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Biomedical subjects

W Sawicki

Publications and source records attributed to W Sawicki.

At least 19 recordsLinked to original sources
PubMed2001

First cases of acute human granulocytic ehrlichiosis in Poland.

The first three cases of acute human granulocytic ehrlichiosis in Poland are described. Blood samples were tested by an indirect immunofluorescence method to detect specific serum antibodies, and the polymerase chain reaction was used to detect ehrlichial DNA. Additionally, peripheral blood smears were examined for the presence of morulae. According to criteria of the Centers for Disease Control and Prevention, all three cases can be classified as confirmed granulocytic ehrlichiosis. Using the criteria recommended by a consensus group, however, two cases can be classified as confirmed granulocytic ehrlichiosis and one case as probable granulocytic ehrlichiosis.

Acute Disease↗
PubMed2001

Penetration of verapamil hydrochloride in the presence of sodium glycocholate as penetration enhancer through mucous membrane.

A model buccal drug formulation (BDF) with verapamil hydrochloride (VH) is in the form of a thin elastic disk made of two layers. The aim of this paper was to determine the influence of sodium glycocholate (SG) as a penetration enhancer in a BDF on the in vitro penetration rate of VH through pig oesophageal mucosa. It was found that VH penetrates mucosa. In 6 h 10.35 mg/cm2 of drug diffused from saturated solution in a buffer of pH 5.3 white from diffused only 7.78 mg/cm2 and 4.68 mg/cm2 of VH, respectively saturated buffers of pH 6.8 and 7.3. In 6 h approximately 1.3 mg/cm2 of VH penetrate the mucosa from the control BDF model which does not contain the penetration enhancer. Conversely the content of SG in the model BDF contributes to the increase in drug penetration rate.

Administration, Buccal↗
PubMed2000

Cyclodextrin enhances spermicidal effects of magainin-2-amide.

Magainins are antimicrobial peptides with known spermicidal activity. Their activity is inhibited by cholesterol present in eukaryotic cell membranes. Pretreatment of spermatozoa with methyl-beta-cyclodextrin, which extracts cholesterol from cell membranes and induces capacitation, sensitizes them to magainin-2-amide as shown by a decrease in human sperm motility determined by computer-assisted sperm analysis and a concomitant decrease in sperm viability, as measured by MitoTracker(R) Red CMXRos labeling. Magainin-2-amide affects mainly the fast progressive spermatozoa inducing them directly into an immotile state, without an increase in motile non-progressive and slow progressive spermatozoa. We conclude that methyl-beta-cyclodextrin highly potentiates the deleterious effect of magainin-2-amide on human spermatozoa. Most probably, this effect can be explained by cholesterol extraction from sperm cell membranes.

Animals↗
PubMed1998

Fractional curettage, hysteroscopy and imaging techniques: transvaginal sonography (TVS), magnetic resonance imaging (MRI) and computed tomography (CT) in the diagnosis of cervical canal involvement in cases of endometrial carcinoma.

The value of preoperative diagnostic procedures (FC, hysteroscopy, TVS, MRI, CT) used to evaluate cervical canal involvement in cases of endometrial carcinoma was assessed. The results of these diagnostic methods were compared to postoperative pathologic examination results. Evaluation of the cervix by CT was limited. High NPV of the rest of the methods together with their negative results allowed us to exclude cervical infiltration with a high probability. Alas, none of them was adequate to confirm the presence of cervical involvement because of their low PPV which ranged from 8.3% (hysteroscopy) to 50% (MRI). Among all these diagnostic procedures TVS was relatively efficient (77.8%), specific (78%), sensitive (75%) and inexpensive. This method is preferable in the evaluation of possible cervical infiltration.

Adenocarcinoma↗
PubMed1998

[Preoperative evaluation of the invasiveness of myometrial and pelvic lymph nodes in patients with endometrial cancer].

