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W Weise

Publications and source records attributed to W Weise.

At least 19 recordsLinked to original sources
PubMed1995

Bacterial contamination of single-donor blood components.

An analysis was performed of the microbiological laboratory quality control data for the past 9 years (1985-1993). Bacterial contamination was detected in 100 of 25,171 tested blood components. Single-donor platelet concentrates had a contamination incidence of 25 in 5889 (0.42%); whole blood samples were contaminated at a rate of 1 in 2973 (0.03%) and red cell concentrates at a rate of 73 in 15,317 (0.48%); of 992 samples of fresh frozen plasma only 1 was contaminated (0.1%). Gram-positive staphylococci comprised 75% of cases and Gram-negative rods 10%. This frequency of laboratory-detected bacterial contamination contrasts with the low rate of transfusion-associated septicaemic events.

Bacteremia↗
PubMed1995

[Proteins in uterine secretions of fertile and sterile patients using SDS polyacrylamide gel electrophoresis (SDS-PAGE)].

Aim of the presented study was to find out whether there are any differences in the protein patterns of uterine secretions of fertile and sterile women. Analysis was made by polyacrylamide gel electrophoresis (SDS-PAGE). Uterine secretions and sera of 11 sterile and 8 fertile patients were investigated. Utilizing the reducing SDS-electrophoresis, the comparison of all uterine secretions revealed an additional protein band of molecular weight of 33,000 Dalton in the uterine secretions of the fertile women. Secretions of the sterile women showed a protein band which was of significantly lower weight. The significance of this additional protein band as a possible factor of fertility is discussed.

Adult↗
PubMed1995

[Lymphohistiocytic infiltration of endometrial adenocarcinoma].

Mononuclear cells adjacent to endometrial adenocarcinoma were evaluated in 8 biopsies by the immunoperoxidase technique. Between benign and malignant tissue a remarkable infiltration of CD45+, CD3+, CD4+, CD8+, and CD14+ cells could be observed. B cells (CD19) and NK cells (CD57) were rare. Tumor-infiltrating lymphocytes were only sparsely distributed. The findings suggest a cellular, yet ineffective, immune reaction of the host against the tumor at the borderline between benign and malignant tissue.

Adenocarcinoma↗
PubMed1994

Prevalence of human T-cell lymphotropic virus infections in Germany.

The extent of human T-cell lymphotropic retrovirus HTLV-I and HTLV-II infections in the general population in central Europe has not been investigated fully. Two hundred forty-eight thousand blood donors from southern Germany were examined serologically for antibodies to the human lymphotropic retroviruses HTLV-I and HTLV-II: 0.021% were confirmed positive and 0.056% were "indeterminate". A limited number of seropositives and "indeterminate" samples were analyzed by polymerase chain reaction (PCR): the seropositives were confirmed as positive and 43% of the "indeterminate" samples were PCR-positive. The range of 0.021% HTLV-positives in 248,000 donors, i.e. about two in 10,000 individuals, mirrors closely the published data for the United States.

Deltaretrovirus Antibodies↗
PubMed1994

[Effect of granulocyte colony stimulating factor (G-CSF) on peripheral blood leukocytes and lymphocytes in patients with chemotherapy-induced leukopenia].

In patients with extensive chemotherapy, G-CSF abrogated leukopenia following administration of cytotoxic agents. Six women with ovarian cancer and chemotherapy-induced leukopenia received 300 micrograms Filgrastrim (r-metHuG-CSF, Neupogen 30; AMGEN, Germany) daily for 10 days. Leukocytes and lymphocyte subsets of peripheral blood were determined before, throughout and after subcutaneous injections of G-CSF by flow cytometry using monoclonal antibodies to CD3, CD4, CD8, CD14, CD16/56, CD19 and CD45. It could be observed that not only neutrophils (23 fold) but also lymphocytes (6 fold) and monocytes (10 fold) showed a dramatic increase in cell counts throughout and after G-CSF administration. This is in contrast to previous reports, where only effects on neutrophils were described. In spite of the increase in lymphocytes the relative percentage of CD3+, CD19+, CD3-CD16/CD56+, CD3+, CD8+ and CD3+ CD4+ lymphocyte subsets did not change throughout and after therapy, except for an increased expression of HLA-DR on CD3+ lymphocytes.

