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Biomedical subjects

W X Zhao

Publications and source records attributed to W X Zhao.

At least 19 recordsLinked to original sources
PubMed2006

Protective effects of propofol on lipopolysaccharide-activated endothelial cell barrier dysfunction.

BACKGROUND: Propofol has been widely used in intravenous anesthesia. It possesses antioxidant and immunomodulating effects. This study aimed to investigate whether propofol may attenuate lipopolysaccharide (LPS)-induced endothelial cell barrier dysfunction and the possible mechanisms of such modulation. METHODS: Cultured human umbilical vein endothelial cells (HUVECs) were used to assess the following treatments: (i) no additives (negative control), (ii) LPS alone (1 and 10 microg/ml), (iii) propofol alone (20 microg/ml), (iv) intralipid (a solvent of propofol) alone (20 microg/ml), (v) LPS (10 microg/ml) combination with propofol (4 and 20 microg/ml) and (vi) LPS (10 microg/ml) combination with intralipid (20 microg/ml). Changes of cell permeability and filamentous actin (F-actin) were determined. Expression of nitrotyrosine proteins and activity of nuclear factor kappaB (NF-kappaB) were analyzed by Western blot and immunocytochemistry. Expression of endothelial nitric-oxide synthase (eNOS) and inducible nitric-oxide synthase (iNOS) were analyzed by reverse transcriptase-polymerase chain reaction. RESULTS: LPS markedly increased the permeability of endothelial cells, the formation of peroxynitrite and depolymerization of F-actin in HUVECs. LPS also significantly increased mRNA of iNOS, protein level of NF-kappaB and decreased mRNA of eNOS (P < 0.05). Propofol at both concentrations (4 and 20 microg/ml) significantly inhibited the LPS-induced increase in cell permeability and alteration in F-actin organization. Propofol also reduced the LPS-enhanced iNOS mRNA and NF-kappaB protein levels whilst it increased eNOS mRNA expression (P < 0.05). CONCLUSION: This study demonstrated that propofol, both at therapeutic concentrations and 5 times therapeutic concentrations, inhibited NF-kappaB activation in LPS-stimulated endothelial cells and was found to protect endothelial cells against LPS-induced barrier dysfunction.

Actins↗
PubMed2001

Measurement of the charged pion electromagnetic form factor.

Separated longitudinal and transverse structure functions for the reaction 1H(e,e(')pi(+))n were measured in the momentum transfer region Q2 = 0.6--1.6 (GeV/c)(2) at a value of the invariant mass W = 1.95 GeV. New values for the pion charge form factor were extracted from the longitudinal cross section by using a recently developed Regge model. The results indicate that the pion form factor in this region is larger than previously assumed and is consistent with a monopole parametrization fitted to very low Q2 elastic data.

Journal Article↗
PubMed2001

[Effect of huangqi zengmian powder on interstitial response in patients with esophageal cancer at peri-operational period].

OBJECTIVE: To observe the effect of Huangqi Zengmian Powder (HQZMP) on interstitial response of esophageal cancer at peri-operational period. METHODS: Thirty-seven patients of esophageal cancer were treated with HQZMP for 4 weeks peri-operationally. The pathological interstitial changes (including mastocyte, microvessels and lymphocytic response) in resected esophageal cancer sample were observed using toluidine blue stain. RESULTS: As compared with the non-HQZMP treated control group, quantity of interstitial mastocyte, focal lymphocytic aggregation and interstitial microvessel damage in the HQZMP treated group were more obvious (P < 0.01). CONCLUSION: HQZMP could improve the immune function of esophageal patients in peri-operational period.

Adjuvants, Immunologic↗
PubMed1997

[Clinical and experimental study on treatment of fatty liver of phlegm-dampness stagnation type by zhiganle capsule].

OBJECTIVE: To explore the mechanism of Zhiganle capsule (ZGLC) in treating fatty liver of Phlegm-Dampness Stagnation type. METHODS: One hundred and forty-six patients were treated with ZGLC, the effects were observed and compared with those treated with Dongbao Gantai (DBGT,) tablets as control. The effect of ZGLC on ethionine induced hyperlipemia model rats was also observed. RESULTS: The effect of ZGLC was obviously better than those of DBGT in relieving or removing symptoms, normalizing the ultrasonic abnormal findings, lowering the serum levels of lipid and enzyme and improving hemorrheologic properties. Experimental study showed that ZGLC could reduce the blood lipids, inhibit the lipid deposition in liver. As compared with the model group, level of triglyceride in the ZGLC treatment group were much lowered (P < 0.01) and the effect was better than those in the control group. CONCLUSIONS: ZGLC has the effect of reducing blood triglyceride level, inhibiting lipid deposition in liver and improving hemorrheologic properties.

