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Biomedical subjects

W Unger

Publications and source records attributed to W Unger.

At least 19 recordsLinked to original sources
PubMed2000

Repair in the rabbit outflow system.

An in vivo study was conducted to study repair processes in the injured rabbit outflow system. A uniform injury was produced by raising intraocular pressure (IOP) manometrically to 70 mmHg for 1 h. The recovery process, which was followed clinically for 8 weeks and morphologically for 6 weeks, led to the re-establishment of normal meshwork architecture within this period. The morphological studies included light microscopy, autoradiography and electron microscopy. The initial lesion consisted of large deficits in the meshwork with breakdown of cell-to-cell connections, loss of extracellular materials and disruption of the vessels of the aqueous plexus. There was a significant lowering of IOP in the first week of recovery, which thereafter climbed back to normal. Also in the first week the meshwork became infiltrated with inflammatory cells which cleared by 4 weeks. There was some meshwork cell death by either necrosis or apoptosis. The majority of meshwork cells became activated within the first few days and remained activated for at least the first 2 weeks. Tritiated proline incorporation was maximal between 1 and 2 weeks. Tritiated thymidine labelling was seen throughout, but only after the inflammation subsided was it clear that meshwork cells in all regions of the meshwork were proliferating. Our study provided no evidence that normal meshwork cells have a basal proliferative turnover level. Our injury model involved complete repair of the outflow tissues and that required meshwork cells to become activated, mobilise, undertake synthetic activity and proliferate. This is the first example, other than argon laser trabeculoplasty, where meshwork cells in vivo have been induced to divide. Possible therapeutic implications for glaucoma are discussed.

Animals↗
PubMed2000

Systemic administration of the propargylamine CGP 3466B prevents behavioural and morphological deficits in rats with 6-hydroxydopamine-induced lesions in the substantia nigra.

The ability of CGP 3466B to attenuate the behavioural and morphological consequences of experimentally induced cell death was investigated in a recently updated animal model of Parkinson's disease. 6-Hydroxydopamine was infused bilaterally into the substantia nigra pars compacta of rats that were pretreated with desimipramine. Treatment with CGP 3466B (0.0014-1.4 mg/kg, injected subcutaneously) or its solvent was begun 2 h after the 6-OHDA injection, and maintained twice daily for 14 days. After a washout period of 14 days, changes in motor behaviour were evaluated, using the open field test (analysis of normal and abnormal stepping, e.g.) and the paw test (analysis of retraction time of limbs). Changes in learning and memory were evaluated with the help of the Morris water maze task. Following immunocytochemical staining of tyrosine hydroxylase, the extent of the lesion was quantified using a computerized system. CGP 3466B prevented all deficits produced by 6-hydroxydopamine (6-OHDA), though at different doses. It prevented: abnormal stepping (0.0014-0.014 mg/kg); increased forelimb and hindlimb retraction time (0.014-0.14 mg/kg and 0.0014-0.14 mg/kg, respectively); delayed learning (1.4 mg/kg); and reduced tyrosine hydroxylase immunoreactivity in the substantia nigra (0.0014-0.014 mg/kg). CGP 3466B (0.0014-0.14 mg/kg) induced no deficits in sham-treated rats. CGP 3466B (1.4 mg/kg), however, did not show any benefit on motor deficits in 6-OHDA-lesioned rats, and induced abnormal movements and decreased the tyrosine hydroxylase immunoreactivity in the substantia nigra pars compacta and the ventral tegmental area of sham-lesioned animals. It is concluded that CGP 3466B prevents all 6-OHDA-induced behavioural and immunocytochemical deficits, though at different doses. CGP 3466B is suggested to be a valuable agent for inhibiting the dopaminergic degeneration in patients with Parkinson's disease.

Animals↗
PubMed1994

The effect of finasteride, a 5 alpha-reductase inhibitor, on scalp skin testosterone and dihydrotestosterone concentrations in patients with male pattern baldness.

The effects of the 5 alpha-reductase inhibitor, finasteride, on scalp skin testosterone (T) and dihydrotestosterone (DHT) levels were studied in patients with male pattern baldness. In a double blind study, male patients undergoing hair transplantation were treated with oral finasteride (5 mg/day) or placebo for 28 days. Scalp skin biopsies were obtained before and after treatment for measurement of T and DHT by high pressure liquid chromatography-RIA. In 10 male subjects studied at baseline, mean (+/- SEM) DHT levels were significantly higher in bald (7.37 +/- 1.24 pmol/g) compared to hair-containing (4.20 +/- 0.65 pmol/g) scalp, whereas there was no difference in mean T levels at baseline. In bald scalp from 8 patients treated with finasteride, the mean DHT concentration decreased from 6.40 +/- 1.07 pmol/g at baseline to 3.62 +/- 0.38 pmol/g on day 28. Scalp T levels increased in 6 of 8 subjects treated with finasteride. Finasteride decreased the mean serum DHT concentration from 1.36 +/- 0.18 nmol/L (n = 8) at baseline to 0.46 +/- 0.10 nmol/L on day 28 and had no effect on serum T. There were no significant changes in scalp or serum T or DHT in placebo-treated patients. In this study, male subjects treated with 5 mg/day finasteride for 4 weeks had significantly decreased concentrations of DHT in bald scalp, resulting in a mean level similar to the baseline levels found in hair-containing scalp.

