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Biomedical subjects

W Stach

Publications and source records attributed to W Stach.

At least 55 records · Page 3Linked to original sources
PubMed1980

Innervation of the gastric wall related to acid secretion: a light and electron microscopy study on rats, rabbits and guinea pigs.

The increasing use of vagotomy for the surgical treatment of ulcers has stimulated interest in the structure and function of the gastric wall innervation. This paper presents a survey based on microphotographs (rat, rabbit, and guinea pig) of the stomach innervation. Special attention is paid to the relationship between the vagus nerve and the intramural nervous system and the ganglion cells, synaptic junctions in the gastric wall, the innervation of the mucosa and its relationship to mast and parietal cells. The functional significance of the findings is discussed: (1) the electron microscopic evidence of synaptic junctions on parietal cells shows that hydrochloric acid secretion is influenced directly by the nervous system. The nerves terminating at the parietal cells originate exclusively from the myenteric plexus where the vagus fibres already terminate without entering the mucosa. (2) The innervation of mast cells and their proximity to the parietal cells makes some indirect nervous influence on hydrochloric acid secretion likely.

Animals↗
PubMed1979

[Differentiated vascularization of Dogiel's cell types and the preferred vascularization of type I/2 cells within plexus myentericus (Auerbach) ganglia of the pig (author's transl)].

It could be proved that the typ I/2-cells are the preferred capillarized nerve cells not only in the plexus Schabadasch (Stach 1977a), but also in the plexus Auerbach. It underlines the special importance of this type of cells for the function of the nervous system of the intestinal wall. Our findings concerning the vascularisation of the intramural nerve plexuses allow to conclude that the regular blood-supply is decisively important for a normal function of the nervous system of the intestinal wall. The knowledge of these facts might be of interest for gastroenterology.

Animals↗
PubMed1979

[Innervation of the mucosa of the small intestine of laboratory animals. I. Architecture, light microscopic structure and histochemical differentiation].

1. The small intestine mucosa has a dense and differentiated innervation. The most dense nerve plexuses are contained in the villi. Each tubular gland is surrounded by a rope-ladder-like plexus, mostly developed in the lower and upper third. 2. According to our observations most of the axons in the mucosa are cholinergic. Cholinergic and adrenergic axons take part in the innervation of the glands. Moreover enterochromaffine cells can be innervated for instance sympathetically. Intraepithelial axons could not be found.

Adrenergic Fibers↗
PubMed1979

[Innervation of the small intestinal mucosa of laboratory animals. II. Ultrastructure of neuro-cellular connections].

In addition to our light microscopic contribution (Stach, Hung 1978) giving an insight into the density, architecture, histochemical variety and relations between plexus mucosus and Lieberkühn's glands the herewith presented electron microscopic results reveal mainly neuro-cellular relations from the lamina propria. This means that the plexus mucosus has mainly the function to innervate blood vessels and lymphatics as well as smooth muscle cells and connective tissue cells (mast cells, histiocytes, plasma cells) of the small intestine mucosa.

Animals↗
PubMed1978

Procedure for the simultaneous large-scale isolation of pullulanase and 1,4-alpha-glucan phosphorylase from Klebsiella pneumoniae involving liquid-liquid separations.

A procedure for the simultaneous large-scale isolation of pullulanase and 1,4-alpha-glucan phosphorylase from Klebsiella pneumoniae is described. The pullulanase is solubilized from the cell wall by cholate treatment; cells and cell debris are removed by partition in a poly(ethylene glycol) (PEG)-dextran two-phase system and from the upper (PEG) phase of this system the pullulanase is isolated by ultrafiltration and precipitation with N-cetyl,N-,N-,N-trimethyl ammonium bromide to a purity of about 80% with a yield of 70%. The preparations are free of alpha-amylase activity. The cell containing dextran-rich phase is passed through a Manton-Gaulin homogenizer. Then the phosphorylase is separated from the cell debris by partition in a second PEG-dextran system. From the top phase of this system the phosphorylase is isolated by distribution in a PEG-salt two-phase system followed by batch adsorption on carboxymethyl-Sephadex in a yield of 55%, a purity of around 90%, and nearly free of glycosyltransferase activity. All steps in the isolation of the two enzymes can be performed easily in a large scale.

Cell Fractionation↗
PubMed1978

[Vascularization of the plexus myentericus (Auerbach) in the small intestine of swine and cats].

