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WL Straube

Publications and source records attributed to WL Straube.

2 recordsLinked to original sources
PubMed2000

Use of a chiA probe for detection of chitinase genes in bacteria from the Chesapeake Bay(1).

PCR primers specific for the chiA gene were designed by alignment and selection of highly conserved regions of chiA sequences from Serratia marcescens, Alteromonas sp., Bacillus circulans and Aeromonas caviae. These primers were used to amplify a 225 bp fragment of the chiA gene from Vibrio harveyi to produce a chiA gene probe. The chiA PCR primers and probe were used to detect the presence of the chiA gene in an assemblage of 53 reference strains and gave consistent results. Selected chiA fragments amplified by PCR were cloned and sequenced from nine known strains and from Chesapeake Bay isolates 6d and 11d. This confirmed the specificity and utility of the primers for detection of chiA-positive environmental strains. Over 1000 bacterial isolates from Chesapeake Bay water samples were tested for the presence of the chiA gene which was found to be present in 5-41% (average 21%) of the culturable bacterial community. The approach developed in this study was valuable for isolation and enumeration of chiA-positive bacteria in environmental samples.

Journal Article↗
PubMed2000

Ultrasound field estimation method using a secondary source-array numerically constructed from a limited number of pressure measurements

A new and faster method for the accurate estimation of acoustic fields of underwater ultrasonic transducers was developed, tested experimentally, and compared to previously reported methods. Using a limited number of pressure measurements close to the transducer's face, the method numerically constructs a virtual secondary source-array whose acoustic field is similar to the field generated by the actual transducer (primary source). The measured data are used to obtain the normal particle velocity on the surface of the virtual secondary source-array, which in turn permits the calculation of the forward propagating field using the Rayleigh-Sommerfeld diffraction integral. The method is novel in that it constructs a virtual secondary source-array, thus eliminating the problems associated with obtaining the excitation source of a real transducer; and it is faster because it uses finite differences instead of a matrix inversion to obtain the excitation source. Results showed that predicted ultrasound fields agreed quantitatively and qualitatively with measured fields for three commonly used transducer types: two planar radiators (one circular, 0.5 MHz, 1.9-cm diam.; and one square, 1 MHz, 1.2 cm on a side), and a sharply focused radiator (1.5 MHz, 10-cm diam., 10-cm radius of curvature). The agreements suggest that the secondary source-array method (SSAM) is applicable to a wide range of radiator sizes, shapes, and operating frequencies. The SSAM was also compared to these authors' previous equivalent phased array methods (EPAM) [J. Acoust. Soc. Am. 102, 2734-2741 (1997); and Concentric ring equivalent phased array method (CREPAM), UFFC 46, 830-841 (1999)] which require matrix inversions. The SSAM proved to be much faster and equally or more nearly accurate than the previous methods.

Journal Article↗