Adult↗
PubMed1994

[Treatment of advanced and recurrent gynecologic cancer with chemotherapy by cannula perfusion into both internal iliac arteries].

This paper reports fourteen cases of advanced and recurrent gynecologic cancers treated with prolonged arterial perfusion chemotherapy by catheterization of both internal iliac arteries through femoral arteriopuncture under X-ray. The immediate effective rate was 78.5%, follow-up rate 100%. By this technique anticancer drugs can be selectively injected into vessels supplying the tumors. The drug is concentrated within the tumor resulting in longterm better local effects, and fewer general side reactions, and a much higher dose than that which could be given conventionally. This technique is considered as a new route of treatment in gynecologic tumors.

Adult↗
PubMed1994

Hormonal regulation of oocyte development and maturation of amphioxus.

Using the electron microscopic technique and radioimmunoassay, the hormonal regulation of the oocyte development and maturation of the amphioxus has been investigated. The results indicate that the exogenous GnRH-A can stimulate the oocyte development and maturation as well as the spawning of the amphioxus. It has further been revealed by radioimmunoassay that GnRH-A can increase the context of sex steroid hormone, especially, estradiol-17 beta, by 1-3 times. The increase of estradiol-17 beta coordinates with the synthesis of oocyte into yolk substance. The results further justify that the hormonal regulation in the oocyte development and maturation of amphioxus is similar to that of vertebrates. This is of great theoretical significance for reproductive endocrine physiology of amphioxus.

Animals↗
PubMed1993

[A study on the monoclonal anti-idiotypic antibody NP30 for the diagnosis of schistosomiasis japonica].

In this study the ability of the monoclonal anti-idiotypic antibody NP30 was tested as a substitute of diagnostic antigen in detecting antibody of Schistosoma japonicum from human sera by use of ELISA. The results showed that the seropositive rate was 98% with NP30 in the group of acute infection, which was comparable to 94% with gut associated antigens (GAA) and 98% with the soluble egg antigens (SEA); 87% with NP30 in the group of chronic infection which was comparable to 86% with GAA but lower than that of 98% with SEA. The false positive rate was about 3% for all three diagnostic antigens. The results also showed that the geometric mean titer (GMT) of antibody to NP30 was higher than that to GAA but lower than that to SEA in the acute infection group and the GMT of antibody to NP30 was lower than both those to GAA and to SEA in the chronic infection group, suggesting that the antibody to NP30 appeared earlier and decayed more quickly during the process of infection. The authors suggested that NP30 could be used for the diagnosis of schistosomiasis japonica.

Animals↗
PubMed1993

[Analysis of the protein/polypeptide in Culex pipiens pallens (Diptera:Culicidae) during hibernation by two-dimensional gel electrophoresis].

Analysis on the protein/polypeptide in Culex pipiens pallens (Diptera: Culicidae) caught from the resting or overwintering places during the 3rd ten-day of September, the 2nd ten-day of December and the 1st ten-day of March in next year in Zhengzhou (34 degrees 43' N, 113 degrees 39' E) during hibernation by two-dimensional gel electrophoresis indicated that there were 153, 118 and 169 protein/polypeptide spots in unoverwintering, overwintering and recovering mosquitoes respectively. The molecular weight (MW) of most of the spots was less than 43.0 kDa, with isoelectric points (pI) between 5.65-7.34. The comparisons between each two types of mosquitoes during hibernation revealed that the variant rate of protein/polypeptide spots between overwintering and recovering mosquitoes (31.01%) was significantly higher than that between unoverwintering and recovering females (21.12%) (P < 0.05). The variant spots were 13 in unoverwintering, 7 in overwintering and 27 in recovering mosquitoes respectively. The comparisons among three types of mosquitoes during hibernation showed that the variant rate was significantly higher in recovering females (15.98%) than in unoverwintering (8.50%) (P < 0.05) and overwintering females (5.93%) (P < 0.01). The total variant rate of protein/polypeptide spots of all these three types of mosquitoes was 10.68%. Because the blood meal of all the mosquitoes used for homogenization was at Sella 1 (empty), and the ovary development was under Christophers II (resting stage), the experimental results were not affected by blood meal and the later ovary development (Christophers II-V).

