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W S REHM

Publications and source records attributed to W S REHM.

At least 19 recordsLinked to original sources
PubMed1959

The concentration of glucose in mammalian liver.

Data on barium-zinc filtrates of liver homogenates and of boiled liver indicate that the free glucose content of liver exceeds the blood glucose level. For instance, for boiled liver, the glucose level is 10.1, compared with a blood level of 5.4 mM/kg. Method of preparation of the tissue is important for the interpretation of the final results, as has been shown in appropriate control experiments. Various methods including paper chromatography were used to show that the reducing substance in liver is glucose. The relation of the high glucose content of liver to hexokinase activity, phosphate activity, and to glucose transport between liver cell and blood is discussed.

Animals↗
PubMed1956

The freezing point depression of mammalian tissues in relation to the question of osmotic activity of cell fluid.

The freezing point depression of freshly excised frozen tissues, pulverized in a hydraulic press or in a mortar, is greater than that of plasma. Even at 0 degrees C. the freezing point depression of such homogenates increases significantly with time. Dilution data indicate that such freezing point data are valid. The presence of intact cells has been shown in smears of tissues pulverized in a mortar, but not in smears of those crushed in a hydraulic press. The osmolarity of various diluent solutions affects the calculated osmotic activity of tissue homogenates presumably because of delayed diffusion between the diluent and cell fluid. With a hypertonic NaCl diluent, spuriously low values of tissue osmotic activity are found from calculations assuming instantaneous mixing between homogenates and diluents. The limitations of data from cryoscopic experiments and from tissue-swelling experiments are discussed in relation to the basic question of whether or not cell fluid is isotonic to extracellular fluid.

Animals↗