PubMed Health⌕ Search

Biomedical subjects

W Storch

Publications and source records attributed to W Storch.

At least 37 records · Page 2Linked to original sources
PubMed1984

[Possibilities for the use of immunofluorescence in clinical immunologic diagnosis].

Immunofluorescence techniques are increasingly used as a diagnostic tool in clinical immunology and pathology because of their flexibility and specificity. The main applications at present are: detection of circulating autoantibodies, detection of antigens in cells and cell membranes, detection of immune complexes in biopsy material and detection of circulating immune cells. The double immunofluorescence technique is the method of choice for the simultaneous detection of different antigens in the same localization. It has been successful used for the detection of hepatitis B surface and core antigen or for the hepatitis B surface antigen and the fourth component of complement in liver cells as well as new organ specific antibodies, e.g. against hormone producing cells, and skeletal motor endplates. The usual indirect immunofluorescence is also suitable for detecting of new antibodies, if the different staining patterns is taken into account and appropriate controls are performed (e.g. subtypes of antimitochondrial antibodies, antibodies to liver typical ribosomes, and to lamina propria of gastric mucosa.

Antigen-Antibody Complex↗
PubMed1983

[Immuno-histological findings of the liver in virus-related autoimmune hepatitis--preliminary findings].

As virus-associated autoimmune hepatitis a frequently chronic HBsAg-seronegative hepatitis was defined, in which in the liver tissue virus-markers and in the serum high titre (titre more than 1:320) autoantibodies are to be proved. The immunohistological findings of the liver of 11 patients with such a disease suggest a hepatitis B-virus, a hepatitis non-A/non-B-virus and combination forms. The solitary occurrence of virus-markers seems to be typical. In the hepatitis B-associated form furthermore a diffuse IgG-deposition at the liver cell membrane was characteristic as well as focal cytoplasmatic deposits of IgG, IgA, IgM and of C 3. In the hepatitis non-A/non-B-associated form solitarily above all nuclearly a non-A/non-B-associated-antigen, focally nuclearly a non-A/non-B-associated-antigen, focally nuclearly and cytoplasmatically IgE and diffusely cytoplasmatically IgG (with lobule-central accent) was found. The immunohistological findings, particularly the solitary occurrence of virus-markers, might be an expression of a genetically determined, no doubt, increased but incomplete immune attack of the host to the virus-infected liver cells.

Autoantibodies↗
PubMed1983

[Possibilities of immunofluorescence in clinico-immunologic diagnosis].

The immunofluorescence still represents the essential method of the proof of antibodies circulating in the serum, which are of clinical interest as diagnostic markers of autoimmune diseases. The consideration of subtypes of antibodies (instance antimitochondrial antibodies) additionally alleviates the differential diagnosis. Newer methods of immunofluorescence, such as the double-immunofluorescence for the proof of antibodies against hormone-producing cells and against motorie end-plates, further the solid phase fluorescence immunoassay and the fluoroimmunocytoadherence will in future increasingly be used. Up to now the possibility was little used to use the antibodies circulating in our patients for the diagnosis of non-immunological diseases and the cell-biological diagnosis.

Antibodies↗
PubMed1983

[Possibilities and value of immunofluorescence in the diagnosis of liver diseases].

The immunohistological investigation of liver tissue is at present regarded as method of choice for the differential diagnosis of the virus hepatitis. The quantity and distribution of various viral antigens of the hepatitis A, B and non-A/non-B furthermore allow statements concerning the prognosis. Moreover, they authoritatively influence etiopathogenetical and therapeutical concepts, also on account of the probably simultaneous proof of immunoglobulins and complement components. With the help of immunohistological methods also metabolic diseases (e.g. an alpha 1-antitrypsin deficiency) may clearly be diagnosed, when a diagnosis from the serum is not possible (among other post mortem). As an instance for scientific investigations the proof of complement in inclusion bodies of the liver cell and in so-called biliary thrombi are mentioned. Possibly in future the immunohistology is also mentioned for the early diagnosis of liver tumours.

Antibodies, Viral↗
PubMed1983

[Diagnostic significance of antibodies against cell organelles (mitochondria, endoplasmic reticulum and ribosomes)].

Sera from 5154 subjects comprising patients with liver diseases (n = 1311), immunological and connective tissue disorders (n = 1098) and other diseases (n = 2421), and healthy people (n = 324), were investigated by immunofluorescence for IgG antibodies (titre greater than or equal to 1:20) against mitochondria (AMA), endoplasmic reticulum (AER), nonorganspecific ribosomes (ARA-1), and liver typical ribosomes (ARA-2). AMA were classified in 10 subtypes. AMA (n = 163 = total 3.2%) were found predominantly in liver diseases (n = 101 = 7.7%) and in autoimmunopathies (n = 34 = 3.1%). AER (n = 59 = total 1.1%) were detected mainly in liver diseases (n = 40 = 3%), ARA-1 (n = 14 = total 3%) in autoimmunopathies (n = 7 = 0.64%), and ARA-2 (n = 7 = total 0.14%) exclusively in liver diseases (n = 7 = 0.5%) (acute and chronic relapsing hepatitis with viral markers). The diagnostic value of antibodies depends among other things on the titre and the subtype. AMA of type 2 and 4 with a titre higher than 1:1280 are a valuable marker of primary biliary cirrhosis or a related disease, even in the absence of other diagnostic signs. AER with a titre higher than 1:320 suggest a special form of autoimmune chronic hepatitis with rapid progress to liver cirrhosis. The diagnostic value of other antimitochondrial antibodies (especially those of type 5 to 10) and of antiribosomal antibodies is not exactly known.

