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Biomedical subjects

W W Fish

Publications and source records attributed to W W Fish.

53 records · Page 3Linked to original sources
PubMed1976

Comparison of the physical properties of chemically prepared and tissue-endogenous equine apoferritins.

To support the case for a biological applicability of previous experiments performed on chemically prepared apoferritin, its hydrodynamic and circular dichroic properties were compared to those of tissue-endogenous apoferritin. The molecular weights and sedimentation coefficients of the two differently prepared apoferritins were identical. Similarly, their circular dichroic spectra between 310 and 200 nm were identical. These data support the hypothesis that chemically prepared apoferritin is identical with tissue-endogenous apoferritin. Ferritins exhibited low ultraviolet CD spectra quite similar to apoferritin except amplitudes were often as much as 8% less. Apoferritin subunits, prepared by acid dissociation, did not possess the positive 292 and 286 nm bands exhibited by the native molecule, although hydrodynamically the subunit behaves as a globular protein. This suggests the presence of tryptophanyl and tyrosyl residues at the subunit contact sites. Two characteristics of the apoferritin CD spectrum were proposed as means to evaluate the quality of apoferritin preparations. These are a ratio, [theta]286 [theta]222 = -4.5 X 10(-3) +/- 0.2 X 10(-3), and the wavelength region, 258 to 262 nm, at which the near ultraviolet CD spectrum goes from a positive to a negative sense.

Animals↗
PubMed1975

Preliminary studies in measuring range of motion in normal and painful stiff shoulders.

This study has been prompted by the need to provide basic data for prospective studies in the management of the painful stiff shoulder. We have used a hydrogoniometric technique of proven accuracy to measure passive movement at the gleno-humeral joint in a normal population, patients currently attending with a painful stiff shoulder, and a retrospective group. We have shown that there are significant age and sex differences in the range of motion of normal and affected groups. A significant number (42 per cent) of patients had persisting limitation of range of the gleno-humeral joint six years after the onset of the disability. The study indicates the importance of matching patients for age and sex, and the need for long-term follow-up for meaningful results.

Adult↗
PubMed1975

A radiological assessment of osteoarthrosis of the knee: experiments in observer error.

In a trial to determine the best palliative physiotherapy for the painful osteoarthrosic knee, radiological assessment was suggested as a measure for testing homogeneity of treatment groups. Preliminary observer error experiments showed that an accepted standard grading scheme was unsatisfactory. A different system was designed in which all three knee compartments were graded separately (medial, lateral and patello femoral), based on a comparison of supine and erect films. The two systems have been compared in use on the same set of radiographs, and the observer error results are presented. The advantages of grading each compartment separately are discussed.

Humans↗