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Biomedical subjects

W Turkiewicz

Publications and source records attributed to W Turkiewicz.

4 recordsLinked to original sources
PubMed2004

Distribution of the DAZ gene transcripts in human testis.

Involvement of variety of genes, especially located on Y chromosome, is critical for the regulation of spermatogenesis. In particular, fertility candidate genes such as deleted in azoospermia (DAZ) are believed to have important function in sperm production, since DAZ is frequently deleted in azoospermic and severy oligozoospermic men. The role of the DAZ gene is supported by its exclusive expression in the testis and by its deletion in about 10% of azoospermic and severely oligozoospermic patients. The distribution of DAZ transcripts in seminiferous epithelium of human testis is reported in the present study. The use of Adobe Photoshop and Scion Image softwares allowed for semi-quantitative analysis of in situ RT-PCR (ISRT-PCR) results. The intensity of ISRT-PCR product's fluorescence was different within individual seminiferous tubules. It was clearly shown by using the pseudocolour scale and transforming the intensity of the fluorescence into levels of greyscale images. The more intense fluorescence characterised single spermatogonia and those organized in small groups inside separate tubules. The most intense accumulation of DAZ mRNA was observed in spermatogonia.

Deleted in Azoospermia 1 Protein↗
PubMed1999

Vocational rehabilitation following kidney transplantation.

Successful kidney transplantation (KT) improves significantly the quality of life as compared with hemodialysis. Vocational rehabilitation and professional activity are the vital part of general rehabilitation and play an important role in determining the quality of life. The aim of this study was to evaluate the degree of vocational rehabilitation of the patients following allogenic kidney transplantation. 114 patients were questionnaired. Evaluation of the data revealed that only 31.6% of the study group returned to work despite good quality of life and satisfactory graft function of more than 75% of the KT patients. Factors influencing re-employment included gender, age and education level. Most of the patients were re-employed within 6 months following KT. In 78.9% of the cases formal disability status was not changed after KT and remained discrepant with good general condition and graft function. Results of the study allow authors to conclude that unless vocational rehabilitation of the KT patients is not improved, the chances of return to full social life given by KT are mostly wasted.

Adolescent↗
PubMed1993

Soluble E-selectin in arthritis.

Cellular adhesion molecules, such as E-selectin, function to recruit leukocytes into inflammatory lesions. Recently, a soluble form of this adhesion molecule, sE-selectin, has been described. In this study we determined soluble E-selectin (sE-selectin) in synovial fluid (SF) and blood samples from patients with rheumatoid arthritis (RA) and other inflammatory disorders and correlated sE-selectin levels with clinical parameters of disease activity. SF, blood, and cells isolated from RA SFs and synovial tissues (STs) were examined from 76 patients. In addition, normal plasma as well as supernatants from cultured human umbilical vein endothelial cells (HUVECs) were obtained. sE-selectin levels were assayed in these fluids and cell supernatants by an enzyme-linked immunoabsorbant (ELISA) assay. SFs from patients with RA had significantly higher sE-selectin levels than did those from osteoarthritis (OA) SFs (P < 0.05). SF sE-selectin levels were correlated with SF leukocyte counts. HUVECs, or RA ST cells enriched in endothelial cells, produced sE-selectin. Neutrophils isolated from RA SFs did not release sE-selectin. SF soluble intercellular adhesion molecule-1 levels correlated positively with sE-selectin levels. We conclude that sE-selectin levels are increased in SFs from RA compared to those from OA. Endothelial cells derived from umbilical vein or from RA STs release sE-selectin. Thus, sE-selectin may be important in the migration of inflammatory leukocytes into diseased RA STs and SFs.

Arthritis↗