An unexpected response of G2/S chromosomes after prior irradiation in G0 and BUdR-treatment.
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Biomedical subjects
Publications and source records attributed to W Scheid.
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Neurology and psychiatry are combined in the easily misunderstandable term "Neuropsychiatry" as equal ranking sister disciplines. Neurology with its rectilinear development and the gain in important auxiliary methods enjoys general high respect. It is quite different with psychiatry. It is tainted with the events of the National Socialist domination. Its logical therapeutic methods are associated with inhumanity. Etiology and pathogenesis of the "endogenous" psychoses are still veiled in darkness. This gap in knowledge, detrimental to the prestige of psychiatry, is filled with theories, hypotheses and speculations. An extensively popularized psychology questions some concepts of a classical psychiatry wrongly described as conservative. Such uncertainty is alive even in the ranks of psychiatrists, to the detriment of their responsibility. Psychiatry must serve the care of the individual patient, renouncing all possible temptations. Some success can be expected from the "out-patient services" but this demands professional knowledge and critical ability besides activity.
In 5-bromodeoxyuridine (BUdR)-substituted human chromsomes stained with 4'-6-diamidino-2-phenylindole (DAPI) differential staining is suppressed totally by the H+-donor cysteamine (concentration 0.08 M). We propose that differential staining appears because the double BUdR-substituted chromatid will be disintegrated via a photosensitive dye-visible light system. It is suggested that cysteamine prevents the production of strand breaks in DNA and, consequently, differential staining in BUdR-substituted chromosomes. Furthermore it is shown that differential staining with DAPI causes irreversible changes in the double BUdR-substituted chromatid. This finding can be explained with the above mentioned mechanism.
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Anaphase chromosomes exhibit two longitudinal structures which are connected at their ends in a U-shaped closed configuration. The U-shaped ends might be part of the chromosome characterized by a relatively dense condensation of chromosomal material or part of the loop-like structure of the chromosome per se.
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