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Biomedical subjects

W Storch

Publications and source records attributed to W Storch.

At least 73 records · Page 4Linked to original sources
PubMed1975

[Identification of antibodies by means of immunofluorescence in autoimmune diseases].

A short survey of the methods of the immunofluorescence microscopy for the demonstration of various humoral antibodies is given. It is referred to the necessity of a far-reaching standardisation, whereby especially exact data in publications are necessary. Apart from the fluorochromium/protein ratio as conjuncate criteria among others have proved the ratio of antibody protein to the total protein content and the suitability test at the antigenic material concerned. The following antibodies are dealt with in particulars; antinuclear factors, antibodies of thrombocytes, antibodies of the skeletal muscles, antibodies against smooth musculature, antibodies against mitochondria, antibodies of parietal cells, antibodies against microsomas and antibodies against structures of connective tissue.

Antibodies↗
PubMed1975

[Demonstration of immunoglobulin determinants on lymphocyte surfaces by membrane immunofluorescence].

Isolated lymphocytes from peripheral blood of 22 healthy donors were examined with the help of the indirect membrane immunofluorescence (MIF) for immunoglobulin determinants on the surface of the cells. The quantity of MIF+-cells for IgM was on the average 8.7% (3-18) and for IgG 15.5%(8-18). Five different anti-IgM-sera were characterised with regard to their use in the MIF (precipitation titre, entire protein content, content of anti-IgM-antibodies). Differences in the quantity of the MIF+-lymphocytes were above all based on differing antibody concentrations of the sera. It was referred to further possible causes for these differences. The influence on the results of the examinations by protein addition and trypsination.

Adult↗
PubMed1975

Demonstration of membrane-bound immunoglobulin determinants and the significance of a quantitative characterization of the antisera used.

Lymphocytes from 22 healthy blood donors were tested for the presence of immunoglobulin determinants on their surfaces by indirect membrane immunofluorescence. The proportion of MemIF+ cells was, on the average, 8.7% (3-18) for IgM and 15.8% (8-28) for IgG. The small differences in the proportion of MemIF+ lymphocytes upon use of various anti-IgM sera could be attributed mainly to the different antibody concentrations. The importance of a quantitative characterization of the antisera used was pointed out.

Adult↗