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Biomedical subjects

W Sadowski

Publications and source records attributed to W Sadowski.

At least 19 recordsLinked to original sources
PubMed1991

[Temperature-sensitive clones of herpes simplex virus type 1 from laboratory and clinical strains. I. Cloning and basic pathogenetic and immunogenic properties].

Two HSV-1 strains were used in the study: McIntyre laboratory strain and "eye" strain isolated from a patient. Temperature-sensitive clone of HSV-1 was isolated from McIntyre strain as a consequence of virus replication carried out at lowered temperature (28 degrees C). Temperature-resistant clones were obtained from both strains through passages at 39 degrees C and through heating for four times at 45 degrees C. Pathogenic properties of the temperature clones obtained were determined in inbred mice Balb/c and CFw/Pzh. A loss of pathogenicity for mice of temperature-sensitive clone and an increase of pathogenicity of temperature-resistant clones were noted as compared to parental strains. It was found that an introduction of temperature-sensitive clone, with lowered virulence immunizes against highly virulent temperature-resistant clone.

Animals↗
PubMed1991

Preparation of antibacterial and antitoxic Clostridium difficile sera.

Preparation of Clostridium difficile antibacterial and antitoxic sera is presented. Fifty one strains (72%) were typeable within Delmee scheme. Twenty strains (28%) belonged to new Polish serogroups designated 18, 27, 70, 71, 72, 88, 89 and NICH. Supernatants of all toxigenic Clostridium difficile strains were neutralized by gamma-globulin fraction of goat Clostridium difficile antitoxin in neutralization assay when it was performed on McCoy cell line. Only 8 toxigenic strains (21%) were positive in counterimmunoelectrophoresis.

Animals↗
PubMed1990

[Studies of the adaptation of influenza viruses to lowered temperatures of replication. II. Studies in vivo].

Swiss white mice were given intranasally suspension of influenza A virus (H3N2) isolated at different period of time and replicated in lowered temperatures in 11 days old chicken embryos. The presence of antigen in lung of animals was detected by IF. They were given the virus replicated at 30 degrees C at different rate depending on strain tested. No distinct differences were observed in haemagglutination inhibition antibody level. On the other hand the level of neuraminidase activity inhibiting antibodies level was significantly higher after giving virus replicated at 30 degrees C than after giving the virus replicated at 37 degrees C. In the case of epidemic strains 4-5 fold fold increase of immunogenicity of neuraminidase component was observed and in the remaining strains immunogenicity of neuraminidase increased 1-5-fold only.

Adaptation, Physiological↗
PubMed1990

[Antiviral activity of commercial immunoglobulin preparations].

Immunoglobulin preparations for intravenous use of five different firms--Biotest, Hoechst, Merieux, Sandoz, WWSS--were used for the study. Antibody level for Epstein-Barr, cytomegalia, herpes simplex, varicella-zoster and measles viruses was determined in these preparations stored at 4 degrees C and in order to determine their stability they were tested after incubation at 37 degrees C and 61 degrees C. The influence of immunoglobulin (Bioglobulin and Sandoglobulin) on mouse survival infected with HSV-1 was determined. Results of serological studies revealed differentiated antibody level for particular virus antigens both in various series of a given preparation as well as between immunoglobulins of different producers. Protective activity of immunoglobulin was mainly found when given 24 hours before challenge with HSV-1. This was the case not only when preparations stored at 4 degrees C were given but also for those which were incubated at 37 degrees C for months. Forty percent higher rate of survival of mice as compared to control group was seen when immunoglobulin were given 8 hours after infection.

Animals↗
PubMed1990

[Growth of Coxiella burnetii in selected cell cultures].

Usefulness of monolayer cell cultures for C. burnetii multiplication was evaluated. Continuous Vero cell line and primary cell (cultures cell culture of monkey kidney Cercopithecus aethiops, cell culture of chicken fibroblasts, diploid cell line of human embryo) was inoculated for this purpose. Two Coxiella burnetii strains suspensions were used as inoculum of standard Henzerling strain and Zamość strain isolated from an area of Poland. Primary cell lines were characterized by considerable degree of sensitivity to, C. burnetii infection, and at the same time, the most sensitive was the line derived from monkey kidney. Progressive vacuolisation of infected culture cells was designated as cytopathic effect. Henzerling strain showed higher infectivity (4.7 x 10(8) PFU/ml) for chicken fibroblasts as compared to Zamość strain (8.7 x 10(6) PFU/ml). On the other hand both strains induced cytopathic effect in Vero cell line without degeneration of host cells.

Animals↗
PubMed1987

HSV-1 infection and immunity in immunosuppressed mice.

The effect of cyclophosphamide (CY) and hydrocortisone (HY) on the susceptibility of mice to intracerebral and intraperitoneal infection with herpes simplex virus type 1 (HSV-1) was investigated. The mean survival time, the survival ration, the localization of HSV-1 antigen in brain, spleen and liver as well as immunity after immunization with inactivated virus were determined. In the case of primary infection, an increased susceptibility to HSV-1 was observed after administration of the immunosuppressive drug. Immunization increased the resistance of mice to virus challenge, but no such effect was observed when the virus challenge, but no such effect was observed when or HY. The influence of CY and HY on the immunization process itself was divergent: when HY was given at the time of immunization, the resistance to virus challenge was abolished. On the other hand, CY given simultaneously with inactivated HSV-1 did not depress the immunization effect.

Animals↗