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W Weddell

Publications and source records attributed to W Weddell.

9 recordsLinked to original sources

A practical method for the production of Brucella skin test antigen.

Skin test antigens for the diagnosis of brucellosis were produced from the rough Brucella abortus strain 45/20. The production of two products termed Brucellin B and Brucellin W are described. The method described for the production of Brucellin W is recommended as an improved practical method of Brucellin production. The Brucellin products described were equal in sensitivity to that of a commercially available product, Brucellergen, which is used in New Zealand. Brucellin B was extensively tested in non-infected cattle and its specificity was equal to that of Brucellergen. Recommendations for the standardisation of skin test reagents for the diagnosis of brucellosis are made. Intradermal testing for brucellosis in cattle should be used only for the identification of infected herds and not as an individual animal test.

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A comparison of three serological tests for the diagnosis of B. ovis infection in rams.

The complement fixation test (CFT), the enzyme labelled immunosorbent assay (ELISA) and the gel diffusion precipitin test (GD) were compared, for the diagnosis of Brucella ovis infection in rams. The sensitivities of the tests in 109 rams which were shedding B. ovis in their semen were: CFT 96.3%; ELISA 97.2%; GD 91.7%. The specificities of the tests in 141 rams from non-infected flocks were: CFI 99.3%; ELISA 98.6%; GD 100%. Predictive values of the three tests were measured in 285 rams from infected flocks. Thirty-eight percent of these rams were shedding B. ovis in their semen. Predictive values of positive tests were: CFT 75.5%; ELISA 66.7%; GD 72.5%. Predictive values of negative tests were: CFI 97.1%; ELISA 97.6%; GD 93.8%.

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Bovine leukaemia virus infection in New Zealand cattle.

Bovine Leukaemia Virus (BLV) infection in New Zealand cattle was investigated. In a national survey of 5000 sera from 500 herds, BLV antibody was not detected. An additional 1062 sera from 140 herds were tested and 3 sera were positive. In the herd of origin of one of these 3 sera, 22.6% of cattle were serologically positive for BLV. Where cases of bovine lymphosarcoma had been diagnosed, 38 of 39 herds tested were negative for BLV antibody. Within the remaining herd, 36% of cows tested were serologically-positive. BLV was isolated from 2 serologically positive cows in this herd.

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