5-alpha Reductase Inhibitors↗
PubMed1991

A review of the racial differences in the lung function of normal Caucasian, Chinese and Indian subjects.

In the clinic, predicted normal values are widely used for routine assessments and, therefore, it is important that the noted differences in lung volumes between the races are recognized. Further epidemiological studies, using comparative methods and standard statistical techniques, are essential in order to quantify apparent differences between the races and to standardize normal values for race, especially with regard to age, with greater accuracy.

Adult↗
PubMed1990

[Results of sonographically-guided percutaneous procedures as therapy of liquid-filled space-occupying lesions in acute abdomen].

In the period from 1985 emergency sonographic study has been performed in 56 patients. In 44 cases we found intraabdominal abscesses, in 5 liver cysts with internal bleeding, in 5 postoperative lymphatic cysts, and in 2 cases loculated pleural empyemas. In 35 patients ultrasound guided therapeutic procedures were performed; there were 13 punctures (some repeated) and 22 drainages. In 29 patients no further surgical treatment was necessary. The over all success rate was 82.9%. One patient developed bacteremia after percutaneous drainage and an other developed subcutaneous abscess (complication rate: 5.7%). When abscesses are sufficiently drained no antibiotic therapy is needed.

Abdomen, Acute↗
PubMed1990

Regional distribution of lysosomal enzymes in the canine retinal pigment epithelium.

We have investigated the activities of four lysosomal enzymes in RPE cells isolated from three regions of the canine fundus: the tapetal area, the central pigmented area and the peripheral area. The results obtained with freshly isolated cells showed that the activities of acid phosphatase, B-glucuronidase and N-acetyl-B-glucosaminidase were significantly higher in RPE cells derived from the peripheral region when compared to those from the two central regions. In contrast, the activity of cathepsin D was significantly higher in the tapetal region than in the periphery. The regional distribution of both acid phosphatase and B-glucuronidase observed in fresh RPE cells was progressively lost when these cells were grown in culture. Estimations of photoreceptor density per RPE cell from each of the regions indicated that the number of photoreceptors per RPE cell did not vary significantly with retinal location and suggested that variations in enzyme content were not related to differences in photoreceptor cell distribution.

Animals↗
PubMed1989

[Surgical treatment of hemorrhaging gastroduodenal ulcer. Individually adapted indications].

Increase in application of emergency gastroscopy together with indications according to "Forrest" criteria, in the course of nine years, resulted in reduction of lethality by 15 per cent among patients who had been surgically treated for bleeding ulcers. Life-threatening postoperative risks were higher without gastroscopy. A critical appraisal is made in this paper of gastroscopic findings, with reference being made to surgical results.

Aged↗
PubMed1988

Inflammation and the formation of epiretinal membranes.

In this review of the literature evidence is provided from clinical, histological and experimental sources that inflammatory processes play a central role in the pathogenesis of contractile epiretinal membranes and proliferative vitreoretinopathy.

Animals↗
PubMed1988

A microsystem to assay lysosomal enzyme activities in cultured retinal pigment epithelial cells.

A microsystem to assay the activity of lysosomal enzymes in a small number of cultured RPE cells is described. The activities of acid phosphatase, a-mannosidase, B-glucuronidase and N-acetyl-B-glucosaminidase were estimated in different human RPE cultures of varying passages. Some biochemical characteristics for each of the enzyme assays were studied including the effect of pH, the saturating concentrations of the appropriate substrates and the relationship between the enzyme activity and the number of cells assayed. The method presented is straightforward, avoids complicated tissue fractionation procedures and is able to estimate enzyme activities in as few as 10(4) cells.

Cells, Cultured↗
PubMed1988

Evidence for the presence of serotonergic nerves and receptors in the iris-ciliary body complex of the rabbit.

The rabbit iris-ciliary body contains 78 +/- 6 ng/gm serotonin (5-HT) while the amine content in the aqueous humor is less than 0.01 ng/100 microliter. The low levels of endogenous 5-HT in the iris-ciliary body could not be directly detected by immunocytochemistry. However, pretreatment in vivo and in vitro with L-tryptophan and pargyline resulted in the localization of a sparse population of 5-HT fibers. These fibers could not be studied by exposing the tissue to exogenous 5-HT since the amine was taken up by noradrenergic fibers as well. This was confirmed in studies involving superior cervical ganglionectomy. It is concluded that 5-HT is taken up by both serotonergic and adrenergic fibers of the iris-ciliary body. In the presence of lithium, 5-HT stimulated an increase in the 3H-inositol phosphate accumulation in a dose-dependent manner in tissue where the phosphoinositide pool was labeled with 3H-inositol. A variety of agonists and antagonists were used to show that the 5-HT response is mediated, at least partly, by 5-HT2 receptors. The 5-HT-mediated effect on inositol phosphates is unaffected by superior cervical ganglionectomy. The effect of noradrenaline, which also stimulates inositol phosphate accumulation (but via alpha 1-adrenergic receptors in the iris-ciliary body), was elevated following superior cervical ganglionectomy.

