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W Schnepp

Publications and source records attributed to W Schnepp.

6 recordsLinked to original sources
PubMed1997

A small region in phosducin inhibits G-protein betagamma-subunit function.

G-protein betagamma-subunits (G(betagamma)) are active transmembrane signalling components. Their function recently has been observed to be regulated by the cytosolic protein phosducin. We show here that a small fragment (amino acids 215-232) contained in the C-terminus of phosducin is sufficient for high-affinity interactions with G(betagamma). Corresponding peptides not only disrupt G(betagamma)-G(alpha) interactions, as defined by G(betagamma)-stimulated GTPase activity of alpha(o), but also other G(betagamma)-mediated functions. The NMR structure of a peptide encompassing this region shows a loop exposing the side chains of Glu223 and Tyr224, and peptides with a substitution of either of these amino acids show a complete loss of activity towards G(o). Mutation of this Tyr224 to Ala in full-length phosducin reduced the functional activity of phosducin to that of phosducin's isolated N-terminus, indicating the importance of this residue within the short, structurally defined C-terminal segment. This small peptide derived from phosducin, may represent a model of a G(betagamma) inhibitor, and illustrates the potential of small compounds to affect G(betagamma) functions.

Amino Acid Sequence↗
PubMed1997

[Perspectives of nursing science. Formation of a theory in a practice discipline].

In this article some problems about nursing theories are discussed. It is presumed that a science does not turn into a nursing science because it deals with nursing practice. Crucial is the way in which the practice is viewed, i.e. the question which theory is the starting point. Even normative nursing theories must be tested out in reality though this verification cannot be made exclusively with the methods of logical empiricism. The obligation for nursing theories to fit the metaparadigm as well as the concept of metaparadigm are discussed. It is also stated that nursing theories do not correspond to the diversity of everyday nursing. In order to create a complex everyday live, grounded theories are needed. For a better understanding, two grounded theories are described.

Diffusion of Innovation↗
PubMed1997

[Problems in the care of mentally handicapped adults in general hospitals].

The care of the mentally handicapped with their special needs and problems represents (despite written nursing theories and conceptions) a situation of stress and burden in the everyday life of a general hospital. The nursing staff organises their work with the mentally retarded often only on the basis of common sense without any specific professional knowledge. This analysis shows that a professional attitude has to deal with different phenomenon such as perception, communication, human dignity, but also with feelings of disgust when working with patients and being confronted with their problems. It is important to have basic knowledge on the mentally handicapped to understand their specific needs. This knowledge is essential in order to accept and tolerate mentally retarded people as a part of our social life.

Adult↗