Animals↗
PubMed1993

Immunodiagnosis of Schistosoma japonicum infection in China.

Diagnosis is an essential prerequisite for the control programs of schistosomiasis. Chinese scientists have been making efforts for decades towards the development of immunological approach in an attempt to offer alternatives of or supplements to parasitological diagnosis. The authors outline briefly the main advances in the research of immunodiagnosis for schistosomiasis japonica in China in two catalogues: antibody detection and antigen detection. A number of immunodiagnostic assays based on antibody detection, which have been used widely in the control programs in China, are appraised in terms of sensitivity and specificity, and the achievements in the development of immunodiagnostic assays based on antigen detection are introduced. Readers will find some information concerning the efforts so far made by Chinese scientists in the improvement of immunodiagnosis by use of molecular approach, including search for biochemically and/or immunochemically isolated and/or purified antigens, recombinant antigens and anti-idiotypic reagents of diagnostic interest to construct defined antigen-antibody systems for diagnosis, and development of monoclonal antibody-based assays for antibody or antigen detections.

Animals↗
PubMed1992

[Production and characterization of a murine protective monoclonal antibody against Schistosoma japonicum schistosomula].

Eight murine monoclonal antibodies against surface determinants of Schistosoma japonicum (Chinese mainland strain) schistosomula were generated, of which only one monoclonal IgM antibody (N15D9) gave protection at level ranging from 14 to 39% in experiments of passive transfer or inhibition of infectivity while the others did not exhibit significant levels of passive protection. Further characterization of N15D9 antigen specificity showed that 96 and 14 kDa antigen molecules in cercaria, and 132 and 10 kDa in schistosomula could be recognized by N15D9 in Western blot assay. Furthermore, the 96 and 132 kDa molecules could also be recognized by pooled infected human sera while 14 kDa and 10 kDa only by sera from mice vaccinated with 3-hour schistosomula. The molecules recognizable by N15D9 were surface epitopes repeatedly expressed on cercaria, in vitro 3-hour mechanically transformed schistosomula and 5 day lung-stage schistosomula, as demonstrated by indirect immunofluorescence surface binding assay.

Animals↗
PubMed1991

[The establishment and characterization of the anti-idiotypic monoclonal antibody-NP30 of Schistosoma japonicum].

A hybridoma cell line secreting an IgM monoclonal antibody designated NP30 was obtained from a fusion of SP2/o and spleen cells of a BALB/c mouse chronically infected with schistosoma japonicum for one and a half year and identified by screening with immunized rabbit sera against gut-associated antigen (GAA) and soluble egg antigen (SEA) of S. japonicum, indicating that the NP30 was an anti-anti-antigen or anti-antibody. NP30 was further determined to be an anti-idiotypic antibody (anti-id) which was serologically and functionally identical to GAA, so that it could be portrayed as the internal image of GAA, which might have the potential to be used as an antigenic reagent in immunodiagnostic assays of schistosomiasis japonica.

Animals↗
PubMed1990

Follicular development, oocyte maturity and fertilization in vitro.

Forty-four infertile women in 44 cycles were stimulated by CC/hMG/hCG (39 cycles) and by CC/hCG (5 cycles) for the purpose of in vitro fertilization (IVF, 37 cases with 35 transfers) and gamete intrafallopian transfer (GIFT, 7 cases). Intraoperative ovum pick-up was performed 32-36 hours after hCG injection. Blood E2 and P levels were determined. Blood E2 was found to be proportional to the number of mature and intermediate oocytes obtained, and correlated with the number of follicles (greater than 10 mm) and the total follicular fluid volume. Altogether 213 follicles aspirated were analyzed. There was a highly significant linear correlation (r = 0.72, P less than 0.01) between the follicular diameter (X cm) on the operation day and the aspirated follicular fluid volume (FFV, Y). The equation of the line was Y = 3.8 X-4.4. The higher concentrations of follicular E2, P, FSH, LH and lower T concentration were crucial to the maturing process of oocytes. It was found that mature oocytes could be retrieved from 26.5% of the small follicles, so it is worthwhile to aspirate the small ones. The fertilization rate was much higher with the mature oocytes than the immature ones. But the morphologically mature oocytes might function differently, i.e. the mature oocytes retrieved from the small follicles or from follicles with unbalanced hormonal microenvironment usually give a low fertilization rate.

