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Biomedical subjects

W Zalewski

Publications and source records attributed to W Zalewski.

18 recordsLinked to original sources

The effect of Saccharomyces cerevisiae1026 used alone or with vitamin-mineral premix on biochemical parameters of blood and milk in dairy cows.

When evaluating the effects of yeast culture (Saccharomyces cerevisiae1026) supplied with or without a vitamin premix and mineral bioplexes on some intermediates and end-products involved in the synthesis of milk constituents in 30 early-lactation Black and White Lowland cows, no significant differences were found in the glucose level, mineral contents and enzyme activities of the blood serum. The effect of yeast culture on the availability of minerals for milk synthesis depended upon the dynamics of degradation of mineral bioplexes in the rumen and the cows' mineral status. The insignificant increase found in blood total protein content and the simultaneous small differences in blood urea nitrogen (BUN) and milk urea nitrogen (MUN) values in cows supplied with the yeast culture were probably associated with a high ammonia incorporation into microbial protein in the rumen, which increased protein supply for milk protein synthesis and decreased the nitrogen loss.

Alanine Transaminase↗

The effect of Saccharomyces cerevisiae1026 used alone or with vitamin-mineral premix on milk yield and milk composition in dairy cows.

The effect of a yeast culture (Saccharomyces cerevisiae1026) added to the diet with or without supplementation with vitamin premix and mineral bioplexes on milk production responses of 30 early-lactation Black and White Lowland cows was determined in a 17-week experiment. The daily ration (DM) consisted of 55% forages and 45% concentrates. Milk yield and milk composition were only slightly influenced by the yeast culture given without premix, but combined supplementation with yeast culture and premix exerted a significant effect on milk production and composition. Cows supplied with the yeast culture produced 38 kg more milk than the controls by day 120 of lactation, whereas from cows fed yeast culture and premix an additional 150 kg of milk was obtained. In cows treated with the yeast culture alone, the total yield of milk protein, casein and lactose rose by 4.4 kg, 3.0 kg and 6.0 kg, respectively, and that increase was not significant. At the same time, the total yield of milk components increased significantly in cows fed a combination of yeast culture, vitamin premix and mineral bioplexes. The total yield of milk protein, casein and milk fat increased by 9.2 kg, 6.0 kg and 8.2 kg, respectively. The results suggest that mineral bioplexes stimulate the action of yeast culture in the rumen and the availability of nutrients in the mammary gland.

Animal Feed↗

[Osteopoikilosis--case report].

A case of osteopoikilosis in 15 years old female with painful hip is presented. Genetic background of this condition has been confirmed by finding mother, two brothers and sister of the patient who affected with osteopoikilosis.

Adolescent↗

[Value of harmful elements in fruit and vegetables grown in the province of Siedlce].

The content of iron, manganese, nickel, lead, copper, zinc and cadmium was determined by means of atomic absorption spectrometry (ASA) in vegetables and fruit grown in the Province of Siedlce. The studied fruits included tomatoes, cucumbers, cabbages, carrots, parsley, celery, red beets, potatoes garden strawberries, black and red currants, raspberries, apples, plums and cherries. The total number of studied samples of fruits and vegetables was 229. In 49 studied samples cadmium exceeded 0.03 mg/kg and in 4 samples lead was above 0.3 mg/kg. In most studied samples of green parts of parsley and celery (80%) zinc was above 10 mg/kg of fresh mass, that is the level permitted in our country for products containing below 20% of dry mass.

Environmental Exposure↗

[Determination of elements hazardous to health in vegetables grown in the Siedlece district].

In the years 1986-1987 the content of iron, manganese, zinc, copper, nickel, lead and cadmium was determined by the atomic absorption spectrometry method in vegetables grown by gardeners in Wegrów, Sokołów Podlaski and Siedlce. These elements were measured in cabbages, tomatoes, carrots, parsley, celery (root and leaves), red beet and potatoes. The content of lead in these vegetables was from 0.0 to 0.596 mg/kg, and that of cadmium was from 0.002 to 0.184 mg/kg (fresh mass). The level of cadmium in many samples of vegetables exceeded to limit permitted by the Polish Ministry of Health. Lead level in a sample of parsley leaves was two times above the permitted value.

Agriculture↗

[Effect of the methods of preparing aqueous extracts on the amount of soluble compounds in fascial prostheses].

Methods of testing products made of plastics in medicine do not take into account chemico-toxicological evaluation of biomaterials made of fibres or of foam plastics. According to the project of the monograph Compendium Medicamentorum COMECON, water extracts should be used, where the is proportion would be maintained: 30 g of biomaterial in 100 cm3 of water. The proportion, in case of products with well-developed surface, does not ensure that the material is wholly immersed in fluid. Therefore studies were made on the use of lower concentration of the material in water, with on the other hand, extraction time being prolonged. Summing up, the authors suggest, with regard to grafts, use of the following conditions of water extraction: temperature 120 degrees C, duration 60 min. proportion of the studied material in the amount of distilled water 1:10.

Biocompatible Materials↗

Influence of vagal input on cerebral blood flow in monkeys and dogs after experimental cerebral vasospasm.

Cerebral blood flow (CBF) was a response to experimental subarachnoid haemorrhage (SAH) in four monkeys ,and three dogs. Decrease in CBF was observed in the white and gray matter and in the flow measured within the internal carotid artery as the result of SAH. Bilateral vagotomy enhanced CBF in the gray matter and blood flow in the internal carotid artery to values exceeding the control ones. Results suggest the existence of a receptor susceptible to subarachnoid haemorrhage and connected with vagal nuclei.

Animals↗

[Physico-chemical and biological studies of plastic materials intended for long-term contact with blood].

The criteria used presently for evaluating the materials for haemotherapy, which are based on chemical, physicochemical and biological extracts of aqueous solutions have been found to differ from the results observed after actual prolonged contact of these materials with blood and its elements. All the investigated plastic materials complied with the required quality standards, and, despite this, some of them, such as natural latex or siliconized rubber, caused considerable adhesion of proteins, release of high amounts of haemoglobin from the erythrocytes, and have been found even most thrombogenic of all 8 plastic materials tested. Investigations by means of scanning microscopy demonstrated that adhesion of fibrinogen and thrombocytes to the surface of the plastic materials depended to a high extent on the smoothness of this surface. It depended also on migration of components specific for each material. This was also the cause of variations in the adsorption of albumin, globulin, and fibrinogen labelled with 125I after their mixing with plasma.

Adhesiveness↗

Comparison of three methods of toxicity evaluation of medical plastics.

A comparative study on the toxicity of various kinds of plastic and rubber materials (PVC and PE tubes, rubber stoppers) used in medicine and pharmacy was performed. As biological material for testing served: cultures of normal human fibroblasts, aquarium fish (Lebistes reticulatus/sp.) and bull and rat spermatozoa. Almost full correlation between the results of testing on fish cuture cells and bull spermatozoa has been found. Heterogeneity of rat spermatozoa (various stages of maturity) make useless of this biological material. The most simple method of toxicity evaluation is provided by the use of aquarium fish. In fibroblast cultures serum extracts show a higher degree of cytotoxicity than water extracts.

Animals↗