Antineoplastic Combined Chemotherapy Protocols↗
PubMed1994

[Leukocyte and lymphocyte populations in peripheral blood and malignant ascites in patients with ovarian carcinoma].

Ascites and peripheral blood of 12 patients with advanced ovarian cancer (stage IV) have been investigated by two-color flow cytometry for leukocytes and lymphocyte subsets with monoclonal antibodies, against CD3, CD4, CD8, CD14, CD16/56, CD19, CD25, CD45, CD57, and HLA-DR. Ascites compared with blood showed a significant raise of CD3-positive lymphocytes (80 +/- 14% vs. 69 +/- 8%) and a significant reduction of CD57-positive lymphocytes (13.6 +/- 13% vs 24 +/- 21%). There was an increased expression of HLA-DR on CD3-positive lymphocytes in malignant ascites. The results are discussed with regard to a supposedly defective local immune defense against the tumor.

Antibodies, Monoclonal↗
PubMed1994

[Dose intensified carboplatin monotherapy in advanced ovarian cancer].

Platinum derivatives are known as the most effective cytostatic agents in ovarian carcinoma. The steep dose-response curves described by Hryniuk and Levin [5] make them the ideal substances for dose escalation. We treated patients with advanced ovarian carcinoma with dose intensified carboplatinmonotherapy six times at AUC 5 (= +/- 400 mg/m2 in patients with normal renal function). Dose intensification was not done by increasing single doses, but by shortening the intervals between therapies (23, 21, 17, 14 days). In order to prevent leucopenia G-CSF was administered at a dose of 5 micrograms/kg s.c. Besides mild emesis no extramedullary toxicity especially no alopezia was documented. Up to an interval of 17 days therapy was safe; no thrombocytopenia < 49,000 x 10(6)/L and no leucopenia < 1000 x 10(6) was observed. With the intention to arrive at a 14-day interval and to attain further dose escalation we will treat future patients with a slightly reduced dose of carboplatin (AUC 4) in combination with escalating doses of cis-platinum, which is known for its low myelotoxicity.

Adult↗
PubMed1994

[HIV retrospective study of the German Red Cross blood donation service in Germany].

OBJECTIVE: It was tried to retrospectively identify HIV infections in recipients of transfusions from donors who were tested HIV positive at a subsequent donation. These lookback data were traced back to answer the following questions: 1. How many transfusion recipients were infected before the start of the routine HIV testing in 1985? 2. How great is the risk of HIV infections from infected but not yet HIV antibody-positive donors? 3. Furthermore, the transfusion of HIV-infected transfusion recipients was traced back to the involved donor to establish causality. DESIGN: Retrospective ('lookback') study. SETTING: HIV Study Group of the Red Cross Blood Banks of the Federal Republic of Germany. PARTICIPANTS: Preceding donations of HIV antibody-positive repeat donors were traced back to the transfusion recipients in order to establish their HIV antibody status. In a second lookback study, HIV-infected transfusion recipients and their corresponding donors were investigated after they had been reported to the blood bank as infected by transfusion-associated HIV. INTERVENTIONS: None. RESULTS: Recipients of 156 respectively 133 transfusions from repeat donors found to be Western blot-positive were investigated from 1985 to 1987 and from 1987 to 1992, respectively. About 50% of the recipients had died. About 40% of the recipients could not be examined, because they either were not available for testing or refused to be tested or because it was impossible to clarify the fate of the blood products. 25 HIV recipients were identified from 1981 to 1985, when routine HIV testing began. Nine transfusion-associated HIV infections were identified from 1985 to 1992. 25 million units of blood were prepared during this period. CONCLUSIONS: The risk of HIV transmission by tested transfusions is extremely rare (in the order of 1:1 million). The second lookback study suggests that in more than 50% of the blood recipients in whom HIV infection was attributed to transfusion, a causal relationship to an infected donor could not be established.

