PubMed HealthSearch

Biomedical subjects

W W Edmunds

Publications and source records attributed to W W Edmunds.

2 recordsLinked to original sources

Pilot judgment: an operational viewpoint.

The process of decision making is poorly understood in our aviation environment. Accidents may demonstrate instances where incorrect decisions were made, but current investigative techniques do not necessarily give a clear understanding of why such decisions were made. The elements of the decision-making process need to be better understood so proper attention may be given to pilot training and developing operational procedures . A study of the Aviation Safety Reporting System data base was conducted to gain a better understanding of the actual operational impact of decision making.

Accidents, Aviation

Mass transfer effects in microencapsulated hybridoma cells producing monoclonal antibodies.

Rat-mouse and mouse-mouse hybridoma cell lines were used for formation of monoclonal antibodies (MAbs) in microcapsules of different sizes. Microcapsules were made of poly L-lysine-alginate hydrogel membranes. The effects of extracapsule liquid film, intracapsule and transmembrane transfer limitations of nutrients/products on system's performance were investigated. An agitation speed of 45 rpm (4 cm/s tip speed) was found to be optimal in spinner flasks to overcome liquid film resistances around capsules. Capsule sizes need to be reduced to smaller than 350 mu in order to eliminate intracapsule transfer limitations with a typical initial viable cell concentration of 0.5 x 10(5) viable cells/mL capsule. Double coating of capsules to improve strength of capsules resulted in higher transmembrane transfer resistances.

Animals