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Biomedical subjects

W S Jiang

Publications and source records attributed to W S Jiang.

6 recordsLinked to original sources
PubMed2001

Nitric oxide as a regulator in preimplantation embryo development and apoptosis.

OBJECTIVE: To investigate the mechanisms of nitric oxide (NO) in the development and apoptosis of preimplantation mouse embryos. DESIGN: Prospective, controlled study. SETTING: Medical college laboratory. SUBJECT(S): Two-cell embryos from outbred ICR mice. INTERVENTION(S): Hyperstimulation protocol, two-cell embryos were collected, then treated with or without an NO synthase inhibitor (L-NAME) or an NO donor (SNP) and combined with a cGMP analogue (8-Br-cGMP) or a selective inhibitor of NO-sensitive soluble guanylyl cyclase (ODQ). MAIN OUTCOME MEASURE(S): The development of ICR mouse embryo from two cells to blastocyst stages in vitro. RESULT(S): The development of blastocyst was inhibited by L-NAME in a concentration-dependent manner (0.1-10 microM) and 0.1 microM SNP reversed this effect (80.5% of control). Annexin-V/propidium iodide and terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling techniques demonstrated that excessive NO (> or =10 microM) might induce apoptosis in the mouse embryos. 8-Br-cGMP reversed the inhibitory effect of L-NAME and rescued the embryo growth. ODQ inhibited the embryo development in a dose-responsive fashion (0.1--100 microM) but had no effect in the NO-induced embryo apoptosis. P53 and Bax were found to be up-regulated during the embryo fragmentation. CONCLUSION(S): These results indicate that the cGMP pathway might be involved in the NO-regulated embryonic development, but not in NO-induced apoptosis, for which P53/Bax pathway might be involved.

Animals↗
PubMed2001

Uptake and accumulation of copper by roots and shoots of maize (Zea mays L.).

The effects of different concentrations of copper sulfate on root and shoot growth of maize(Zea mays L.) and the uptake and accumulation of Cu2+ by its roots and shoots were investigated in the present study. The concentrations of copper sulfate (CuSO4 x 5H2O) used were in the range of 10(-5)-10(-3) mol/L. Root growth decreased progressively with increasing concentration of Cu2+ in solution. The seedlings exposed to 10(-3) mol/L Cu2+ exhibited substantial growth reduction, yielding only 68% of the root length of the control. The shoot growth of the seedlings grown at 10(-5)-10(-4) mol/L Cu2+ were more or less the same as the control seedlings. The leaves treated with 10(-3) mol/L Cu2+ were obviously inhibited in shoot growth. The fresh and dry weights both in roots and shots decreased progressively with increasing Cu2+ concentration. This fits well with the above mentioned effects of copper sulfate on root growth. Zea mays has considerable ability to remove Cu from solutions and accumulate it. The Cu content in roots of Z. mays increased with increasing solution concentration of Cu2+. The amount of Cu in roots of plants treated with 10(-3), 10(-4) and 10(-5) mol/L Cu2+ were 10, 8 and 1.5 fold, respectively, greater than that of roots of control plant. However, the plants transported and concentrated only a small amount of Cu in their shoots.

Copper↗
PubMed1999

[The antagonistic effect of naloxone on the epidermal conductivity of amphibian embryos].

The immunocytological work revealed that in amphibia beta-endorphin is distributed in embryonic epidermis during development with prominent expression at late tail bud stage which coincides with the stage of epidermal conductivity of full strength. To see whether any relationship exists between the presence of beta-endorphin and the epidermal conductivity of amphibian embryos, naloxone of 1 and 2 mumol/L was used. It has been demonstrated that after 45-60 minutes in the naloxone solution the embryonic epidermal conductivity disappeared and 90-120 minutes after the beginning of naloxone treatment the epidermal conductivity was again recovered. It was suggested that the opiate peptides, such as beta-endorphin, play some role in the epidermal conductivity of amphibian embryos.

Animals↗
PubMed1995

[Experimental and clinical study of shuanghuanglian aerosol in treating acute respiratory tract infection].

Two hundrde and two cases of acute respiratory tract infection (ARI) were treated with Shuanghuanglian (SHL) aerosol. In these cases, the majority of cases were virus infection and 64% of them was caused by the respiratory syncytial virus (RSV). The virostatic and bacteriostatic test were done in vitro by the cell culture method and it was shown that SHL could inhibit the RSV, para-influenza I-IV and 23 kinds of pathogenic bacteria such as Staphylococcus aureus etc. The bacteriostatic effect was positively correlated to the SHL concentration. Experimental study showed that SHL could enhance the NK cell activity, promote the production of alpha-interferon and raise the rate of lymphocyte transformation. The controlled observation on SHL preparation with various dosage-forms revealed that the SHL aerosol in treating early ARI showed better results than that of injections and oral liquor symptomatologically (P < 0.01). The effective rate was 96%.

Acute Disease↗