Antibodies↗
PubMed1983

[Immunofluorescent optical detection of HBSAG in the human small intestine in chronic aggressive HBSAG-seronegative hepatitis with diarrhea].

HBsAg has been detected by direct immunofluorescence in the small bowel biopsy specimen of a 16 a old male patient with diarrhoea and HBsAg seronegative (autoimmune) chronic aggressive hepatitis. HBsAg was localized focally in apical regions of intestinal crypts, in the cytoplasm, and on their cell membranes as well as in vascular endothelium. Pretreatment of the tissue sections with unlabelled anti-IgM, anti-IgG, and fresh human serum did not block the direct staining for HBsAg. Antisera to IgG, the complement components C1q, C4, and C5 as well as to fibrinogen and FITC-Staphylococcus protein A were bound in a similar manner. Therefore, it may be reasonable to assume, that hepatitis B virus can infect the human small intestine and could be regarded as one of rare gastroenteritic viruses.

Adolescent↗
PubMed1982

Fluorescent patterns of antibodies to mitochondria, endoplasmic reticulum and ribosomes.

The fluorescent patterns of autoantibodies to mitochondria, endoplasmic reticulum and ribosomes are described. Mitochondrial autoantibodies can be now divided in 10 types, microsomal antibodies at least in 3 types. Using rat renal tissue sections the typical fluorescent pattern of new antimitochondrial antibodies were: type 7, distal tubulus and third portion of proximal tubules, type 8 distal tubules, only, type 9 first and second portion of proximal tubules, type 10 strong reaction of distal and third portion of proximal tubules. The new pattern of ribosomal antibody is characterized by a cytoplasmic staining of rat hepatocytes especially in the central area of the liver lobule. The heterogeneity of cytoplasmic autoantibodies can be used as a diagnostic marker in different pathological conditions, if the different staining patterns is taken into account.

Animals↗
PubMed1982

Storage of the complement components C4, C3, and C 3-activator in the human liver as PAS-negative globular hyaline bodies.

Liver biopsies of a 58-year-old clinically healthy patient with a hepatomegaly and intracisternal PAS-negative globular hyaline bodies were immunofluorescent-optically examined for the content of the complement components C 1 q, C 4, C 9, C 1-inactivator, C 3-activator. Further examinations were performed for fibrinogen, IgG, IgA, IgM, IgD, IgE, L-chain (type chi and lambda), alpha 1-antitrypsin, alpha 1-fetoprotein, alpha 1- and alpha 2-glycoprotein, cholinesterase, ceruloplasmin, myoglobin, hemopexin, HBsAg and HBsAg. Th inclusion bodies reacted with antisera against the complement components C 4, C 3 and C 3-activator, as also identified by double immunofluorescence. Probably this is a disturbance of the protein metabolism of the liver cell with abnormal complement storage in the presence of normal total complement and normal complement components in the serum.

Complement Activating Enzymes↗
PubMed1982

[Intranuclear alpha 1-antitrypsin in HBs-Ag-seronegative hepatitis B with PAS-negative globular hyaline bodies containing the complements components C4, C3, and C3-activator (author's transl)].

In the liver biopsy specimen of a 16 year old patient with HBsAg-seronegative hepatitis B PAS-negative globular hyaline bodies reacted with antibodies to the complement components C4, C3, and C3-activator, but not with antibodies to alpha 1-antitrypsin. However, alpha 1-antitrypsin could be identified in the liver cell nuclei, cytoplasm, and cell membrane. Probably, these findings are caused by an hereditary or acquired metabolic disorder.

Adolescent↗
PubMed1982

[Double immunofluorescence detection of HBsAg and HBcAg in Councilman bodies of the liver].

It could be shown by double immune fluorescence methods that HBsAg and/or HBcAg were present in Councilman bodies of the liver as well as in normal hepatocytes in biopsy specimens of a patient with HBsAg-seronegative chronic active hepatitis. These findings support earlier observations, that HBsAg is deposited in necrotic liver cells, and it supports the hypothesis, that Councilman bodies are formed under the influence of virus.

Fluorescent Antibody Technique↗
PubMed1982

[Serum immunoglobulins and third complement component in and after acute non-A/non-B hepatitis (author's transl)].