Transvaginal sonography (TVS), computed tomography (CT) and magnetic resonance imaging (MRI) were used for preoperative diagnosis of myometrial and pelvic lymph nodes invasion in patients with endometrial cancer. Results of examinations were compared with histopathological findings. Sensitivity, specificity, efficacy, positive and negative prognostic values of these imaging techniques were compared. TVS and MRI had similar efficacy in the evaluation of depth of myometrial invasion (78.8% vs 67.6%). In the evaluation of deep invasion TVS in spite of lower sensitivity had higher specificity and positive prognostic value. Efficacy of all methods for evaluation of lymph nodes metastases was similar (80-82%), but TVS had higher positive prognostic value than MRI. CT in comparison with TVS and MRI was less useful in the diagnosis of myometrial invasion.

Endometrial Neoplasms↗
PubMed1998

[Preoperative evaluation of cervical involvement in endometrial cancer].

Usefulness of fractionated curettage, histeroscopy, transvaginal sonography and MR-imaging in the preoperative diagnosis of cervical involvement of endometrial cancer was studied. Sensitivity, specificity, efficacy, positive and negative prognostic value of above-mentioned examinations were compared. None of examinations was adequate for diagnosis of presence of cervical invasion because of their low positive prognostic values. Negative prognostic value of all examinations was high. MR-imaging and transvaginal sonography had the highest efficacy and comparatively high sensitivity and specificity. Authors suggest, that transvaginal sonography--considering its simplicity and relative cheapness--should be obligatory in the diagnosis of endometrial cancer advancement.

Cervix Uteri↗
PubMed1998

[Evaluation of research--reality and illusions].

Productivity and effectiveness of research are defined and their indicators discussed. Particularly, citation index (CI) and impact factor (IF) as the measures of research quality are analysed. Citation index despite its drawbacks and pitfalls is the best, among applying at present, measure of research quality. Impact factor due to its severe drawbacks is not recommended as a measure of research quality, particularly for the matching. The mixture of criteria excluding IF are propose to evaluate research quality.

Bibliometrics↗
PubMed1995

Hysteroscopy with selective endometrial sampling after unsuccessful dilatation and curettage in diagnosis of symptomatic endometrial cancer and endometrial hyperplasias.

We report the incidence of endometrial hyperplasia and endometrial cancer recognised by hysteroscopy in 202 patients with recurrent abnormal uterine bleeding. In all these women previously performed dilatation and curettage was unsuccessful. Endoscopy connected with direct biopsy allowed us to recognise in 26 (12.9%) patients pathological endometrial lesions in 19 of cases endometrial hyperplasia (with or without cell atypia) and in 7 patients endometrial cancer. The hysterosocpically recognised abnormalities in more than 70% were focal. Hysteroscopy with selective endometrial biopsy, performed after unsuccessful curettage is an essential tool in the diagnosis of recurrent abnormal uterine bleeding caused by pathological endometrial lesions.

Adult↗
PubMed1995

Transvaginal Doppler ultrasound with colour flow imaging in benign and malignant ovarian lesions.

Since early detection of ovarian cancer is difficult, most cases are diagnosed at advanced stages. The imaging diagnosis is based on pattern classification and is limited with respect to the precise determination of malignancy. High-frequency transvaginal sonography improves the ability to detect malignant ovarian tumors over that of transabdominal route, however, the predictive values are unsatisfactory because of inability to distinguish between malignant and benign tumors that have similar morphologic characteristics. The introduction of transvaginal colour flow imaging has allowed detection of low-resistance intratumoral blood vessels, characteristic of malignant tumors, and visually reflected the state of blood flow of an ovarian tumor. These two ultrasonographic methods were used for diagnosis of ovarian tumors in 65 women treated in our Department of Obstetrics and Gynecology. Waveforms of the parenchymal tumor arteries or tumor surface arteries were compared using value of the resistance index (RI). The sensitivity, specificity and accuracy of the preoperative RI in detecting malignant ovarian tumors were 100%, 94%, 95.4% respectively. The sensitivity, specificity and accuracy of preoperative suspicious sonographic findings in detecting malignant ovarian tumors were 100%, 61% and 71%. Positive and negative predictive values of colour flow imaging were 85% and 100%, whereas for grey-scale transvaginal ultrasonography they were 46% and 100% respectively. The findings of this study suggest that transvaginal colour Doppler is a method which is superior to the other methods for preoperative evaluation of ovarian malignancy.