Animals↗
PubMed1987

[Gallstone ileus].

Aetiopathogenesis, diagnosis as well as typical and atypical courses are described and discussed with regard to 50 patients with gallstone ileus. Overall lethality amounted to 36 per cent, including 15 per cent for patients with enterotomy alone and 53 per cent for patients who had undergone enterotomy with faecal aspiration. Postoperative complications were recorded from 78 per cent of the survivors.

Aged↗
PubMed1985

The cells of the bovine outflow system in tissue culture.

Meshwork cells from the bovine outflow system were grown in culture and their growth characteristics and behaviour in vitro reported. The cells were studied by inverted phase microscopy and time-lapse cinephotomicrography. It was found that plating efficiency was highest for third and seventh passage cells. These cells reached confluence within 2 weeks when plated out at 7.5 X 10(3) cells cm-2 in a conventional culture medium containing 10% serum. Third passage cells were studied in greatest detail. These cells exhibited postconfluence contact inhibition of division. By 2 weeks postconfluence the division rate decreased 10-fold to a level of less than six divisions per thousand cells per day. The contact inhibition was not overcome by increasing the serum content of the medium. On the other hand, a 30-fold increased division rate could be produced after scratching the culture flask. Postconfluent contact-inhibited cultures were considered to be a better reflection of the behaviour and activities of meshwork cells in vivo than cultures which retain a high level of division.

Animals↗
PubMed1985

Daily glucose and insulin rhythms in diabetic dogs on the artificial beta cell.

The circadian periods of plasma glucose, insulin and alpha-amino nitrogen (alpha-AN) were studied in fed and fasting normal and diabetic dogs which were fed either beef or beef supplemented with carbohydrates (CH). The diabetics were either withdrawn from insulin supply or treated with an artificial beta cell (ABC) or infused a constant insulin dose (CI).--There was a significant daily glucose rhythm in normal fasting animals and in fasting diabetics on CI or on insulin withdrawal. In the fed controls, the phase of the rhythms depends on carbohydrate content of food. In CH-free fed controls the insulin maxima were related to alpha-AN but in CH-fed controls they were related to glycemia. Due to the mechanism of ABC-provided insulin dosage, the phases of glucose and of insulin oscillations were correlated in all diabetics on ABC. Thus even if the mean glucose level is normalized by ABC the intrinsic phase relations remain altered.--It is concluded that the daily glucose periodicity is based on endogenous rhythms in glucose production and utilization and is essentially independent of current insulin provision. But it is governed by the meals as main "Zeitgebers" and modified by the actual insulin supply.

Amines↗
PubMed1985

[Experiences with Lezius nailing. Report on 1,090 pertrochanteric femoral fractures].

Between 1968 and 1983, 1090 patients suffering from pertrochanteric fractures of the femur were admitted to our clinic. Internal fixation by means of the Lezius-nail was performed in 644 cases. This method has some striking advantages: load stability immediately after operation, hardly measurable loss of blood, short duration of the operative procedure and a low rate of complications (6.5%). The mortality rate amounted to 17.5%, caused by the high frequency of multimorbidity within the patient's group (average age: 80.5 years).

Aged↗
PubMed1983

Adenylate kinase activity of cerebrospinal fluid in central nervous system disorders.

Adenylate kinase activity was measured in 41 samples of cerebrospinal fluid in 34 patients with various neurological disorders or psychiatric symptomatologies. Activities of the enzyme showed to be linked to clinically estimated acuteness or progression of the changes in the central nervous system at the time of specimen collection. The findings suggest the conclusion that determination of adenylate kinase activity in cerebrospinal fluid is a meaningful tool for the evaluation of progression and/or acuteness of central nervous system disorders.

Adenylate Kinase↗
PubMed1982

[Variant creatine kinase isoenzymes: frequency and clinical significance (author's transl)].

To estimate the incidence of variant creatine kinase (CK)isoenzymes, 1,191 unselected patients were screened by means of CK:CK - MB ratio. When CK - MB was higher than 20% of the total CK in the immunoinhibition test, and a myocardial infarction could be excluded, a CK-isoenzyme electrophoresis was performed. We found macro-CK-BB-emia in 13 patients, but only three of them had elevated total CK activity. All patients but two were at the typical age of over 60 years; two males were 2 and 9 years old. The clinical diagnosis in macro-CK-BB-emia was predominantly cardiocerebrovascular insufficiency due to arteriosclerosis. In 16 other patients, the presence of an isoenzyme migrating cathodically to CK-MM was found. In this group, a malignant metastatic tumor was found in eight cases, the rest predominantly suffered from severe liver disease. Presence of both variant CK isoenzymes may lead to diagnostic or therapeutic errors due to the altered CK:CK-MB ratio. According to our studies, a variant CK isoenzyme occurs in 2.6% of hospitalized patients, but this will lead to problems diagnosing acute myocardial infarction only in the group with elevated total CK (0.42% of our patients).

Aged↗