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Biomedical subjects

W Slattery

Publications and source records attributed to W Slattery.

6 recordsLinked to original sources
PubMed2000

Neurofibromatosis 2 phenotypes and germ-line NF2 mutations determined by an RNA mismatch method and loss of heterozygosity analysis in NF2 schwannomas.

We used a novel RNase cleavage assay (NIRCA) to screen for neurofibromatosis 2 (NF2) mutations in NF2 schwannomas. Mutations were found in tumors in 16 of 20 patients. Eleven patients (55%) had loss of heterozygosity or loss of one allele, indicating that the mutation was a germ-line mutation. The phenotypes of these patients were consistent with previous NF2 genotype-phenotype correlation studies: patients with nonsense mutations had severe phenotypes, whereas those with splice-site or missense mutations had milder and variable phenotypes. These results confirm the utility of NIRCA as a rapid and convenient method for screening for germ-line NF2 mutations.

Adolescent↗
PubMed1999

Establishment of primary vestibular schwannoma cultures from neurofibromatosis type-2 patients.

Primary cultures were established from vestibular schwannomas of NF2 patients. The cultured tumor cells were selectively amplified by growth factor supplemented medium and characterized by immunocytochemistry. NF2 cDNA was amplified by RT-PCR and mutations were detected by both the non-isotopic RNase cleavage assay and direct DNA sequencing, no detectable wild-type NF2 transcript was found in cDNA from the cultured cells. Distinguishable morphology and growth rate differences have been observed in different passages of the primary cells. The data suggest that a pure schwannoma primary culture can be established and could be very useful in vitro model for further understanding the NF2 gene function in Schwann cells.

2',3'-Cyclic-Nucleotide Phosphodiesterases↗
PubMed1997

Marrow-mesenchyme connections in the fetal and newborn tympanum. A new entity.

Examinations of 41 human fetal, 8 infant, and 8 juvenile temporal bones prepared for light microscopic evaluation revealed direct connections between the hematopoietic bone marrow and the unresolved mesenchyme in the middle ear. The connections first appeared at 15 weeks of gestation and became bridged by fibrous tissue, in most cases, by the postpartum age of 10 months. Between 16 and 18 months after birth, the marrow-mesenchyme connections gradually disappeared. The areas in which the connections were most numerous were the anterior epitympanum, the sinus tympani medial to the stapedius muscle, and transitory bone that occupies the area that will become the aditus of the antrum. Immunohistochemical staining demonstrated the existence of mature leukocytes in these connections. These connections may help protect the middle ear against bacterial invasion during the postnatal period.

Bone Marrow↗
PubMed1981

Essential fatty acid deficiency and evidence for arachidonate synthesis in the cat.

1. There is controversy regarding the capacity of the cat to convert 18: 2 omega 6 to 20: 4 omega 6 and the ability of the essential fatty acid (EFA)-deficient cat to produce 20: 3 omega 9. 2. This paper reports the isolation and identification of 20: 3 omega 9 from kidney phospholipids of EFA-deficient cats. 3. The results suggest that the cat is capable of limited synthesis of 20: 4 omega 6 using a delta 5- and delta 8-desaturase.

Animals↗