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Biomedical subjects

W Rowiński

Publications and source records attributed to W Rowiński.

At least 73 records · Page 4Linked to original sources
PubMed1994

In situ perfusion and UW solution used for storage did not decrease the incidence of ATN in kidneys harvested from hemodynamically unstable donors.

The incidence of acute tubular necrosis ATN after cadaveric kidney transplantation in our centre has been in the range of 50%. A prospective study was carried out in 1991 and 1992 to assess the effect of in situ perfusion and hypothermic storage of kidneys harvested from brain-dead haemodynamically stable and unstable donors. Three litres of Ringer's solution were used for in situ perfusion. In 40 cases, the kidneys were stored in Euro-Collins (EC) solution and in the other 78 cases, in University of Wisconsin (UW) solution. Among the factors that could contribute to ATN, we analysed warm ischaemia time, anastomosis time and cold storage time. Function was considered to be delayed if the patient required posttransplantation dialysis. The donors were considered haemodynamically unstable when hypotension before harvesting was present (BP < 70 mm Hg over 2 h) despite high doses (> 15 microg/kg per minute) of dopamine or when cardiac arrest occurred at the time of harvesting and oliguria had been present for at least 2 h. Haemodynamically stable donors with a BP greater than 80 mm Hg had a normal diuresis. In all donors in this group the dose of dopamine was lower than 10 microg/kg per minute. The study showed that storage in UW solution did not influence the incidence of ATN in kidneys harvested from haemodynamically unstable donors. Differences observed in our study were due to haemodynamic status preceding donor nephrectomy and length of cold storage time.

Adenosine↗
PubMed1994

Systemic streptokinase infusion for declotting of hemodialysis arteriovenous fistulas.

In 17 out of 29 hospitalized patients (58.6%) with internal arteriovenous fistula (AVF) thrombosis a systemic streptokinase infusion was used as an alternative to urgent surgical declotting. In the remaining 12 patients (41.4%) fibrinolytic treatment was contraindicated due to the necessity for immediate dialysis, uncontrolled hypertension, active peptic ulcer, known multilevel stenoses of the fistula, or operation 8 days prior to the thrombosis. The systemic streptokinase therapy alone was successful in 9 of 17 patients treated (52.9%), 5 of 17 patients (29.4%) needed the combined therapy (streptokinase plus surgery) and in 3 of 17 patients (17.6%) the fibrinolytic therapy was unsuccessful. No serious complications attributable to the streptokinase infusion were observed. Systemic streptokinase treatment for acute AVF declotting followed by the radiological evaluation of the vessels can be a reasonable alternative to 'blind' surgical emergency reconstruction. The method makes it possible to identify those underlying anatomic abnormalities of the draining vein which may be localized at some distance from the anastomosis and so overlooked during surgery.

Adult↗
PubMed1994

Experimental assessment of canine pancreatic segment function.

The purpose of the study was to examine the variability of pancreatic arterial vascularization and to evaluate a vascular model of canine pancreatic segment for transplantation, providing preservation of normal endocrine and exocrine functions. The study included the assessment of pancreatic segment function on the basis of glycemia and post mortem corrosion-casts dissection.

Animals↗
PubMed1991

The effect of moderate operative trauma on the blood lymphocyte reactivity--immunomodulatory effect of interleukin-2.

The influence of moderate operative trauma on lymphocyte responsiveness to phytohemagglutin (PHA) and in autologous mixed lymphocyte response (AMLR) was studied. Furthermore, production of interleukin 2 (IL2) and immunomodulatory effect of exogenous IL2 on lymphocyte reactivity after surgery were examined. The responsiveness to PHA declined on day 1 (p less than 0.05) and the level of AMLR was found decreased on days 1 (p less than 0.05), 3 (p less than 0.02) and 5 (p less than 0.03) after surgery. Production of IL2 significantly decreased on day 1, 3 and 5 (p less than 0.05) after operation. Supplementation of PHA and AMLR cultures with exogenous IL2 restored the attenuated postoperative responsiveness to the levels observed preoperatively. This observation may justify IL2 therapy in surgical patients with high risk of uncontrollable postoperative infections.

Adjuvants, Immunologic↗
PubMed1989

The radiological examination of the pancreas harvested for transplantation.

The radiological examination of the pancreatic arterial blood supply was performed to show the presence of the arterial variability. 40 arteriograms of pancreatic vessels were made. The research confirmed the subsistence of the arterial variability concerning the dorsal pancreatic artery in particular. The importance of the previous arteriography in donor, before harvesting the pancreatic graft, was unterlined.

Angiography↗
PubMed1989

Prolongation of heart allograft survival in course of donor specific antigen and alloserum treatment in the rat.

Different cell subpopulations involved in the induction of immune enhancement of the heart graft survival in rats differing across the major histocompatibility barrier has been studied. Cells devoid of class II antigens on their surface, e.g. erythrocytes, platelets, and thymocytes have been found ineffective, whereas subpopulations rich in class II antigens were found to be highly effective in induction of specific unresponsiveness when combined with donor specific alloserum. Our results suggest that class II antigens take part not only in induction of rejection response but may be also responsible for generation of specific unresponsiveness towards donor antigens.

Animals↗
PubMed1983

Immunomodulating influence of active metabolite of cyclophosphamide on human lymphocyte immunoglobulin biosynthesis in vitro.

4-hydroxycyclophosphamide (4HCy) inhibits T cell-dependent and direct B cell humoral responses of human lymphocytes in vitro, but causes a potentiation of the former in the lowest drug concentrations. Lymph node humoral responses are strongly sensitive, but those of spleen are relatively resistant to the drug. Although the induction of suppressor T cells is Cy-resistant, the expression of their function is sensitive. The suppressor cell function of antibody production appears to be more sensitive than the helper function.

Adjuvants, Immunologic↗
PubMed1981

Laboratory-scale procedure for preparation of horse anti-dog lymphocyte globulin (HADLG).

Procedure of preparation of horse anti-dog lymphocyte globulin on laboratory scale is described. Adsorption of antibodies against dog plasma proteins was performed with the use of immunosorbent prepared by coupling dog plasma proteins to aluminium hydroxide gel. Anti-lymphocyte immunoglobulins G and T were purified using two-step fractionation. Addition of glycine to the purified immunoglobulins prevented the aggregate formation. Purified immunoglobulins were tested in dogs receiving kidney allografts. The short term administration of HADLG significantly prolonged the time of survival of allografted kidney in comparison to the untreated controls.

Animals↗
PubMed1979

Factors responsible for the functional pattern of cadaveric kidneys stored up to 25 hours before transplantation.

The functional outcome after the transplantation of 97 cadaveric kidneys stored up to 25 hours with simple hypothermia was analysed in relation to age of donor, extent of pre-agonal kidney damage, the length of warm, cold and standardised ischaemic times and the presence of multiple renal arteries. Analysis of various factors showed that the most important for function after transplantation were pre-agonal anoxic damage and the ratio of the cold/warm ischaemia time. Based on that observation, a diagram has been proposed which allows calculation of the safe cold storage time after retrieval of the kidney.

Adolescent↗