AIDS Serodiagnosis↗
PubMed1992

Abbott HCV EIA 2nd generation: a new screening assay.

An enzyme immunoassay for the detection of antibodies against structural and nonstructural domains of the hepatitis C virus (EIA-2) was compared to a first generation test (EIA-1) that can only detect antibodies against one nonstructural antigen (c100). EIA-2 revealed elevated detection rates as assessed in high risk and patient populations as well as lower repeat reactive rates in volunteer blood donors. Research test systems proved an increase in apparent specificity.

Blood Donors↗
PubMed1992

[Experiences in dealing with tubal sterilization].

The political changes in the GDR and the unity of Germany give the women the possibility of deciding for tubal sterilization as a method of contraception that was restrictively used in the GDR. This investigation analysis the psychosocial and medical factors influencing the women in their decision for sterilization. 103 women were interviewed with a questionnaire before they underwent tubal sterilization in a great medical center in Sachsen-Anhalt. The findings known in the literature regarding the satisfied after sterilization were discussed. However the sterilization is an intervention that should be thought over carefully.

Abortion, Induced↗
PubMed1991

The examination of the ITI system in disputed paternities.

106 paternity cases with a total of 114 putative fathers were examined in the inter-alpha-trypsin inhibitor (ITI) system. Analysis was performed by isoelectric focusing (IEF) of untreated sera on polyacrylamide gels. From 39 paternity exclusions, determined in other genetic systems, 7 were confirmed in the ITI system. In 75 expertises the alleged man was not excluded from fatherhood; in 68 cases the probability of paternity was W greater than 99.73%. The practical exclusion rate in the ITI system was therefore calculated to be 10.45%. The theoretical exclusion rate was determined to be 19.3%. In one paternity case the alleged father and the child showed inverse homozygosity in the ITI system, while the man was not excluded from fatherhood in 28 additional marker systems. The calculated probability of his paternity was 99.99%. The assumption of an incomplete expression of the ITI phenotypes in infants is supported by a significant deviation between the observed and expected ITI distributions at population equilibrium.

Alpha-Globulins↗
PubMed1991

[HTLV-I antibody screening of blood donors].

Routine screening of 344,266 blood donations of approximately 200,000 donors for anti-HTLV-I was done by enzyme immuno assay (EIA). Repeatedly reactive samples were confirmed by Western Blot (WB) and radio immuno precipitation assay (RIPA). The prevalence of anti-HTLV-I in Bavarian blood donors is 0.02-0.026%. The specificity of HTLV-I-EIA is 99.95%.

Blood Donors↗
PubMed1990

[The neuraminic acid/protein quotient in amniotic fluid: indicator for fetal maturity].

The concentration of neuraminic acid and proteins was determined in amniotic fluid of 207 pregnancies during the amniocentesis, performed in the 16.-21. week of gestation, 17 prematurities and 101 deliveries in time. The neuraminic acid/protein quotient increases significantly at term. Than, its reference range is 51.3-154.8 mumol/g (2.5-97.5 percentile). The neuraminic acid/protein quotient can be an useful prenatal indicator of the fetal maturity because of its high diagnostic specificity (97%) and sensitivity (88%).

Amniocentesis↗
PubMed1988

[Quantitative detection of spermatogenesis cells and qualitative assessment of meiosis stages in the ejaculate of normo- and oligozoospermia].

Meiosis examinations in semen have been aspired in 106 patients of an andrologic outpatient clinic. Diakinesis and metaphase could be represented in 15 per cent. No meiosis analysis was possible in 12 per cent of the males. The increase of the immature spermatozoa count from 8 per cent in case of normozoospermia to 55 per cent in oligozoospermia III. degree was highly significant. Better quality of meiosis representation correlated to the increasing number of spermatogenesis cells.

Adult↗