The serum levels of IgG, IgA, IgM, IgD and IgE and of the third complement component (C 3) were determined by the single radial immunodiffusion method in 69 serum samples of 34 female patients during (2nd and 4th week) acute non-A/non-B hepatitis and 2 years after infection. The levels were compared with those of circulating immune complexes measured by polyethylene glycol precipitation method. The levels of immunoglobulins and C 3 were similar to those of healthy persons. During the course of disease there were no significant relations with exception of an increase of IgD levels in patients with chronic course. The positive correlation of the levels of IgM and immune complexes at the first and second serum sample (r = 0,5914, r = 0,6366 respectively, p less than 0,001) could not be verified in patients with noncomplicated course (r = 0,203 8, n. s.) but it was highly significant in patients with chronic course (r = 0,9429, p less than 0,001). Determinations of immunoglobulins and immune complexes may therefore be prognostically helpful in patients with non-A/non-B hepatitis.

Adult↗
PubMed1982

[Immunohistochemical demonstration of the complement component C 4 in bile thrombi and liver cells in pseudoglandular transformation of hepatocytes (author's transl)].

The paper describes the immunohistochemical demonstration of complement component C 4 in a case of pseudoglandular transformation of liver cells caused probably by viral hepatitis. C 4 could be found first of all in the lumen of dilated bile canaliculi (in position with "bile thrombi"), further in the cytoplasm of hepatocytes and Kupffer cells, on their plasma membranes, in the sinusoids, and in necrosis. Probably, these observations indicate a disturbance of the synthesis and catabolic mechanisms of complement and/or of immune complexes. The findings so far support the hypothesis that the pseudoglandular transformation of liver cells is the vain attempt of the liver to eliminate or sequestrate of own or foreign material, e.g. immune complexes.

Adult↗
PubMed1981

[Immunohistochemical localization of actin and myosin in liver, kidney, stomach, heart and skeletal muscle: a reference to a cytoplasmic actin fibrillar network in liver cells (author's transl)].

For immunohistological localizing of actin and myosin in tissue sections, unfixed cryostate sections of rat liver, kidney, stomach, heart, and tongue as well as isolated bovine skeletal myofibrils were incubated with rabbit antibodies against actin respectively myosin from chicken gizzard and detected with anti-rabbit immunoglobulin labeled with fluorescein isothiocyanate. No remarkable differences were found between the 2 antisera. However the results suggested that a fibrillar cytoplasmic net exist especially in hepatocytes and which contains actin and may connect the cell membrane with the endoplasmic reticulum and the nucleus. The net might have static and dynamic functions.

Actins↗
PubMed1981

[Circulating autoantibodies and immune complexes in and after acute non-A/non-B hepatitis].

Serum samples from 35 female patients with and after acute non-A/non-B-hepatitis following application of human immunoglobulin "Anti-D (Rh0)" were tested for circulating antibodies against nuclei, smooth muscle, vascular endothelium connective tissue, mitochondria, ribosomes, endoplasmatic reticulum, stomach parietal cells, and other by indirect immunofluorescence and for circulating immune complexes by polyethylene glycol method. Tests were performed twice in the first weeks of illness and about 2 years after infection. 5 patients (14%) had increased levels of immune complexes, detected only in the early stage of illness. The most important early antibodies were low titre IgG-antibodies against smooth muscle antigens (68%), connective tissue (27%) and IgM-rat heart antinuclear factors (31%). 2 patients with recidival and chronic hepatitis had IgG-antimitochondrial antibodies 2 years after infection (titre I : 40 and I : 160). The results suggest, that special antibodies could be involved in the course of special form of non-A/non-B hepatitis.

Adolescent↗
PubMed1981

[The evidence of antibodies against motor endplates of the skeletal muscle in myasthenia gravis with the double immune-fluorescence technique (author's transl)].

Studies were undertaken on sera of 66 patients for detecting antibodies to motor endplates using a double immunofluorescence method according to Sondag-Tschroots et al. Rat tongue was used as antigenic substrate. Strong positive results at a serum dilution of 1: 20 were found only in patients with myasthenia gravis (15 out of 42 patients approximately equal to 35.7%). Titres between 1: 20 and 1: 1,280 occurred. 3 of 8 patients with chronic active hepatitis and with circulating antibodies to smooth muscle and 2 of 4 patients with autoimmune disorders and weakly positive antibodies in their 1: 20 diluted serum. The used rat tongue as antigenic substrate seems to be more suitable than diaphragm for detecting of such and other antibodies.

Autoantibodies↗
PubMed1981

[Heterogeneity of antimitochondrial antibodies: evidence for antigens in the mitochondrial matrix and outer membranes (author's transl)].

Human sera of patients with liver diseases (n = 7) and with systemic lupus erythematosus (n = 1) containing different antimitochondrial and other antibodies detected by immunofluorescence were reexamined after absorption with various subcellular mitochondrial fractions (whole extract, enriched inner and outer membranes, matrix, respectively). The findings were compared with those of the complement binding tests. The results suggest, that the antimitochondrial antibodies are directed against different antigens localized in the inner as well as in the outer membranes, in the matrix, and in other compartments of mitochondria, especially antibodies of type 4, 5, 7 and 8.

Adolescent↗