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Biomedical subjects

Wanda Stokowska

Publications and source records attributed to Wanda Stokowska.

11 recordsLinked to original sources
PubMed2006

The periodontal status of pre-dialysis chronic kidney disease and maintenance dialysis patients.

BACKGROUND: Periodontitis contributes to generalized inflammation and development of systemic diseases, including atherosclerosis and cardiovascular disease. Its extent in maintenance haemodialysis (HD) patients is disputable and not known in continuous ambulatory peritoneal dialysis (CAPD) and pre-dialysis chronic kidney disease (CKD) patients. METHODS: One hundred and six patients (35 on HD, mean age, 56 years; 33 on CAPD, mean age 51 years; and 38 pre-dialysis CKD stage 2-5, mean age 51 years) from north-eastern Poland were enrolled. Dialysis subjects were recruited from a cohort of 141 HD and 61 CAPD patients. Two control groups comprised 26 generally healthy individuals with advanced periodontitis requiring specialized treatment, and 30 subjects from general population. Gingival index (GI), papillary bleeding index (PBI), plaque index (PI), loss of clinical attachment level (CAL) and community periodontal index of treatment needs (CPITN) were determined according to WHO recommendations. RESULTS: Average values of the indices in HD, CAPD, pre-dialysis CKD, advanced periodontitis and general population subjects were as follows: GI-1.37, 0.95, 1, 2 and 1; PBI-1.45, 0, 0, 2.20 and 1; PI-2.05, 1.59, 1, 2 and 1; and CAL loss-5.11, 3.47, 2.50, 4.68 and 1.40 mm, respectively. CPITN, analysed separately as community periodontal index and periodontal treatment needs, further indicated a high severity of periodontitis in all renal failure groups as compared with general population subjects. The disease was most advanced in maintenance HD patients-comparable to the full-symptomatic form of periodontitis; then it was successively diminished in CAPD and pre-dialysis CKD subjects. CONCLUSIONS: Periodontal disease is prevalent, severe and under recognized in renal failure patients. Prophylaxis and early dental treatment should be intensified in these subjects, and may be of interdisciplinary importance.

Adult↗
PubMed2006

Hepatocyte growth factor in saliva is a potential marker of symptomatic periodontal disease.

Evidence has been emerging that hepatocyte growth factor (HGF) - a pluripotential regenerative cytokine - is a key factor in the pathogenesis and progression of periodontal disease, mostly through its over-stimulation of gingival epithelial cell growth and impairment of the regeneration of collagenous structures. We measured the levels of immunoreactive HGF in unstimulated whole mixed saliva from 26 patients referred for treatment of periodontal disease, and from 20 healthy subjects. HGF was detected in all saliva samples from the patients, the concentration ranging from 0.06 to 5.38 ng/ml, with a mean concentration of 1.87 +/- 1.32 ng/ml. In healthy individuals, the median salivary HGF level was 0.68 ng/ml (range: 0 - 7.33 ng/ml), being almost 3-fold lower (P < 0.0001) than that in the patients. Periodontal parameters in the patients were: gingival index (GI) 2.0 (0 - 2.8), papillary bleeding index (PBI) 2.2 (0 - 3.2), plaque index (PI) 2.0 (0 - 3.0), probing depth (PD) 3.0 (1.8 - 5.9) mm, and loss of clinical attachment level (CAL) 4.7 (1.1 - 10.6) mm. We found that the salivary HGF level was positively correlated with GI (P = 0.004), PBI (P = 0.046) and PI (P = 0.001), but not with PD (P = 0.351), CAL loss (P = 0.172), number of teeth (P = 0.279) or patient age (P = 0.362). Our findings suggest that salivary HGF concentration may be a novel marker of symptomatic periodontal disease, and that it warrants further validation.

Biomarkers↗
PubMed2006

[Dietary habits and early childhood caries intensity among young children].

The aim of this study was to evaluate the influence of a diet and the feeding method on caries intensity among children aged 36-48 months. Dental examination was carried out in 255 children and their mothers were asked about child's dietary habits. The population checked was divided into three groups: with dmf = 0, dmf 1-3 and dmf > or = 4. Statistically significant correlation between caries intensity and bottle feeding during sleep and frequency of eating cariogenic food were shown. Parents of young children should be educated about the influence of dietary habits on dental condition.

Catchment Area, Health↗
PubMed2005

[Metabolism of tryptophan via the kynurenine pathway in saliva].

In this review, the role of the kynurenine pathway enzymes TDO (tryptophan 2,3 dioxygenase) and IDO (indoleamine 2,3 -dioxygenase) as well as the properties of the metabolites of kynurenine degradation present in human saliva described. The implications of these metabolites in the pathomechanism of tissue and organs dysfunction are demonstrated. The authors also describe at length the role of saliva kynurenine derivatives in the onset and development of periodontal disease in humans.

Humans↗
PubMed2004

Fluoride concentration in superficial enamel layers of permanent teeth in children residing in areas with different levels of environmental pollution with fluorine compounds.