The ganglia of the plexus myentericus (Auerbach) have their own self-acting vascularization in the form of periganglionic capillary networks. As to the architecture and density, they are quite different from the intramuscular capillary bed. Just as the arterial trunk and arcade vessels, the terminal arterioles and sphincter capillaries running into the periganglionic cappillary network are innervated by noradrenergic axons. Together with periganglionic arteriovenous short circuits, this means favorable prerequisites for a functionally adapted blood supply of the ganglia. The specific arrangement of intramuscular vessels and the plexus Auerbach effects the maintenance of the close topographic and functional relations between both systems in all cases of changes of the shape of the intestinal wall.

Animals↗
PubMed1978

[The vascularization of the submucous external plexus (Schabadasch) and the submucous internal plexus (Meissner) in the small intestine of swine and cat].

Each ganglion of the plexus Schabadasch is supplied by a specific periganglionic capillary network. Within the plexus Meissner, several ganglia are connected to circulation areas. The communicating branches of the plexus Schabadasch have their own capillary system; as to the plexus Meissner, this is valid for the cat only. The results allow to conclude that the centres of the intramural nervous system of the intestinal wall are equipped with a preferred and self-acting vascularization.

Anatomy, Comparative↗
PubMed1978

[Light microscopic and histochemical results on the innervation of Brunner's glands in the duodenum of laboratory animals (author's transl)].

Brunner's glands of cats and guinea-pigs for instance are staffed with a well formed nerve plexus of cholinergic and adrenergic axons. Corresponding to numerous neurophysiologic results the excretory glandular cells and the enterochromaffine cells of Brunner's glands are subjected to a differentiated adreno-cholinergic influence.

Acetylcholinesterase↗
PubMed1977

[Vascularization of plexus myentericus (Auerbach) in the large intestine of the cat].

1. Coincidental preparation of the intramuscular vascular bed and the plexus myentericus (Auerbach) of the cat's large intestine by India-ink method and silverimpregnation allowed to demonstrate independent vascularisation of ganglia and nerve-branches of the plexus Auerbach. 2. Each ganglion is surrounded by a capillary network widely independently existing of the intramuscular capillary bed. The preferred innervated terminal arterioles and especially the sphincteric capillaries opening into the periganglionic capillary network and the numerous arterio-venous short-circuits in its marginal area suggest to conclude a differentiated regulation of blood supply.

Animals↗
PubMed1977

[Differentiated vascularization of the Dogiel cell types and the preferred vascularization of type I cells in the ganglia of plexus submucosus externus (Schabadasch) of the swine].

1. In analyzing the variations in form and density of the periganglionic capillary networks of the plexus Schabadasch there could unequivocally be shown a manifold better vascularisation of type I cell aggregates, compared to those of type II. 2. The preferred vascularisation of type I cells is discussed in relation to the function of the cell types of Dogiel.

Animals↗
PubMed1977

[The external submucous plexus (Schabadasch) in the small intestine of the swine. I. Form, structure and connections of ganglia and nerve cells].

1. There are described principles of organization of the plexus submucous externus (Schabadasch) in the small intestine of the pig. They can be summarized under the term of polarization of nerve cells. 2. The principle of polarization is realized for instance by the formation of type I and type II cell aggregates and by the specific course of the processes of both cell types. 3. These criteria of organization which are much clearer in the pig than in other animals, recommend the plexus Schabadasch of the pig as a suitable model for further investigations.

Animals↗
PubMed1976

[Afferent nerve endings in the small intestine and the stomach. Light and electron microscopic studies].

1. In the mucous membrane of the duodenum and pylorus there have been identified free club- and tree like endings by ligth and electron microscopy. These endings lie flat between the muscularis mucosae and the glandular basis. 2. In the duodenum of the dog the receptors are undoubtly formed by myelinated axons. 3. According to neurophysiological results these free afferent endings are similar to PAINTAL'S (1957, 1963) mucosal mechanoreceptors.

Animals↗
PubMed1976

[Experiences with the glyoxylic-acid fluorescence method for the demonstration of biogenic amines in cuticle preparations, cryostat- and simple frozen sections].

1. Our studies aimed at methodical simplification to make available the glyoxylic acid-fluorescence method to everybody. We succeeded in receiving adequate results in the case of whole mounts, cryostat- and simple frozen sections. 2. Making ready the slide preparations may be divided into 5 steps:--incubation of fresh tissue in glyoxylic acid of 1...2% (pH-value 7.0)--making ready whole mounts, cryostat-or simple frozen sections--air-drying--exposure to 80...100 degrees C for 10...3 minutes--embedding with paraffinum subliquidum. Fluorescence microscopy and photography may be done in the bright and dark field, as well with usual filter-combinations.

Animals↗