show a reduction of adaptation and regulation on different levels. Oxygen deficiency as a primary cause of ageing could not be showed experimentally. On the other hand the classification of free radicals (= the products of oxygen activation) as primary factors of ageing are more conceivable. The estimation of causal relations is difficult because of the interlocking of physiological and pathological processes of ageing.

Adenosine Triphosphate↗
PubMedDate not supplied

[Age dependent changes in signal transduction of glucocorticoid hormones after physiological stress in liver cells of male Wistar rats].

After physiological stress older male Wistar rats showed a faster normalization of the corticosterone level in serum and of the concentration of unoccupied glucocorticoid receptors in liver cytosol than young ones, which is contrary to female rats. The nuclei binding of glucocorticoid receptor complexes (GRC) was significantly reduced in older control animals compared with young ones. The nuclei binding of GRC was reduced in young rats until 60 min after stress compared to controls, whereas it had been increasing in older animals at this time. Contrary to female rats, the addition of the endogenous ATP-stimulated translocation promoter (ASTP) alone or together with ATP did not elevate the nuclei binding, but it groups. Crossing experiments using nuclei of controls and GRC of control and stressed animals, resp., seem to disclose a longer association of GRC with the nuclei in old animals than in young ones.

Aging↗
PubMedDate not supplied

[Effect of cytoplasmic factors on cell nucleus binding of glucocorticoid receptor complexes in the rat liver of two age groups].

The nuclei binding of activated glucocorticoid receptor complexes (GRC) was significantly diminished in old, unstressed rats compared with young ones. In animals killed at different times after physiological stress there were no significant age-dependent differences in nuclei binding of GRC, which recurred in the cytoplasm after 60, 120 and 180 min, resp. Addition of Ca2+ and ATP, resp., to the incubation system increased the nuclei bound part of GRC, especially in young animals. ATP-stimulated translocation promoter (ASTP), isolated from rat liver cytosol, raised the nuclei binding of GRC in both age groups, whereby the increase was only significant in young animals. In cytosol of old rats ASTP were found in a lower concentration; moreover, the reaction between ASTP, GRC, and the template seems to be impaired.

Adenosine Triphosphate↗