The concentration of fluoride in superficial layers of enamel of permanent teeth was studied in 14-year-old children residing in Szczecin and Bialystok-two cities differing regarding the level of environmental fluorine pollution. It was found that higher environmental pollution with fluorine favors the accumulation of this element in superficial layers of enamel in the form of structural fluoride. Total and structural fluoride concentration decreased with layer depth.

Child↗
PubMed2003

[Periodontal disease and atherosclerosis: an underestimated link?].

Periodontal disease is a common bacterial and destructive disorder of oral tissues. We reviewed epidemiological and experimental to data studies demonstrating close associations between chronic periodontitis and development of generalized inflammation, vascular endothelial injury, and atherosclerosis. Periodontal disease has been convincingly emerging as an important independent cardiovascular risk factor. It deserves timely treatment also as a likely part of primary prevention of ischemic heart disease, stroke, and peripheral vascular disease.

C-Reactive Protein↗
PubMed2002

The influence of cervical lesion on the stress state and strength of tooth with occlusal restoration: a numerical model study.

This paper is concerned with bio-engineering problems in dentistry. The analysis of stress in a lower premolar with a deep non-carious cervical lesion and restored occlusal Class I cavity under occlusal load of 100 N was shown. Finite element method was used for the analysis of a two-dimensional model of a human tooth. In order to facilitate the evaluation of the loads, four cross-sections in characteristical points of the model were made. The model was generated and the calculations were done on IBM Pentium III personal computer. The principal and equivalent stresses were calculated and used in qualitative and quantative evaluation of tooth tissue and tooth restoration strength. The results are shown in figures and charts. The analysis of the cross-sections distant to the cervical area has proved that the presence or absence of cervical lesion has no significant influence on the values and stress distribution. Only in the region of sharp-shaped notch in the bottom of defect (4-4) high stresses in the tooth tissues occur. The values of stress did not exceed the compressive and tensile strength in any case under investigation. The study has shown that the presence of cervical angular lesion does not affect the mechanical strength of a tooth with a properly restored occlusal cavity. Clinically, the situation is safe from the biomechanical point of view.

Biomechanical Phenomena↗
PubMed2002

Evaluation of lymphocyte populations and subpopulations extracted from inflamed periodontal tissues.

The aim of the present study was evaluation the distribution of B- and T-cells and T-cell subsets in periodontal tissues from patients with different periodontitis forms. Periodontal tissue samples were collected from group P patients during routine surgical procedures, while from group C during tooth extraction for orthodontic or prosthetic purposes. Directly after collection, tissue samples were placed in a criostat or fixed in 10% buffered formalin for 24 h at room temperature. Following fixation the material was embedded in paraffin and subjected to routine histological techniques. Examinations of B- and T-lymphocytes populations and T-lymphocytes subsets were made with the use of immunohistochemical method. In C group single T and B lymphocytes were found in histological examination in pocket epithelium zones. In early onset periodontitis (EOP) patients in inflammatory infiltration lymphocytes T were dominating while in adult periodontitis (AP) patients dominating were B lymphocytes. Mean CD4/CD8 ratio in control group was 1.7 and in EOP and AP patients 1.1 and 2.6 respectively.

Adult↗
PubMed2002

[Paracervical non-caries defects].

The aim of the study was the description of origin and treatment of paracervical, non-caries defects. Dental plaque is involved in the formation of caries defects, while those of non-caries origin are produced due to the action of mechanical and chemical factors. The treatment consists in wide fluoride prophylaxis (elimination of causes) and in the application of mineralization procedures and restoration of missing dental tissue using modern dental highly-adhesive materials. At the same time occlusion disorders and parafunctions are eliminated.

Dental Prophylaxis↗
PubMedDate not supplied

Evaluation of selected peripheral blood leukocyte functions in patients with various forms of periodontal disease.

INTRODUCTION: Periodontitis (P) is an infectious disease that develops in the supporting tissues of the tooth. One of the risk factors leading to it may be dysfunction of some immune system cells. Therefore, the object of the study was to assess selected functions of peripheral blood leukocytes in patients with various forms of P. As leukocytes are able to secrete interleukin (IL)-4 and IL-6, concentrations of their soluble receptors and the expression of their membrane receptors were investigated. MATERIAL AND METHODS: Twenty generally healthy subjects with aggressive (AP)and chronic periodontitis (CP)were enrolled in the study. The control group consisted of 8 healthy subjects,with no changes in periodontium. Peripheral blood mononuclear cells (PBMCs) were isolated and cultured. Levels of IL-4,IL-6,and their soluble receptors sIL-4R and sIL-6R were determined in the supernatant by ELISA. The expressions of cell surface IL-4R and IL-6R were assayed on PBMC using flow cytometry. RESULTS: No statistically significant differences were found in the selected parameters between people with periodontal disease and healthy controls. However, in subjects with AP, there was an increasing tendency in IL-6 concentration and IL-4R expression on PBMCs. CONCLUSIONS: Our results show that leukocytes play a significant part in P and their activity is probably lesion-dependent. Estimation of the cytokines secreted by leukocytes may facilitate differentiation and prognosis of the disease progression.

Adult↗