Adult↗
PubMed1995

[Evaluation of endometrial changes in women with cyclical mastalgia].

Cyclical mastalgia which almost 40% of women suffer from is caused by inadequate ovarian hormones secretion. There are reports regarding increased number of endometrial steroid receptors in this group of women. The possibility of higher risk of endometrial cancer development in these women is very probable. 119 women with cyclical mastalgia aged 16-55 (means age 35.4 years) were investigated. Anovulatory cycles occurred in 63%. Increased estrogen action in vaginal smears was found in 24.1%, also decreased progesterone effect had been seen in 20.3% of our cases. Serum estradiol levels were elevated in 10.6% and 39.1% of the patients had lower serum progesterone levels. Mean progesterone--estrogen luteal index value was decreased to 0.99. Irregular uterine bleedings occurred in 16.8 of women (aged 41-48 years). Histological endometrium examinations revealed changes due to insufficient progesterone action.

Adolescent↗
PubMed1993

[Cardiac arrhythmias during extracorporeal shock wave lithotripsy].

Holter monitoring was performed in 55 patients (24 women, 31 men), mean age 50.7 +/- 11.3 who underwent ECG-triggered extracorporeal shock wave lithotripsy (ESWL). Patients were divided into two groups A--30 with history of cardiac disease and B--25 control. The heart rate was significantly higher before and after ESWL in the first group. The number of premature ventricular contractions was higher during ESWL in the same group. No other cardiac complication was observed.

Adult↗
PubMed1992

[Ectopic spleen in pelvis minor--a case report].

A case of ectopic spleen diagnosed as a left adnexal tumor in a 19 year old female was presented. Considering the rareness and lack of typical symptoms, diagnosis of this pathology still is very difficult, and establish most often intraoperatively. In every case of ascertainment irregular mass in minor pelvis one should differentiate it among: colonic diverticulosis, wandering kidney, coproliths, colon and mesentery tumors and ectopic spleen. We consider that ultrasonography plays the special role in these cases. In every case of stating the pathologic structure in minor pelvis it seems advisable not to content only with estimation of genital organs, but also locate such as: kidneys, liver, spleen. Such a procedure allows to state preoperative diagnosis more precisely.

Adnexa Uteri↗
PubMed1991

[Psycho-social precursors of arterial hypertension in men and women in light of clinical cohort studies].

Following two prevalence studies of arterial hypertension carried out at 5-year interval on 6487 adult inhabitants of Ochota District, Warsaw, screening for new cases was performed at the end of the five- or ten-year observation period. Interviews were collected from 472 incident and unknown prevalence cases and 499 randomly chosen non-cases regarding social and work conditions, selected behavioral traits and personal habits. The risk of hypertension was more closely associated with blue collar social status, poor economic self-assessment and repetitive type of work in women than men. In males, but not females, higher risk was connected with expressed anger followed by feeling of guilt, but no otherwise suppressed anger was predictive of hypertension. In both sexes the incidence of the disease was higher among people reporting less life events, and with less involvement in the household and/or professional activities.

Cohort Studies↗
PubMed1991

Fourier analysis of the nuclear and cytoplasmic shapes of living two-cell murine embryos.

Living two-cell mouse embryos were flushed out from the oviduct 17, 24 and 36 hours after fertilization in order to obtain cells in the G1S, early G2 and late G2 phases of the second cell cycle. The nuclei of the living cells were stained with Hoechst 33342. The coordinates of the contour shapes of the entire cells (cellular contours) were registered by contour image processing with a TV camera coupled with a computer; the contours of the nuclei were computed by means of a digitizer coupled with the computer. Fourier analysis of the cellular and nuclear contours revealed systematic modifications in the folding of the cells and nuclei in the course of the murine second cell cycle. The progression of cells through the second cell cycle was correlated with an increasing diversification of cellular and nuclear shape, with the diversification being much more pronounced in the nuclear shapes.

Animals↗