Adult↗
PubMed1990

Laparotomy ovum pick-up and ultrasonic transvaginal ovum retrieval in an IVF program.

Different methods of ovum pick-up were used in an IVF program from January 1, 1988 to January 31, 1989. In the initial stage of the program, laparotomy ovum pick-up with surgical treatment of pelvic diseases resulted in two cases of clinical pregnancy in 1987 with full-term delivery of two healthy babies. Transvaginal ovum pick-up with a needle using an ultrasonic endovaginal transducer was started in late 1987. In 1988-1989 the transvaginal method was employed more frequently than laparotomy, and it has become a routine practice in the IVF program. The ultrasonic endovaginal approach seemed to give a low ovum recovery rate (70.99%), but the fertilization rate was high (85.10%, P less than 0.05), and the rate of embryo transfer was 89.33%. The average number of pre-embryo per transfer was 2.26. These figures were quite similar in the two groups. The ultrasonic transvaginal approach for egg retrieval was noninvasive, easily accepted by patients. We suggest that it can be done repeatedly on one patient to improve the cumulative pregnancy rate. However, the laparotomy for ovum pick-up has shown some special merits and is still indicated in some cases.

Adult↗
PubMed1990

[Preovulatory human oocyte and its hormonal microenvironment in stimulated cycles].

The hormonal microenvironment of 213 follicles in 44 stimulated cycles was analysed. Mature and intermediate oocytes were found to be associated with larger follicles which contained significantly higher levels of E2, P, FSH and LH, while smaller follicles containing significantly higher T level usually gave rise to immature oocytes. The fertilization rate was higher in mature and intermediate oocytes than in immature oocytes. In this study there was no significant difference in the cleavage rate of the fertilized eggs in relation to the E2, P, T, FSH and LH concentrations in follicular fluid as well as to the contents of these hormones per follicle.

Adult↗
PubMed1990

Studies on specific serological antigens in metacercariae and juveniles of Paragoniums westermani and its monoclonal antibodies.

The polyclonal antibodies to juveniles of Paragoniums westermani (PwJ-PcAbs) from sera of Wistar rats infected with Paragoniums westermani (P.w.) were purified by Sephadex G 200 chromatography. Next the shared serological antigens of P.w. metacercaria and juveniles (PwMJ-SAg) from the crude antigens of the metacercariae (M-NS-Ag) were purified with immuno-affinity chromatography on cyanogen bromide-activated cross-linked Sepharose 4B beads coupled with PwJ-PcAbs. PwMJ-SAg, a group of glycoprotein molecules shown by the staining test, were specific serological antigens of P.w. metacercariae and juveniles, identified by the immunoabsorb test and immunoelectrophoresis. By SDS-PAGE, PwMJ-SAg were fractionated to seven bands, including major bands A (27.5 K) and Bi (19.5 K), the two major serological antigen molecules. 20 sera samples from the patients with the nonpulmonary type of P. w. paragonimiasis were detected using PwMJ-SAg and M-NS-Ag by Dot-ELISA, and the difference of sensitivity between two antigens was highly statistically significant (P less than 0.001). BALB/c mice, in the early stage of infection with P. w. metacercaria, were immunized with PwMJ-SAg. The spleen cells of the mice were isolated and fused with SP2/o, a murine myeloma cell line. After three subclonal cultures, eight cell lines secreting monoclonal antibodies (McAbs) to PwMJ-SAg were prepared from 384 wells of hybridoma cells. All McAbs were IgG1 subclass.

Animals↗
PubMed1989

[Cellular responses elicited by challenged schistosomula of Schistosoma japonicum in the skin of naive and chronically infected mice].

Naive and chronically infected C57BL/6 mice were challenged percutaneously over the ear pinna with Schistosoma japonicum cercariae. After 15 hours, the number of EOS increased significantly in the skin of chronically infected mice. Inflammatory cells aggregated in the vicinity of schistosomula or entrapped intact and disintegrated schistosomula, forming granulocytic micro-abscesses in both groups. Ultrastructure studies revealed that flattened EOS tightly attached to the schistosomulum surface and degranulated. Local tegument damage occurred in the area of attachment. NEU adherence did not seem to be as intimate as EOS, and degranulation was not seen. The tegument of the attached schistosomulum remained normal. The result suggested that EOS appeared to be the efficient killer cell against skin phase schistosomula of S. japonicum.

Animals↗