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W W FORREST

Publications and source records attributed to W W FORREST.

9 recordsLinked to original sources
PubMed1965

SYNTHESIS OF RESERVE MATERIALS FOR ENDOGENOUS METABOLISM IN STREPTOCOCCUS FAECALIS.

Forrest, W. W. (University of Adelaide, Adelaide, South Australia), and D. J. Walker. Synthesis of reserve materials for endogenous metabolism in Streptococcus faecalis. J. Bacteriol. 89:1448-1452. 1965.-The growth curve of Streptococcus faecalis in batch culture with limited energy source shows an initial portion of exponential growth where the growth yield coefficient Y(glucose) is 32, followed by linear growth where Y(glucose) is 21. Endogenous metabolism is correlated with maintenance of glycolytic activity, and there is a marked change in the glycolytic activity of cells harvested under these conditions; however, endogenous metabolism cannot be detected in either case by measurement of the adenosine triphosphate (ATP) pool in the organisms. Cells harvested after growth with excess energy source exhibit endogenous metabolism, which is correlated with a much higher concentration of ATP in the organisms than occurs in the cells grown with limited energy source.

Adenosine Triphosphate↗
PubMed1964

ANAEROBIC ENDOGENOUS METABOLISM IN STREPTOCOCCUS FAECALIS.

Walker, D. J. (CSIRO, University of Adelaide, Adelaide, South Australia), and W. W. Forrest. Endogenous metabolism in Streptococcus faecalis. J. Bacteriol. 87:256-262. 1964.-Washed suspensions of Streptococcus faecalis incubated in phosphate buffer (pH 6.0) under anaerobic conditions released amino acids into the suspending medium. Little or no carbohydrate was released in soluble form, and no acid production could be detected. Cell lipid was stable, and only a small amount of nucleotide (material absorbing at 260 mmu) was released. Estimates of total carbon produced in soluble form showed that this could be almost entirely accounted for as amino acid. Over the short period during which amino acid was produced (up to 5 hr), the glycolytic activity of the cells remained fairly constant, and fell slowly thereafter to low values in 24 to 48 hr; the rate of fall depended on cell concentration. Whereas exogenous sources of energy protected against loss of glycolytic activity, arsenate, hydroxylamine, or replacement of phosphate by other buffers accelerated loss of glycolytic activity. Restoration of glycolytic activity in aged cells could be achieved by incubation with amino acids plus an energy source, with concurrent synthesis of protein but no apparent growth. Ammonium ion was not effective in protecting or restoring glycolytic activity. The nature of changes which took place during endogenous metabolism are discussed.

Acetates↗
PubMed1961

Enthalpy changes associated with the lactic fermentation of glucose.

Forrest, W. W. (University of Adelaide, Adelaide, South Australia), D. J. Walker, and M. F. Hopgood. Enthalpy changes associated with the lactic fermentation of glucose. J. Bacteriol. 82:685-690. 1961.-The heat produced during the fermentation of glucose by growing cultures and by washed cells of Streptococcus faecalis has been measured by direct calorimetry. The heat production of growing cultures gives a direct measure of the rate and amount of growth which has occurred. The enthalpy change when glucose is fermented essentially to lactic acid at 37 C is -29.1 cal per mmole of glucose, which is in agreement with the calculated value for the mixture of products formed.

Carbohydrate Metabolism↗